Invitrogen

AHR Monoclonal Antibody (4MEJJ), PE, eBioscience™

FIGURE: 1 / 4

AHR Antibody (12-5925-82) in Flow

Left: Splenocytes from C57BL/6 mice (WT, red histogram) or AHR-knockout mice (KO, black histogram) were polarized under Th17 conditions for 3 days. Cell were stained with Fixable Viability Dye eFluor® 780 (Product # 65-0865-14), fixed and permeabilized with IC Fixation & Permeabilization Buffers (Product # 00-8222 and Product # 00-8333-56), and then intracellularly stained with Anti-Mouse CD4 PerCP-eFluor® 710 (Product # 46-0042-82) and 1.0 µg of Anti-Mouse AHR PE. Viable CD4+ cells were used for analysis. Right: C57BL/6 splenocytes were p... View More

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Product Details

12-5925-82

Applications

Tested Dilution

Publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 5 publications 5 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

4MEJJ

Immunogen

E. coli derived, His-tagged AHR (aa 443-805)

View immunogen

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572644

Product Specific Information

Description: This 4MEJJ monoclonal antibody recognizes mouse aryl hydrocarbon receptor (AHR). The AHR is a ligand-activated transcription factor that mediates the toxic effects of a diverse group of environmental contaminants, most notably aryl hydrocarbons such as polychlorinated biphenyls (PCB) and tetrachlorodibenzo-p-dioxin (TCDD). The AHR has also been shown to bind to a number of naturally occurring compounds found in fruits and vegetables as well as compounds generated through normal cellular metabolism. AHR is localized in the cytoplasm in a complex that includes HSP90, p23, and XAP2/AIP/ARA9. Upon ligand-binding, AHR translocates to the nucleus and binds with aryl hydrocarbon receptor nuclear translocator (ARNT), and this complex binds to the consensus DNA sequence, GCGTG, found in the promoter/enhancer regions of many genes such as CYP1A1. The AHR is expressed in many cell types, with highest expression levels found in liver. The AHR has been shown to play a role in the regulation/differentiation of Treg and Th17 cells.

The 4MEJJ monoclonal antibody has been found to recognize the b-1, b-2 and d alleles of mouse AHR but does not crossreact with human AHR.

Applications Reported: This 4MEJJ antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 4MEJJ antibody has been tested by intracellular staining and flow cytometric analysis of 3-day Th17-polarized mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

AHR (Ah Receptor) belongs to a family of proteins comprised of its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM. AHR contains an N-terminal sequence of approximately 200 amino acids termed the PAS domain. AHR, found in a variety of tissues, binds to a specific DNA enhancer sequence and initiates transcription of the mRNA for the cytochrome P-450 (CYPIA1) gene. The gene for AHR encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450, and its ligands included a variety of aromatic hydrocarbons. AHR is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for AHR. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form, and this transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The aryl hydrocarbon receptor directs the differentiation of murine progenitor blastomeres.

Cell biology and toxicology

Ko CI,Biesiada J,Zablon HA,Zhang X,Medvedovic M,Puga A

12-5925-82 was used in Immunohistochemistry-immunofluorescence to appear that AHR directs the differentiation of progenitor blastomeres and that disruption of preimplantation AHR functions may significantly perturb embryogenesis leading to long-lasting conditions at the heart of disease in offspring's adulthood.

Tue Aug 01 00:00:00 EDT 2023

Microbiota-assisted iron uptake promotes immune tolerance in the intestine.

Nature communications

Zhu L,Li G,Liang Z,Qi T,Deng K,Yu J,Peng Y,Zheng J,Song Y,Chang X

12-5925-82 was used in Flow cytometry/Cell sorting to reveal an association between nutrient uptake and immune tolerance in the intestine.

Mon May 15 00:00:00 EDT 2023

The neutrophil antimicrobial peptide cathelicidin promotes Th17 differentiation.

Nature communications

Minns D,Smith KJ,Alessandrini V,Hardisty G,Melrose L,Jackson-Jones L,MacDonald AS,Davidson DJ,Gwyer Findlay E

Published figure using AHR monoclonal antibody (Product # 12-5925-82) in Flow Cytometry

Wed Feb 24 00:00:00 EST 2021

Blockade of the AHR restricts a Treg-macrophage suppressive axis induced by L-Kynurenine.

