Invitrogen

AHR Monoclonal Antibody (RPT9)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

18 Published Figures

38 References

View all (46) AHR antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

AHR Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 26

AHR Antibody (MA1-513) in ICC/IF

Immunofluorescent analysis of Aryl Hydrocarbon Receptor using Aryl Hydrocarbon Receptor Monoclonal Antibody (RPT9) (Product # MA1-513) shows staining in A2058 Cells. Aryl Hydrocarbon Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Aryl Hydrocarbon Receptor (Product # MA1-513) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated... View More

AHR Antibody in Immunohistochemistry (IHC)

AHR Antibody in Western Blot, Immunohistochemistry (WB, IHC)

AHR Antibody in Western Blot, Immunoprecipitation (WB, IP)

AHR Antibody in Immunoprecipitation (IP)

Product Image Gallery

AHR Antibody — Fig. 5 Total amounts of endogenous AhR in the cytosol and nucleus of HeLa cells. (A) HeLa cells were treated with 0.05% DMSO (-) or 1 muM beta-NF (+) for 0, 15, 30, 60, and 120 min at 37 degC. The lysate was collected and centrifuged for 60 min at 105,000 g , then the supernatant was collected. AhR in the cytosol were analyzed by immnoblotting with an anti-AhR antibody or an anti- beta-actin antibody ( n = 4, * p < 0.05). The AhR/beta-actin ratio was quantified using Image J software (Drop of Wisdom). (B) HeLa cells were treated with 0.05% DMSO (-) or 3 muM beta-NF (+) for 30 min at 37 degC. The lysate was collected and centrifuged, then the supernatant was collected. Immunoprecipitation was performed using an anti-Ahr antibody or anti-HSP90 antibody followed by immunoblotting with an anti-HSP90 antibody or an anti-AhR antibody. In panel, the HeLa cell lysate (left two lanes) or immunoprecipitated samples (right two lanes) were separated by SDS-PAGE followed by immnoblotting. The subunit molecular weight of HSP90 and AhR are 90 and 110 kDa, respectively. The 50- and 32-kDa protein bands were the IgG heavy- and light chain, respectively. M, molecular marker proteins. C, HeLa cells were treated with 0.05% DMSO (-) or 1 muM beta-NF (+) for 0, 15, 30, 60, 120, and 360 min at 37 degC. The lysate was collected and centrifuged for 60 min at 105,000 g , then the supernatant was collected as a cytosol fraction. Or the lysate in 2.2-M sucrose solution was centrifuged for 60 min at 30,0 {TM}

Product Details

MA1-513

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-dependent

View 3 publications 3 publications

Flow Cytometry (Flow)

1:50

ELISA (ELISA)

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

View 3 publications 3 publications

ChIP assay (ChIP)

1:100

View 7 publications 7 publications

Gel Shift (GS)

Assay-dependent

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG3, kappa

Class

Monoclonal

Type

Antibody

Clone

RPT9

Immunogen

Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of mouse AhR.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2223958

Product Specific Information

MA1-513 detects the aryl hydrocarbon receptor (AhR) from human, mouse, and rat cells.

MA1-513 has been successfully used in immunoprecipitation, chromatin immunoprecipitation, immunocytochemistry, immunohistochemistry, immunofluorescence, and gel shift procedures. This antibody specifically immunoprecipitates a single ~95 kDa protein representing AhR from Hepa 1 cytosol. Although MA1-513 can be used in Western blotting procedures, MA1-514 (clone RPT1) is recommended for this procedure.

The MA1-513 immunogen is a synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of mouse AhR.

Target Information

AHR (Ah Receptor) belongs to a family of proteins comprised of its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM. AHR contains an N-terminal sequence of approximately 200 amino acids termed the PAS domain. AHR, found in a variety of tissues, binds to a specific DNA enhancer sequence and initiates transcription of the mRNA for the cytochrome P-450 (CYPIA1) gene. The gene for AHR encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450, and its ligands included a variety of aromatic hydrocarbons. AHR is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for AHR. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form, and this transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Microglial aryl hydrocarbon receptor enhances phagocytic function via SYK and promotes remyelination in the cuprizone mouse model of demyelination.

