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FIGURE: 1 / 3
AHCY Antibody (MA5-42797) in WB
Western blot analysis of AHCY in extracts of various cell lines. Samples were incubated with AHCY Monoclonal antibody (Product # MA5-42797) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
MA5-42797
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4E9H10
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human AHCY (P23526).
Conjugate
Form
Liquid
Concentration
0.33 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911938
Product Specific Information
Positive test controls include: HT-29, HepG2, A375, K-562, Mouse pancreas, Mouse liver, Mouse kidney, Rat kidney. The target is usually found in the following locations: Cytoplasm, Melanosome.
Immunogen sequence: GHFDVEIDVK WLNENAVEKV NIKPQVDRYR LKNGRRIILL AEGRLVNLGC AMGHPSFVMS NSFTNQVMAQ IELWTHPDKY PVGVHFLPKK LDEAVAEAHL G
Target Information
S-adenosylhomocysteine hydrolase (AHCY) catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acetylation; Adenosylhomocysteinase; AdoHcyase; CUBP; Hydrolase; Liver copper-binding protein; NAD; Polymorphism; S-adenosyl-L-homocysteine hydrolase; S-adenosylhomocysteine hydrolase
Gene Aliases: AA987153; adoHcyase; AHCY; AL024110; CuBP; SAHH
UniProt ID: (Human) P23526, (Mouse) P50247, (Rat) P10760
Entrez Gene ID: (Human) 191, (Mouse) 269378, (Rat) 29443
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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