Nature communications

Campesato LF,Budhu S,Tchaicha J,Weng CH,Gigoux M,Cohen IJ,Redmond D,Mangarin L,Pourpe S,Liu C,Zappasodi R,Zamarin D,Cavanaugh J,Castro AC,Manfredi MG,McGovern K,Merghoub T,Wolchok JD

12-5925 was used in Flow cytometry/Cell sorting to show that the AHR pathway is selectively active in IDO/TDO-overexpressing tumors and is associated with resistance to immune checkpoint inhibitors.

Tue Aug 11 00:00:00 EDT 2020

Blockade of the AHR restricts a Treg-macrophage suppressive axis induced by L-Kynurenine.

Nature communications

Campesato LF,Budhu S,Tchaicha J,Weng CH,Gigoux M,Cohen IJ,Redmond D,Mangarin L,Pourpe S,Liu C,Zappasodi R,Zamarin D,Cavanaugh J,Castro AC,Manfredi MG,McGovern K,Merghoub T,Wolchok JD

12-5925 was used in Flow cytometry/Cell sorting to show that the AHR pathway is selectively active in IDO/TDO-overexpressing tumors and is associated with resistance to immune checkpoint inhibitors.

Tue Aug 11 00:00:00 EDT 2020

Checkpoint Receptor TIGIT Expressed on Tim-1⁺ B Cells Regulates Tissue Inflammation.

Cell reports

Xiao S,Bod L,Pochet N,Kota SB,Hu D,Madi A,Kilpatrick J,Shi J,Ho A,Zhang H,Sobel R,Weiner HL,Strom TB,Quintana FJ,Joller N,Kuchroo VK

12-5925 was used in Flow cytometry/Cell sorting to show that mice with B cell-specific Tim-1 deletion develop tissue inflammation in multiple organs including spontaneous paralysis with inflammation in the central nervous system (CNS).

Tue Jul 14 00:00:00 EDT 2020

7,12-Dimethylbenz(a)anthracene-induced myelotoxicity differs in mice selected for high or low acute inflammatory response: relationship with aryl hydrocarbon receptor polymorphism.

International journal of toxicology

Katz IS,Albuquerque LL,Suppa AP,de Siqueira DM,Rossato C,da Silva GB,Jensen JR,Starobinas N,Cabrera WH,De Franco M,Borelli P,Ibañez OM,Ribeiro OG

Tue Nov 18 00:00:00 EST 2014

Bioinformatics

Protein Aliases: Ah receptor; Aryl hydrocarbon receptor; dioxin receptor

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Gene Aliases: Ah; Ahh; Ahr; Ahre; bHLHe76; In

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UniProt ID: (Mouse) P30561

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Entrez Gene ID: (Mouse) 11622

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Function(s)

DNA binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding signal transducer activity aryl hydrocarbon receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding transcription factor binding enhancer binding sequence-specific DNA binding transcription regulatory region DNA binding protein heterodimerization activity protein dimerization activity Hsp90 protein binding E-box binding basic helix-loop-helix transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter cell morphogenesis ovarian follicle development blood vessel development patterning of blood vessels B cell homeostasis liver development B-1 B cell homeostasis blood vessel remodeling lymphocyte homeostasis immune system process negative regulation of systemic arterial blood pressure cardiac left ventricle morphogenesis circumferential growth involved in left ventricle morphogenesis transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter xenobiotic metabolic process response to stress cell cycle signal transduction blood circulation regulation of blood pressure response to xenobiotic stimulus response to toxic substance regulation of gene expression response to organic cyclic compound B cell differentiation intracellular receptor signaling pathway prostate gland development regulation of B cell proliferation circadian regulation of gene expression embryonic hemopoiesis post-embryonic hemopoiesis camera-type eye development T cell homeostasis positive regulation of cell size negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of RNA polymerase II transcriptional preinitiation complex assembly negative regulation of vasoconstriction positive regulation of growth rhythmic process blood vessel morphogenesis spleen development reproductive structure development gland development smooth muscle tissue development regulation of blood vessel size regulation of heart growth negative regulation of necrotic cell death venous blood vessel development kidney morphogenesis common bile duct development cellular response to cAMP glomerulus morphogenesis

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AHR Monoclonal Antibody (4MEJJ), PE, eBioscience™

AHR Antibody (12-5925-82) in Flow

Product Details

12-5925-82

Immunohistochemistry (PFA fixed) (IHC (PFA))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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