Journal of neuroinflammation

Wang Y,Sun J,Zhu K,Wang D,Zhao X,Zhang H,Wu S,Wang Y,Wang J

MA1-513 was used in Immunohistochemistry to highlight an essential role for AhR in microglial phagocytic function and suggests the therapeutic potential of AhR in demyelinating diseases.

Sat Mar 25 00:00:00 EDT 2023

Microglial aryl hydrocarbon receptor enhances phagocytic function via SYK and promotes remyelination in the cuprizone mouse model of demyelination.

Journal of neuroinflammation

Wang Y,Sun J,Zhu K,Wang D,Zhao X,Zhang H,Wu S,Wang Y,Wang J

MA1-513 was used in Immunohistochemistry to highlight an essential role for AhR in microglial phagocytic function and suggests the therapeutic potential of AhR in demyelinating diseases.

Sat Mar 25 00:00:00 EDT 2023

The Aryl Hydrocarbon Receptor Ligand 6-Formylindolo(3,2-b)carbazole Promotes Estrogen Receptor Alpha and c-Fos Protein Degradation and Inhibits MCF-7 Cell Proliferation and Migration.

Pharmacology

Cano-Sánchez J,Murillo-González FE,de Jesús-Aguilar J,Cabañas-Cortés MA,Tirado-Garibay AC,Elizondo G

Published figure using AHR monoclonal antibody (Product # MA1-513) in Western Blot

Tue Mar 07 00:00:00 EST 2023

Periodontitis salivary microbiota exacerbates nonalcoholic fatty liver disease in high-fat diet-induced obese mice.

iScience

Wang M,Li L,Qian J,Wang N,Bao J,Lu J,Chen F,Li Y,Zhang Y,Yan F

Published figure using AHR monoclonal antibody (Product # MA1-513) in Immunohistochemistry

Fri Apr 21 00:00:00 EDT 2023

Aryl hydrocarbon receptor maintains hepatic mitochondrial homeostasis in mice.

Molecular metabolism

Heo MJ,Suh JH,Lee SH,Poulsen KL,An YA,Moorthy B,Hartig SM,Moore DD,Kim KH

MA1-513 was used in Chromatin immunoprecipitation to provide new insight into how endogenous AhR governs hepatic mitochondrial homeostasis.

Thu Jun 01 00:00:00 EDT 2023

Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy.

The Journal of clinical investigation

van de Veerdonk FL,Renga G,Pariano M,Bellet MM,Servillo G,Fallarino F,De Luca A,Iannitti RG,Piobbico D,Gargaro M,Manni G,D'Onofrio F,Stincardini C,Sforna L,Borghi M,Castelli M,Pieroni S,Oikonomou V,Villella VR,Puccetti M,Giovagnoli S,Galarini R,Barola C,Maiuri L,Della Fazia MA,Cellini B,Talesa VN,Dinarello CA,Costantini C,Romani L

Published figure using AHR monoclonal antibody (Product # MA1-513) in Immunoprecipitation

Tue Jan 18 00:00:00 EST 2022

Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy.

The Journal of clinical investigation

Published figure using AHR monoclonal antibody (Product # MA1-513) in Immunoprecipitation

Tue Jan 18 00:00:00 EST 2022

Intestinal Stem Cells Damaged by Deoxycholic Acid via AHR Pathway Contributes to Mucosal Barrier Dysfunction in High-Fat Feeding Mice.

International journal of molecular sciences

Liu L,Xu J,Xu X,Mao T,Niu W,Wu X,Lu L,Zhou H

MA1-513 was used in Immunohistochemistry to demonstrate for the first time that DCA disrupts intestinal mucosal barrier function by interfering with AHR signalling in ISCs.

Thu Dec 08 00:00:00 EST 2022

LINC00665 knockdown confers sensitivity in irradiated non-small cell lung cancer cells through the miR-582-5p/UCHL3/AhR axis.

Journal of translational medicine

Xu LM,Yuan YJ,Yu H,Wang S,Wang P

MA1-513 was used in Immunohistochemistry and Western Blotting to find that UCHL3 overexpression occurred in both NSCLC tissues and cells, associated with poor prognosis in NSCLC patients.

Tue Aug 02 00:00:00 EDT 2022

LINC00665 knockdown confers sensitivity in irradiated non-small cell lung cancer cells through the miR-582-5p/UCHL3/AhR axis.

