Proteintech

ALKBH5 Polyclonal Antibody

ALKBH5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 11

ALKBH5 Antibody (16837-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 16837-1-AP (ALKBH5 Antibody) at dilution of 1:100 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Product Image Gallery

Product Details

16837-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 81 publications 81 publications

Immunohistochemistry (IHC)

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

View 7 publications 7 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ALKBH5 Fusion Protein Ag10516 (57-383 aa encoded by BC062339)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.47 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: KRKYQEDSD PERSDYEEQQ LQKEEEARKV KSGIRQMRLF SQDECAKIEA RIDEVVSRAE KGLYNEHTVD RAPLRNKYFF GEGYTYGAQL QKRGPGQERL YPPGDVDEIP EWVHQLVIQK LVEHRVIPEG FVNSAVINDY QPGGCIVSHV DPIHIFERPI VSVSFFSDSA LCFGCKFQFK PIRVSEPVLS LPVRRGSVTV LSGYAADEIT HCIRPQDIKE RRAVIILRKT RLDAPRLETK SLSSSVLPPS YASDRLSGNN RDPALKPKRS HRKADPDAAH RPRILEMDKE ENRRSVLLPT HRRRGSFSSE NYWRKSYESS EDCSEAAG (57-383 aa encoded by BC062339 )

Target Information

ALKBH5 (RNA demehtylase ALKBH5) is a dioxygenase that demethylates RNA by oxidative demethylation. Specifically, it demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Demethylation of m6A mRNA affects mRNA processing and export. ALKBH5 can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). It requires molecular oxygen, alpha-ketoglutarate and iron.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Therapeutic m⁶A Eraser ALKBH5 mRNA-Loaded Exosome-Liposome Hybrid Nanoparticles Inhibit Progression of Colorectal Cancer in Preclinical Tumor Models.

ACS nano

Wu S,Yun J,Tang W,Familiari G,Relucenti M,Wu J,Li X,Chen H,Chen R

Tue Jun 27 00:00:00 EDT 2023

Therapeutic m⁶A Eraser ALKBH5 mRNA-Loaded Exosome-Liposome Hybrid Nanoparticles Inhibit Progression of Colorectal Cancer in Preclinical Tumor Models.

ACS nano

Wu S,Yun J,Tang W,Familiari G,Relucenti M,Wu J,Li X,Chen H,Chen R

Tue Jun 27 00:00:00 EDT 2023

Therapeutic m⁶A Eraser ALKBH5 mRNA-Loaded Exosome-Liposome Hybrid Nanoparticles Inhibit Progression of Colorectal Cancer in Preclinical Tumor Models.

ACS nano

Wu S,Yun J,Tang W,Familiari G,Relucenti M,Wu J,Li X,Chen H,Chen R

Tue Jun 27 00:00:00 EDT 2023

ALKBH5 ALLEVIATES HYPOXIA POSTCONDITIONING INJURY IN d -GALACTOSE-INDUCED SENESCENT CARDIOMYOCYTES BY REGULATING STAT3.

Shock (Augusta, Ga.)

Zhang X,Li F,Ma J,Zhang X,Yin Y

Sun Jan 01 00:00:00 EST 2023

Epitranscriptic regulation of HRAS by N⁶-methyladenosine drives tumor progression.

Proceedings of the National Academy of Sciences of the United States of America

Pan Y,Gu Y,Liu T,Zhang Q,Yang F,Duan L,Cheng S,Zhu X,Xi Y,Chang X,Ye Q,Gao S

Tue Apr 04 00:00:00 EDT 2023

Epitranscriptic regulation of HRAS by N⁶-methyladenosine drives tumor progression.

Proceedings of the National Academy of Sciences of the United States of America

Pan Y,Gu Y,Liu T,Zhang Q,Yang F,Duan L,Cheng S,Zhu X,Xi Y,Chang X,Ye Q,Gao S

Tue Apr 04 00:00:00 EDT 2023

The miR-27a-3p/FTO axis modifies hypoxia-induced malignant behaviors of glioma cells.

