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ALK Antibody (MA5-14528) in ICC/IF
Immunofluorescent analysis of ALK (Anaplastic Lymphoma Kinase)/p80 (green) showing staining in the cytoplasm of NCI-H1299 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an ALK/p80 monoclonal antibody (Product # MA5-14528) in 3% BSA-PBS at a dilution of 1:10 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PB... View More
Product Details
MA5-14528
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
SP8
Immunogen
Recombinant fragment within ALK aa 1350-1500. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
tissue culture supernatant/TBS, pH 7.5, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_10982161
Product Specific Information
MA5-14528 targets ALK (Anaplastic Lymphoma Kinase) / p80 in IHC (P) and ICC/IF applications and shows reactivity with Human and Mouse samples.
The MA5-14528 immunogen is recombinant protein corresponding to a region, which spans the tyrosine kinase catalytic domain and part of the C-terminus of the NPM-ALK transcript.
Target Information
Anaplastic Lymphoma Receptor Tyrosine Kinase (ALK) belongs to the insulin receptor superfamily. It is vital for brain development. Mutations, rearrangements, and amplifications in the ALK gene have been found in tumors, including anaplastic large cell lymphoma, neurblastoma, and non-small cell lung cancer. Chromosomal rearrangement is the most common genetic alteration. The translocation creates a fusion gene consisting of the ALK gene and the nucleophosmin gene: the 3' half of ALK, derived from chromosome 2, is fused to the 5' portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK. ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ALK tyrosine kinase receptor; ALK/NPM1 fusion gene; Anaplastic lymphoma kinase; Anaplastic lymphoma kinase Ki1; Anaplastic Lymphoma Kinase p80; CD246; CD246 antigen; EC 2.7.10.1; kinase ALK; mutant anaplastic lymphoma kinase; npm-alk; TFG/ALK
Gene Aliases: ALK; CD246; NBLST3; Tcrz
UniProt ID: (Human) Q9UM73, (Mouse) P97793
Entrez Gene ID: (Human) 238, (Mouse) 11682
NF-kappaB-inducing kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
protein binding
ATP binding
identical protein binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
transmembrane signal receptor
activation of MAPK activity
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
cell proliferation
phosphorylation
peptidyl-tyrosine phosphorylation
NIK/NF-kappaB signaling
regulation of apoptotic process
protein autophosphorylation
neuron development
positive regulation of NF-kappaB transcription factor activity
protein phosphorylation
nervous system development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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