Journal of translational medicine

Xu LM,Yuan YJ,Yu H,Wang S,Wang P

MA1-513 was used in Immunohistochemistry and Western Blotting to find that UCHL3 overexpression occurred in both NSCLC tissues and cells, associated with poor prognosis in NSCLC patients.

Tue Aug 02 00:00:00 EDT 2022

IL4I1 and tryptophan metabolites enhance AHR signals to facilitate colorectal cancer progression and immunosuppression.

American journal of translational research

Sun H,Han W,Wen J,Ma X

MA1-513 was used in ChIP Assay, Flow Cytometry, Gel Shift, Immunocytochemistry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot to demonstrate that IL4I1 facilitates colorectal cancer progression and immunosuppression through tryptophan metabolism dependent on AHR activation.

Thu Dec 22 00:00:00 EST 2022

IL4I1 and tryptophan metabolites enhance AHR signals to facilitate colorectal cancer progression and immunosuppression.

American journal of translational research

Sun H,Han W,Wen J,Ma X

Thu Dec 22 00:00:00 EST 2022

Induction of the hepatic aryl hydrocarbon receptor by alcohol dysregulates autophagy and phospholipid metabolism via PPP2R2D.

Nature communications

Kim YS,Ko B,Kim DJ,Tak J,Han CY,Cho JY,Kim W,Kim SG

MA1-513 was used in ChIP Assay, Flow Cytometry, Gel Shift, Immunocytochemistry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot to reveal that AhR induction by alcohol inhibits autophagy in hepatocytes through AMPKα, which is mediated by Ppp2r2d gene transactivation, revealing an AhR-dependent metabolism of phospho-/sphingo-lipids.

Fri Oct 14 00:00:00 EDT 2022

Bioengineering of Genetically Encoded Gene Promoter Repressed by the Flavonoid Apigenin for Constructing Intracellular Sensor for Molecular Events.

Biosensors

Desmet NM,Dhusia K,Qi W,Doseff AI,Bhattacharya S,Gilad AA

MA1-513 was used in Western Blotting to present here show the successful creation of a synthetic gene off" switch that can be monitored directly using an optical reporter gene."

Wed Apr 28 00:00:00 EDT 2021

Bioengineering of Genetically Encoded Gene Promoter Repressed by the Flavonoid Apigenin for Constructing Intracellular Sensor for Molecular Events.

Biosensors

Desmet NM,Dhusia K,Qi W,Doseff AI,Bhattacharya S,Gilad AA

MA1-513 was used in Western Blotting to present here show the successful creation of a synthetic gene off" switch that can be monitored directly using an optical reporter gene."

Wed Apr 28 00:00:00 EDT 2021

AhR activation attenuates calcium oxalate nephrocalcinosis by diminishing M1 macrophage polarization and promoting M2 macrophage polarization.

Theranostics

Yang X,Liu H,Ye T,Duan C,Lv P,Wu X,Liu J,Jiang K,Lu H,Yang H,Xia D,Peng E,Chen Z,Tang K,Ye Z

MA1-513 was used in Chromatin immunoprecipitation to indicate that AhR activation could diminish M1 macrophage polarization and promote M2 macrophage polarization to suppress CaOx nephrocalcinosis via the AhR-miR-142a-IRF1/HIF-1α pathway.

Tue Jun 01 00:00:00 EDT 2021

AhR activation attenuates calcium oxalate nephrocalcinosis by diminishing M1 macrophage polarization and promoting M2 macrophage polarization.

Theranostics

Yang X,Liu H,Ye T,Duan C,Lv P,Wu X,Liu J,Jiang K,Lu H,Yang H,Xia D,Peng E,Chen Z,Tang K,Ye Z

Tue Jun 01 00:00:00 EDT 2021

Immunoregulatory effects of indole-3-carbinol on monocyte-derived macrophages in systemic lupus erythematosus: A crucial role for aryl hydrocarbon receptor.

Autoimmunity

Mohammadi S,Memarian A,Sedighi S,Behnampour N,Yazdani Y

Published figure using AHR monoclonal antibody (Product # MA1-513) in Immunocytochemistry

Wed Aug 01 00:00:00 EDT 2018

Quaking Deficiency Amplifies Inflammation in Experimental Endotoxemia via the Aryl Hydrocarbon Receptor/Signal Transducer and Activator of Transcription 1-NF-κB Pathway.

