Invitrogen

ALK Monoclonal Antibody (4C5B8)

Advanced Verification

IP-mass spectrometry

13 Published Figures

16 References

View all (166) ALK antibodies

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ALK Antibody (35-4300)

IP-MS enrichment of ALK (LFQ intensity): ALK was enriched 416-fold from SR lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and ALK antibody (Product # 35-4300). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selec... View More

ALK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ALK Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

ALK Antibody in Western Blot, Immunocytochemistry, Immunomicroscopy (WB, ICC/IF, IM)

ALK Antibody in Immunocytochemistry (ICC/IF)

ALK Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

ALK Antibody — IP-MS enrichment of ALK (LFQ intensity): ALK was enriched 416-fold from SR lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and ALK antibody (Product # 35-4300). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. {IP-MS}

Product Details

35-4300

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

Immunomicroscopy (IM)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4C5B8

Immunogen

Recombinant protein derived from the human ALK protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533203

Target Information

Anaplastic Lymphoma Receptor Tyrosine Kinase (ALK) belongs to the insulin receptor superfamily. It is vital for brain development. Mutations, rearrangements, and amplifications in the ALK gene have been found in tumors, including anaplastic large cell lymphoma, neurblastoma, and non-small cell lung cancer. Chromosomal rearrangement is the most common genetic alteration. The translocation creates a fusion gene consisting of the ALK gene and the nucleophosmin gene: the 3' half of ALK, derived from chromosome 2, is fused to the 5' portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK. ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Extracellular domain shedding of the ALK receptor mediates neuroblastoma cell migration.

Cell reports

Huang H,Gont A,Kee L,Dries R,Pfeifer K,Sharma B,Debruyne DN,Harlow M,Sengupta S,Guan J,Yeung CM,Wang W,Hallberg B,Palmer RH,Irwin MS,George RE

35-4300 was used in Western Blotting to indicate a pivotal role for WT ALK extracellular domain cleavage in NB pathogenesis, which may be harnessed for therapeutic benefit.

Tue Jul 13 00:00:00 EDT 2021

Wiskott-Aldrich syndrome protein (WASP) is a tumor suppressor in T cell lymphoma.

Nature medicine

Menotti M,Ambrogio C,Cheong TC,Pighi C,Mota I,Cassel SH,Compagno M,Wang Q,Dall'Olio R,Minero VG,Poggio T,Sharma GG,Patrucco E,Mastini C,Choudhari R,Pich A,Zamo A,Piva R,Giliani S,Mologni L,Collings CK,Kadoch C,Gambacorti-Passerini C,Notarangelo LD,Anton IM,Voena C,Chiarle R

35-4300 was used in Western Blotting to study the role of the WIskott-Aldrich Syndrome protein and WASP interacting protein in lymphoma.

Tue Jan 01 00:00:00 EST 2019

Excess of NPM-ALK oncogenic signaling promotes cellular apoptosis and drug dependency.

Oncogene

Ceccon M,Merlo MEB,Mologni L,Poggio T,Varesio LM,Menotti M,Bombelli S,Rigolio R,Manazza AD,Di Giacomo F,Ambrogio C,Giudici G,Casati C,Mastini C,Compagno M,Turner SD,Gambacorti-Passerini C,Chiarle R,Voena C

Published figure using ALK monoclonal antibody (Product # 35-4300) in Immunofluorescence

Thu Jul 21 00:00:00 EDT 2016

Excess of NPM-ALK oncogenic signaling promotes cellular apoptosis and drug dependency.

Oncogene

Published figure using ALK monoclonal antibody (Product # 35-4300) in Immunofluorescence

Thu Jul 21 00:00:00 EDT 2016

Excess of NPM-ALK oncogenic signaling promotes cellular apoptosis and drug dependency.

Oncogene

Published figure using ALK monoclonal antibody (Product # 35-4300) in Immunofluorescence

Thu Jul 21 00:00:00 EDT 2016

Entrectinib, a Pan-TRK, ROS1, and ALK Inhibitor with Activity in Multiple Molecularly Defined Cancer Indications.

Molecular cancer therapeutics

Ardini E,Menichincheri M,Banfi P,Bosotti R,De Ponti C,Pulci R,Ballinari D,Ciomei M,Texido G,Degrassi A,Avanzi N,Amboldi N,Saccardo MB,Casero D,Orsini P,Bandiera T,Mologni L,Anderson D,Wei G,Harris J,Vernier JM,Li G,Felder E,Donati D,Isacchi A,Pesenti E,Magnaghi P,Galvani A

35-4300 was used in western blot to characterize multiple molecularly defined cancer indications by studying ROS1, ALK inhibitor, Entrectinib, a Pan-TRK activity

Fri Apr 01 00:00:00 EDT 2016

Molecular and functional characterizations of the association and interactions between nucleophosmin-anaplastic lymphoma kinase and type I insulin-like growth factor receptor.

Neoplasia (New York, N.Y.)

Shi B,Vishwamitra D,Granda JG,Whitton T,Shi P,Amin HM

Published figure using ALK monoclonal antibody (Product # 35-4300) in Immunoprecipitation

Sat Jun 01 00:00:00 EDT 2013

Molecular and functional characterizations of the association and interactions between nucleophosmin-anaplastic lymphoma kinase and type I insulin-like growth factor receptor.

