Invitrogen

AIF Monoclonal Antibody (4E7E11)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

9 Published Figures

11 References

View all (37) AIF antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

AIF Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

AIF Antibody (MA5-15880) in ICC/IF

Immunofluorescent analysis of Cytochrome C (red) in HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes at -20°C, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a Cytochrome C monoclonal antibody, Alexa Fluor 647 conjugate (Product # MA3-38200-A647) at a dilution of 10 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Cells were then stained with a AIF mouse monoclonal antibody (Product # MA5-15880) at ... View More

AIF Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

AIF Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

AIF Antibody in Immunohistochemistry (IHC)

AIF Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

AIF Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in AIF knockout (KO) cell line using Anti-AIF Monoclonal Antibody (4E7) (Product # MA5-15880). {KO}

Product Details

MA5-15880

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:50-1:100

View 3 publications 3 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

4E7E11

Immunogen

Purified recombinant fragment of human AIF expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153781

Product Specific Information

MA5-15880 targets AIF in FACS, IF, IHC, and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.

The MA5-15880 immunogen is purified recombinant fragment of human AIF expressed in E. Coli. .

MA5-15880 detects AIF which has a predicted molecular weight of approximately 67kDa.

Target Information

Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF was recently identified, and cloned. Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants of AIF and a related pseudogene has been identified on chromosome 10.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Novel dicarbonyl metabolic pathway via mitochondrial ES1 possessing glyoxalase III activity.

BBA advances

Ito G,Tatara Y,Itoh K,Yamada M,Yamashita T,Sakamoto K,Nozaki T,Ishida K,Wake Y,Kaneko T,Fukuda T,Sugano E,Tomita H,Ozaki T

MA5-15880 was used in Western Blotting to show a novel metabolic pathway for reactive dicarbonyls in mitochondria.

Wed May 31 00:00:00 EDT 2023

Distinct mechanism of cervical cancer cell death caused by the investigational new drug SHetA2.

Frontiers in oncology

Rai R,Chandra V,Kennedy AL,Zuna RE,Benbrook DM

MA5-15880 was used in Immunocytochemistry to evaluate the roles of mitochondrial damage and another SHetA2-target HSP protein, cytoplasmic heat shock cognate 70 (hsc70), in the mechanism of SHetA2 killing of cervical cancer cells.

Thu Feb 09 00:00:00 EST 2023

Distinct mechanism of cervical cancer cell death caused by the investigational new drug SHetA2.

Frontiers in oncology

Rai R,Chandra V,Kennedy AL,Zuna RE,Benbrook DM

Thu Feb 09 00:00:00 EST 2023

Utility and Mechanism of SHetA2 and Paclitaxel for Treatment of Endometrial Cancer.

Cancers

Chandra V,Rai R,Benbrook DM

Published figure using AIF monoclonal antibody (Product # MA5-15880) in Western Blot

Wed May 12 00:00:00 EDT 2021

Apoptosis-Inducing Factor, Protein Expression, and Apoptosis Changes with Glutamine in Podocytes Cells Exposed with Cisplatin.

Veterinary medicine international

Susilo I,Maulida H,Alimsardjono L,Fauziah D,Pertiwi H

MA5-15880 was used in Immunohistochemistry to investigate the protective effect of glutamine on cisplatin nephrotoxicity in the kidney.

Thu May 13 00:00:00 EDT 2021

Apoptosis-Inducing Factor, Protein Expression, and Apoptosis Changes with Glutamine in Podocytes Cells Exposed with Cisplatin.

Veterinary medicine international

Susilo I,Maulida H,Alimsardjono L,Fauziah D,Pertiwi H

MA5-15880 was used in Immunohistochemistry to investigate the protective effect of glutamine on cisplatin nephrotoxicity in the kidney.

Thu May 13 00:00:00 EDT 2021

Decreased expression of apoptosis-inducing factor in renal cell carcinoma is associated with poor prognosis and reduced postoperative survival.