Frontiers in immunology

Wang L,Zhai DS,Ruan BJ,Xu CM,Ye ZC,Lu HY,Jiang YH,Wang ZY,Xiang A,Yang Y,Yuan JL,Lu ZF

MA1-513 was used in Chromatin immunoprecipitation to suggest a role for QKI in restraining overt innate immune responses by regulating the Ahr/STAT1-NF-κB pathway.

Wed Nov 20 00:00:00 EST 2019

The activation mechanism of the aryl hydrocarbon receptor (AhR) by molecular chaperone HSP90.

FEBS open bio

Tsuji N,Fukuda K,Nagata Y,Okada H,Haga A,Hatakeyama S,Yoshida S,Okamoto T,Hosaka M,Sekine K,Ohtaka K,Yamamoto S,Otaka M,Grave E,Itoh H

Published figure using AHR monoclonal antibody (Product # MA1-513) in Western Blot

Tue Oct 28 00:00:00 EDT 2014

The activation mechanism of the aryl hydrocarbon receptor (AhR) by molecular chaperone HSP90.

FEBS open bio

Tsuji N,Fukuda K,Nagata Y,Okada H,Haga A,Hatakeyama S,Yoshida S,Okamoto T,Hosaka M,Sekine K,Ohtaka K,Yamamoto S,Otaka M,Grave E,Itoh H

Published figure using AHR monoclonal antibody (Product # MA1-513) in Western Blot

Tue Oct 28 00:00:00 EDT 2014

The activation mechanism of the aryl hydrocarbon receptor (AhR) by molecular chaperone HSP90.

FEBS open bio

Tsuji N,Fukuda K,Nagata Y,Okada H,Haga A,Hatakeyama S,Yoshida S,Okamoto T,Hosaka M,Sekine K,Ohtaka K,Yamamoto S,Otaka M,Grave E,Itoh H

Published figure using AHR monoclonal antibody (Product # MA1-513) in Western Blot

Tue Oct 28 00:00:00 EDT 2014

Tobacco smoke-induced skin pigmentation is mediated by the aryl hydrocarbon receptor.

Experimental dermatology

Nakamura M,Ueda Y,Hayashi M,Kato H,Furuhashi T,Morita A

MA1-513 was used in immunocytochemistry to study the role of the aryl hydrocarbon receptor in the mechanism by which tobacco smoke induces skin pigmentation

Thu Aug 01 00:00:00 EDT 2013

Effect of transient TCDD exposure on immortalized human trophoblast-derived cell lines.

Human & experimental toxicology

Fukushima K,Tsukimori K,Li D,Takao T,Morokuma S,Kato K,Seki H,Takeda S,Matsumura S,Wake N

MA1-513 was used in western blot to investigate the influence of TCDD exposure on human extravillous cytotrophoblast lines

Fri Jun 01 00:00:00 EDT 2012

Aryl hydrocarbon receptor-mediated up-regulation of ATP-driven xenobiotic efflux transporters at the blood-brain barrier.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Wang X,Hawkins BT,Miller DS

MA1-513 was used in EMSA to study the role of aryl hydrocarbon receptor in the regulation of ATP-driven xenobiotic efflux transporters at the blood-brain barrier

Tue Feb 01 00:00:00 EST 2011

A role of the aryl hydrocarbon receptor in attenuation of colitis.

Digestive diseases and sciences

Furumatsu K,Nishiumi S,Kawano Y,Ooi M,Yoshie T,Shiomi Y,Kutsumi H,Ashida H,Fujii-Kuriyama Y,Azuma T,Yoshida M

MA1-513 was used in immunohistochemistry to investigate the importance of aryl hydrocarbon receptor in intestinal immunity

Thu Sep 01 00:00:00 EDT 2011

A 5'-region polymorphism modulates promoter activity of the tumor suppressor gene MFSD2A.

Molecular cancer

Colombo F,Falvella FS,Galvan A,Frullanti E,Kunitoh H,Ushijima T,Dragani TA

MA1-513 was used in immunoprecipitation to study the polymorphisms of MFSD2A gene and their effects on its promoter activity

Thu Jul 07 00:00:00 EDT 2011

Ube2l3 gene expression is modulated by activation of the aryl hydrocarbon receptor: implications for p53 ubiquitination.

