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ALK Antibody (MA5-55785) in WB
Western blot analysis of ALK in 15 µg of SH-SY5Y. Sample was run on 6-18% SDS-PAGE under reducing conditions, blotted onto nitrocellulose membrane, and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. ALK band was visualized using ECL Western Blotting Substrate. Incubation with primary ALK monoclonal antibody (Product # MA5-55785) at a dilution of 1 µg/mL was used.
Product Details
MA5-55785
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
K40048_8D10
Immunogen
Recombinant human ALK (immunogen range is 1461-1530).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Dry ice (international)
Product Specific Information
Sequence of this protein is as follows: ISVRVPRGPA VEGGHVNMAF SQSNPPSELH KVHGSRNKPT SLWNPTYGSW FTEKPTKKNN PIAKKEPHDR
Target Information
Anaplastic Lymphoma Receptor Tyrosine Kinase (ALK) belongs to the insulin receptor superfamily. It is vital for brain development. Mutations, rearrangements, and amplifications in the ALK gene have been found in tumors, including anaplastic large cell lymphoma, neurblastoma, and non-small cell lung cancer. Chromosomal rearrangement is the most common genetic alteration. The translocation creates a fusion gene consisting of the ALK gene and the nucleophosmin gene: the 3' half of ALK, derived from chromosome 2, is fused to the 5' portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK. ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ALK tyrosine kinase receptor; ALK/NPM1 fusion gene; Anaplastic lymphoma kinase; Anaplastic lymphoma kinase Ki1; Anaplastic Lymphoma Kinase p80; CD246; CD246 antigen; EC 2.7.10.1; kinase ALK; mutant anaplastic lymphoma kinase; npm-alk; TFG/ALK
Gene Aliases: ALK; CD246; NBLST3
UniProt ID: (Human) Q9UM73
Entrez Gene ID: (Human) 238
activation of MAPK activity
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
cell proliferation
phosphorylation
peptidyl-tyrosine phosphorylation
NIK/NF-kappaB signaling
regulation of apoptotic process
protein autophosphorylation
neuron development
positive regulation of NF-kappaB transcription factor activity
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