Oncology letters

Wang Z,Yuan C,Huang Y,Liu Z,Yu X,Lv C,Su Z

Published figure using AIF monoclonal antibody (Product # MA5-15880) in Immunofluorescence

Sun Sep 01 00:00:00 EDT 2019

Decreased expression of apoptosis-inducing factor in renal cell carcinoma is associated with poor prognosis and reduced postoperative survival.

Oncology letters

Wang Z,Yuan C,Huang Y,Liu Z,Yu X,Lv C,Su Z

Published figure using AIF monoclonal antibody (Product # MA5-15880) in Immunofluorescence

Sun Sep 01 00:00:00 EDT 2019

New protein-protein interactions of mitochondrial connexin 43 in mouse heart.

Journal of cellular and molecular medicine

Denuc A,Núñez E,Calvo E,Loureiro M,Miro-Casas E,Guarás A,Vázquez J,Garcia-Dorado D

MA5-15880 was used in immunocytochemistry, immunoprecipitation, proximity ligation assay, and western blot to discover mitochondrial connexin 43 protein-protein interactions in mouse heart

Sun May 01 00:00:00 EDT 2016

New protein-protein interactions of mitochondrial connexin 43 in mouse heart.

Journal of cellular and molecular medicine

Denuc A,Núñez E,Calvo E,Loureiro M,Miro-Casas E,Guarás A,Vázquez J,Garcia-Dorado D

MA5-15880 was used in immunocytochemistry, immunoprecipitation, proximity ligation assay, and western blot to discover mitochondrial connexin 43 protein-protein interactions in mouse heart

Sun May 01 00:00:00 EDT 2016

New protein-protein interactions of mitochondrial connexin 43 in mouse heart.

Journal of cellular and molecular medicine

Denuc A,Núñez E,Calvo E,Loureiro M,Miro-Casas E,Guarás A,Vázquez J,Garcia-Dorado D

MA5-15880 was used in immunocytochemistry, immunoprecipitation, proximity ligation assay, and western blot to discover mitochondrial connexin 43 protein-protein interactions in mouse heart

Sun May 01 00:00:00 EDT 2016

From ventriculomegaly to severe muscular atrophy: expansion of the clinical spectrum related to mutations in AIFM1.

Mitochondrion

Kettwig M,Schubach M,Zimmermann FA,Klinge L,Mayr JA,Biskup S,Sperl W,Gärtner J,Huppke P

MA5-15880 was used in western blot to discuss the known phenotypes related to AIFM1 mutations and further study a single patient

Sun Mar 01 00:00:00 EST 2015

Bioinformatics

Protein Aliases: AGR1; Apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated, 1; ERP18; ERP19; hAG-1; harlequin; MGC111425; pcd8; PDIA16; programmed cell death 8 (apoptosis-inducing factor); Programmed cell death protein 8; RP3-438D16.2; striatal apoptosis-inducing factor; testicular secretory protein Li 4; TLP19; UNQ713/PRO1376

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Gene Aliases: AIF; AIFM1; AIFsh2; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; Hq; NADMR; NAMSD; PDCD8

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UniProt ID: (Human) O95831, (Mouse) Q9Z0X1, (Rat) Q9JM53

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Entrez Gene ID: (Human) 9131, (Mouse) 26926, (Rat) 83533

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Function(s)

Process(es)

response to ischemia DNA catabolic process apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process response to toxic substance chromosome condensation positive regulation of apoptotic process positive regulation of neuron apoptotic process cell redox homeostasis neuron apoptotic process oxidation-reduction process intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to hydrogen peroxide cellular response to estradiol stimulus cellular response to nitric oxide cellular response to oxygen-glucose deprivation response to L-glutamate regulation of apoptotic DNA fragmentation cellular response to aldosterone response to oxidative stress apoptotic mitochondrial changes positive regulation of cell death neuron differentiation mitochondrial respiratory chain complex I assembly

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AIF Monoclonal Antibody (4E7E11)

AIF Antibody (MA5-15880) in ICC/IF

Product Details

MA5-15880

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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