Biochemical pharmacology

Reyes-Hernández OD,Mejía-García A,Sánchez-Ocampo EM,Cabañas-Cortés MA,Ramírez P,Chávez-González L,Gonzalez FJ,Elizondo G

MA1-513 was used in ChIP assay to investigate the role of aryl hydrocarbon receptor in p53 ubiquitination and Ube2l3 gene expression

Wed Sep 15 00:00:00 EDT 2010

Cytochromes P4501 (CYP1): catalytic activities and inducibility by diesel exhaust particle extract and benzo[a]pyrene in intact human lung ex vivo.

Toxicology

Iba MM,Shin M,Caccavale RJ

MA1-513 was used in western blot to investigate cytochromes P450 activity and its regulation by diesel exhaust particle extract and benzo[a]pyrene in intact human lung

Tue Jun 29 00:00:00 EDT 2010

Aryl hydrocarbon receptor is a transcriptional activator of the human breast cancer resistance protein (BCRP/ABCG2).

Molecular pharmacology

Tan KP,Wang B,Yang M,Boutros PC,Macaulay J,Xu H,Chuang AI,Kosuge K,Yamamoto M,Takahashi S,Wu AM,Ross DD,Harper PA,Ito S

MA1-513 was used in ChIP assay to study the role of aryl hydrocarbon receptors in regulation of breast cancer resistance

Sun Aug 01 00:00:00 EDT 2010

Aryl hydrocarbon receptor functions as a potent coactivator of E2F1-dependent trascription activity.

Biological & pharmaceutical bulletin

Watabe Y,Nazuka N,Tezuka M,Shimba S

MA1-513 was used in western blot to investigate the role of aryl hydrocarbon receptor in gene transcription

Mon Sep 20 00:00:00 EDT 2010

2,3,7,8-tetrachlorodibenzo-p-dioxin impairs an insulin signaling pathway through the induction of tumor necrosis factor-alpha in adipocytes.

Toxicological sciences : an official journal of the Society of Toxicology

Nishiumi S,Yoshida M,Azuma T,Yoshida K,Ashida H

MA1-513 was used in western blot to investigate the effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin on insulin signaling pathway in adipocytes

Tue Jun 01 00:00:00 EDT 2010

Role of GAC63 in transcriptional activation mediated by the aryl hydrocarbon receptor.

The Journal of biological chemistry

Chen YH,Beischlag TV,Kim JH,Perdew GH,Stallcup MR

MA1-513 was used in western blot and chromatin immunoprecipitation to investigate the function of GAC63 in AHR-mediated transcriptional activation.

Fri May 05 00:00:00 EDT 2006

Role of GAC63 in transcriptional activation mediated by the aryl hydrocarbon receptor.

The Journal of biological chemistry

Chen YH,Beischlag TV,Kim JH,Perdew GH,Stallcup MR

MA1-513 was used in western blot and chromatin immunoprecipitation to investigate the function of GAC63 in AHR-mediated transcriptional activation.

Fri May 05 00:00:00 EDT 2006

2,3,7,8-Tetrachlorodibenzo-p-dioxin induces insulin-like growth factor binding protein-1 gene expression and counteracts the negative effect of insulin.

Molecular pharmacology

Marchand A,Tomkiewicz C,Marchandeau JP,Boitier E,Barouki R,Garlatti M

MA1-513 was used in EMSA assay to investigate the effect of 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) on insulin-like growth factor binding protein-1 (IGFBP-1) expression

Tue Feb 01 00:00:00 EST 2005

Dietary polyphenols increase paraoxonase 1 gene expression by an aryl hydrocarbon receptor-dependent mechanism.

Molecular and cellular biology

Gouédard C,Barouki R,Morel Y

MA1-513 was used in EMSA to investigate the role of dietary polyphenols in paraoxonase 1 gene expression.

Tue Jun 01 00:00:00 EDT 2004

A new southwestern chemistry-based ELISA for detection of aryl hydrocarbon receptor transformation: application to the screening of its receptor agonists and antagonists.

