Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 4
AKT2 Antibody (PA5-13760) in ICC/IF
Immunocytochemistry analysis of AKT2 in C2C12 cells. Samples were incubated with AKT2 polyclonal antibody (Product # PA5-13760) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min). AKT2 i... View More
Product Details
PA5-13760
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 416-444 amino acids from the C-terminal region of human AKT2
Conjugate
Form
Liquid
Purification
Ammonium sulfate precipitation, Size-exclusion - Dialysis
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2242520
Product Specific Information
This antibody is predicted to react with mouse and rat based on sequence homology.
Target Information
AKT2 is a phosphoinositide-dependent serine/threonine protein kinase that is enriched in insulin responsive tissues and has been implicated in the metabolic actions of the hormone. Akt is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphatidylinositol 3-kinase (PI 3-kinase). Akt2 is amplified and overexpressed in some human cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: murine thymoma viral (v-akt) homolog-2; PKB beta; Protein kinase Akt-2; Protein kinase B beta; protein kinase B, beta; putative v-akt murine thymoma viral oncoprotein 2; RAC protein kinase beta; RAC-beta serine/threonine protein kinase; RAC-beta serine/threonine-protein kinase; RAC-PK-beta; v-akt murine thymoma viral oncogene homolog 2
Gene Aliases: 2410016A19Rik; AKT2; AW554154; HIHGHH; PKB; PKBB; PKBBETA; PRKBB; RAC-BETA
UniProt ID: (Human) Q0VAN1, (Mouse) Q60823
Entrez Gene ID: (Human) 208, (Mouse) 11652
protein serine/threonine kinase activity
protein kinase C binding
protein binding
ATP binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
protein modifying enzyme
positive regulation of protein phosphorylation
glycogen biosynthetic process
glucose metabolic process
regulation of translation
cellular protein modification process
apoptotic process
response to osmotic stress
signal transduction
insulin receptor signaling pathway
carbohydrate transport
positive regulation of signal transduction
negative regulation of plasma membrane long-chain fatty acid transport
positive regulation of glucose metabolic process
response to muscle activity
peptidyl-serine phosphorylation
regulation of cell migration
positive regulation of cell migration
positive regulation of vesicle fusion
response to nutrient levels
positive regulation of fatty acid beta-oxidation
peripheral nervous system myelin maintenance
cellular response to insulin stimulus
negative regulation of RNA splicing
positive regulation of peptidyl-serine phosphorylation
intracellular signal transduction
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
protein kinase B signaling
positive regulation of nitric oxide biosynthetic process
fat cell differentiation
positive regulation of glycogen biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
positive regulation of glucose import
positive regulation of positive chemotaxis
mammary gland epithelial cell differentiation
intracellular protein transmembrane transport
regulation of cell cycle arrest
cellular response to organic cyclic compound
protein localization to plasma membrane
positive regulation of protein targeting to membrane
activation of GTPase activity
positive regulation of cell motility
positive regulation of glucose import in response to insulin stimulus
carbohydrate metabolic process
glycogen metabolic process
protein phosphorylation
transport
multicellular organism development
positive regulation of gene expression
positive regulation of sodium ion transport
phosphorylation
negative regulation of apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support