Neoplasia (New York, N.Y.)

Shi B,Vishwamitra D,Granda JG,Whitton T,Shi P,Amin HM

Published figure using ALK monoclonal antibody (Product # 35-4300) in Immunoprecipitation

Sat Jun 01 00:00:00 EDT 2013

Molecular and functional characterizations of the association and interactions between nucleophosmin-anaplastic lymphoma kinase and type I insulin-like growth factor receptor.

Neoplasia (New York, N.Y.)

Shi B,Vishwamitra D,Granda JG,Whitton T,Shi P,Amin HM

Published figure using ALK monoclonal antibody (Product # 35-4300) in Immunoprecipitation

Sat Jun 01 00:00:00 EDT 2013

Molecular and functional characterizations of the association and interactions between nucleophosmin-anaplastic lymphoma kinase and type I insulin-like growth factor receptor.

Neoplasia (New York, N.Y.)

Shi B,Vishwamitra D,Granda JG,Whitton T,Shi P,Amin HM

Published figure using ALK monoclonal antibody (Product # 35-4300) in Immunoprecipitation

Sat Jun 01 00:00:00 EDT 2013

CEP-28122, a highly potent and selective orally active inhibitor of anaplastic lymphoma kinase with antitumor activity in experimental models of human cancers.

Molecular cancer therapeutics

Cheng M,Quail MR,Gingrich DE,Ott GR,Lu L,Wan W,Albom MS,Angeles TS,Aimone LD,Cristofani F,Machiorlatti R,Abele C,Ator MA,Dorsey BD,Inghirami G,Ruggeri BA

35-4300 was used in western blot to identify and characterize an anaplastic lymphoma kinase inhibitor.

Thu Mar 01 00:00:00 EST 2012

NPM-ALK inhibits the p53 tumor suppressor pathway in an MDM2 and JNK-dependent manner.

Blood

Cui YX,Kerby A,McDuff FK,Ye H,Turner SD

Thu May 21 00:00:00 EDT 2009

NPM-ALK inhibits the p53 tumor suppressor pathway in an MDM2 and JNK-dependent manner.

Blood

Cui YX,Kerby A,McDuff FK,Ye H,Turner SD

Thu May 21 00:00:00 EDT 2009

Ablation of oncogenic ALK is a viable therapeutic approach for anaplastic large-cell lymphomas.

Blood

Piva R,Chiarle R,Manazza AD,Taulli R,Simmons W,Ambrogio C,D'Escamard V,Pellegrino E,Ponzetto C,Palestro G,Inghirami G

35-4300 was used in immunocytochemistry and western blot to examine the consequences of constitutive expression of anaplastic lymphoma kinase

Sun Jan 15 00:00:00 EST 2006

Ablation of oncogenic ALK is a viable therapeutic approach for anaplastic large-cell lymphomas.

Blood

Piva R,Chiarle R,Manazza AD,Taulli R,Simmons W,Ambrogio C,D'Escamard V,Pellegrino E,Ponzetto C,Palestro G,Inghirami G

35-4300 was used in immunocytochemistry and western blot to examine the consequences of constitutive expression of anaplastic lymphoma kinase

Sun Jan 15 00:00:00 EST 2006

Anaplastic lymphoma kinase activity is essential for the proliferation and survival of anaplastic large-cell lymphoma cells.

Blood

Wan W,Albom MS,Lu L,Quail MR,Becknell NC,Weinberg LR,Reddy DR,Holskin BP,Angeles TS,Underiner TL,Meyer SL,Hudkins RL,Dorsey BD,Ator MA,Ruggeri BA,Cheng M

35-4300 was used in Western Blotting to evaluate whether functional ALK is essential for the proliferation and survival of ALK+ anaplastic large-cell lymphoma cells in culture.

Wed Feb 15 00:00:00 EST 2006

Bioinformatics

Protein Aliases: ALK tyrosine kinase receptor; ALK/NPM1 fusion gene; Anaplastic lymphoma kinase; Anaplastic lymphoma kinase Ki1; Anaplastic Lymphoma Kinase p80; CD246; CD246 antigen; EC 2.7.10.1; kinase ALK; mutant anaplastic lymphoma kinase; npm-alk; TFG/ALK

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Gene Aliases: ALK; CD246; NBLST3

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UniProt ID: (Human) Q9UM73

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Entrez Gene ID: (Human) 238

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Function(s)

NF-kappaB-inducing kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity protein binding ATP binding identical protein binding transmembrane signal receptor

Process(es)

activation of MAPK activity signal transduction transmembrane receptor protein tyrosine kinase signaling pathway cell proliferation phosphorylation peptidyl-tyrosine phosphorylation NIK/NF-kappaB signaling regulation of apoptotic process protein autophosphorylation neuron development positive regulation of NF-kappaB transcription factor activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ALK Monoclonal Antibody (4C5B8)

ALK Antibody (35-4300)

Product Details

35-4300

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Immunomicroscopy (IM)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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