Journal of immunological methods

Fukuda I,Nishiumi S,Yabushita Y,Mukai R,Kodoi R,Hashizume K,Mizuno M,Hatanaka Y,Ashida H

MA1-513 was used in ELISA to evaluate a novel ELISA strategy for the screening of aryl hydrocarbon receptor activators and inhibitors

Thu Apr 01 00:00:00 EST 2004

Role of the coiled-coil coactivator (CoCoA) in aryl hydrocarbon receptor-mediated transcription.

The Journal of biological chemistry

Kim JH,Stallcup MR

MA1-513 was used in chromatin immunoprecipitation to investigate the role of the coiled-coil coactivator (CoCoA) during the AHR/ARNT-mediated transcription.

Fri Nov 26 00:00:00 EST 2004

Role of the coiled-coil coactivator (CoCoA) in aryl hydrocarbon receptor-mediated transcription.

The Journal of biological chemistry

Kim JH,Stallcup MR

MA1-513 was used in chromatin immunoprecipitation to investigate the role of the coiled-coil coactivator (CoCoA) during the AHR/ARNT-mediated transcription.

Fri Nov 26 00:00:00 EST 2004

7-ketocholesterol is an endogenous modulator for the arylhydrocarbon receptor.

The Journal of biological chemistry

Savouret JF,Antenos M,Quesne M,Xu J,Milgrom E,Casper RF

MA1-513 was used in western blot to identify 7-ketocholesterol as an endogenous modulator that inhibits transactivation by the arylhydrocarbon receptor through competitive binding against xenobiotic ligands.

Fri Feb 02 00:00:00 EST 2001

Characterization of the AhR-hsp90-XAP2 core complex and the role of the immunophilin-related protein XAP2 in AhR stabilization.

Biochemistry

Meyer BK,Perdew GH

MA1-513 was used in ELISA to investigate the function of XAP2 for the regulation of AhR stabilization

Tue Jul 13 00:00:00 EDT 1999

Bioinformatics

Protein Aliases: Ah receptor; AH-receptor; aromatic hydrocarbon receptor; Aryl hydrocarbon receptor; bHLHe76; Class E basic helix-loop-helix protein 76; dioxin receptor

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Gene Aliases: Ah; Ahh; AHR; Ahre; BHLHE76; In

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UniProt ID: (Human) P35869, (Mouse) P30561

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Entrez Gene ID: (Human) 196, (Mouse) 11622, (Rat) 25690

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Function(s)

DNA binding transcription factor activity, sequence-specific DNA binding aryl hydrocarbon receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding transcription factor binding enhancer binding transcription regulatory region DNA binding protein heterodimerization activity protein dimerization activity Hsp90 protein binding E-box binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding signal transducer activity sequence-specific DNA binding basic helix-loop-helix transcription factor

Process(es)

ovarian follicle development blood vessel development regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter xenobiotic metabolic process apoptotic process cell cycle response to xenobiotic stimulus response to toxic substance regulation of gene expression intracellular receptor signaling pathway regulation of B cell proliferation response to estradiol circadian regulation of gene expression negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cellular response to glucocorticoid stimulus negative regulation of transcription from RNA polymerase II promoter cell morphogenesis patterning of blood vessels B cell homeostasis liver development B-1 B cell homeostasis blood vessel remodeling lymphocyte homeostasis immune system process negative regulation of systemic arterial blood pressure cardiac left ventricle morphogenesis circumferential growth involved in left ventricle morphogenesis transcription, DNA-templated response to stress signal transduction blood circulation regulation of blood pressure response to organic cyclic compound B cell differentiation prostate gland development embryonic hemopoiesis post-embryonic hemopoiesis camera-type eye development T cell homeostasis positive regulation of cell size positive regulation of RNA polymerase II transcriptional preinitiation complex assembly negative regulation of vasoconstriction positive regulation of growth rhythmic process blood vessel morphogenesis spleen development reproductive structure development gland development smooth muscle tissue development regulation of blood vessel size regulation of heart growth negative regulation of necrotic cell death venous blood vessel development kidney morphogenesis common bile duct development cellular response to cAMP glomerulus morphogenesis

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AHR Monoclonal Antibody (RPT9)

AHR Antibody (MA1-513) in ICC/IF

Product Details

MA1-513

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Gel Shift (GS)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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