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Invitrogen
AIF Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
Product Details
PA5-29693
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 82 and 330 of Human AIF
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547167
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse liver, rat liver.
Predicted reactivity: Mouse (95%), Rat (96%), Zebrafish (80%), Pig (97%), Chicken (84%), Rhesus Monkey (99%), Bovine (95%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF was recently identified, and cloned. Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants of AIF and a related pseudogene has been identified on chromosome 10.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AGR1; Apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated, 1; ERP18; ERP19; hAG-1; harlequin; MGC111425; pcd8; PDIA16; programmed cell death 8 (apoptosis-inducing factor); Programmed cell death protein 8; RP3-438D16.2; striatal apoptosis-inducing factor; testicular secretory protein Li 4; TLP19; UNQ713/PRO1376
Gene Aliases: AIF; AIFM1; AIFsh2; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; Hq; NADMR; NAMSD; PDCD8
UniProt ID: (Human) O95831, (Mouse) Q9Z0X1, (Rat) Q9JM53
Entrez Gene ID: (Human) 9131, (Mouse) 26926, (Rat) 83533
DNA binding
protein binding
electron carrier activity
NAD(P)H oxidase activity
oxidoreductase activity, acting on NAD(P)H
protein dimerization activity
FAD binding
C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity
aldo-keto reductase (NADP) activity
electron-transferring-flavoprotein dehydrogenase activity
isocitrate dehydrogenase activity
mevaldate reductase activity
gluconate dehydrogenase activity
steroid dehydrogenase activity
oxidoreductase activity
epoxide dehydrogenase activity
5-exo-hydroxycamphor dehydrogenase activity
2-hydroxytetrahydrofuran dehydrogenase activity
acetoin dehydrogenase activity
phenylcoumaran benzylic ether reductase activity
D-xylose:NADP reductase activity
L-arabinose:NADP reductase activity
D-arabinitol dehydrogenase, D-ribulose forming (NADP+) activity
steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
steroid dehydrogenase activity, acting on the CH-CH group of donors
(R)-(-)-1,2,3,4-tetrahydronaphthol dehydrogenase activity
3-hydroxymenthone dehydrogenase activity
very long-chain-3-hydroxyacyl-CoA dehydrogenase activity
dihydrotestosterone 17-beta-dehydrogenase activity
(R)-2-hydroxyisocaproate dehydrogenase activity
L-arabinose 1-dehydrogenase (NADP+) activity
L-xylulose reductase (NAD+) activity
3-ketoglucose-reductase activity
flavin adenine dinucleotide binding
(R)-2-hydroxyglutarate dehydrogenase activity
D-arabinitol dehydrogenase, D-xylulose forming (NADP+) activity
oxidoreductase
response to ischemia
DNA catabolic process
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
response to toxic substance
chromosome condensation
positive regulation of apoptotic process
positive regulation of neuron apoptotic process
cell redox homeostasis
neuron apoptotic process
oxidation-reduction process
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to hydrogen peroxide
cellular response to estradiol stimulus
cellular response to nitric oxide
cellular response to oxygen-glucose deprivation
response to L-glutamate
regulation of apoptotic DNA fragmentation
cellular response to aldosterone
response to oxidative stress
apoptotic mitochondrial changes
positive regulation of cell death
neuron differentiation
mitochondrial respiratory chain complex I assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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