Acta biochimica et biophysica Sinica

Du P,Meng L,Liao X,Liu Y,Mo X,Gong M,Liao Y

Wed Jan 25 00:00:00 EST 2023

ALKBH1-mediated m¹ A demethylation of METTL3 mRNA promotes the metastasis of colorectal cancer by downregulating SMAD7 expression.

Molecular oncology

Chen W,Wang H,Mi S,Shao L,Xu Z,Xue M

Wed Feb 01 00:00:00 EST 2023

Differential epitranscriptome and proteome modulation in the brain of neonatal mice exposed to isoflurane or sevoflurane.

Cell biology and toxicology

Wu Y,Yang Z,Su S,Xu X,Li Y,Li X,Gao Y,Sun D,Wan S,Pen M,Jin W,Ke C

Sun Oct 01 00:00:00 EDT 2023

ALKBH5-PYCR2 Positive Feedback Loop Promotes Proneural-Mesenchymal Transition Via Proline Synthesis In GBM.

Journal of Cancer

Li L,Yang M,Pu X,Tang Y,Fei F,Li Z,Hou H,Chen Q,Wang Q,Wu Y,Zhang Y,Ren C,Gong A

Tue Oct 31 00:00:00 EDT 2023

Newcastle disease virus activates methylation-related enzymes to reprogram m⁶A methylation in infected cells.

Veterinary microbiology

Yuan W,Hou Y,Wang Q,Lv T,Ren J,Fan L,Cai J,Xiang B,Lin Q,Liao M,Ding C,Chen L,Ren T

Thu Jun 01 00:00:00 EDT 2023

Profile of N6-methyladenosine of Pb-exposed neurons presents epitranscriptomic alterations in PI3K-AKT pathway-associated genes.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Ai S,Li D,Gu X,Xu Y,Wang Y,Wang HL,Chen XT

Tue Aug 01 00:00:00 EDT 2023

IL-17A promotes endothelial cell senescence by up-regulating the expression of FTO through activating JNK signal pathway.

Biogerontology

Li N,Luo R,Zhang W,Wu Y,Hu C,Liu M,Jiang D,Jiang Z,Zhao X,Wang Y,Li Q

Wed Feb 01 00:00:00 EST 2023

N⁶-methyladenosine of Spi2a attenuates inflammation and sepsis-associated myocardial dysfunction in mice.

Nature communications

Wang X,Ding Y,Li R,Zhang R,Ge X,Gao R,Wang M,Huang Y,Zhang F,Zhao B,Liao W,Du J

Thu Mar 02 00:00:00 EST 2023

AS3MT facilitates NLRP3 inflammasome activation by m⁶A modification during arsenic-induced hepatic insulin resistance.

Cell biology and toxicology

Qiu T,Wu C,Yao X,Han Q,Wang N,Yuan W,Zhang J,Shi Y,Jiang L,Liu X,Yang G,Sun X

Sun Oct 01 00:00:00 EDT 2023

N⁶ -methyladenosine RNA demethylase FTO regulates extracellular matrix-related genes and promotes pancreatic cancer cell migration and invasion.

Cancer medicine

Wang W,He Y,Wu L,Zhai LL,Chen LJ,Yao LC,Yu KH,Tang ZG

Wed Feb 01 00:00:00 EST 2023

The differences in biological behavior and gene expression characteristics between pure and mixed early gastric signet ring cell carcinomas.

Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver

Wang X,Li X,Yang P,Qin X,Yu J,Cheng Y,Zhang G,Tang L

Thu Jun 01 00:00:00 EDT 2023

ZBTB7C m6A modification incurred by METTL3 aberration promotes osteosarcoma progression.

Translational research : the journal of laboratory and clinical medicine

An X,Wu W,Yang L,Dong J,Liu B,Guo J,Chen J,Guo B,Cao W,Jiang Q

Fri Sep 01 00:00:00 EDT 2023

ALKBH5 prevents hepatocellular carcinoma progression by post-transcriptional inhibition of PAQR4 in an m6A dependent manner.

Experimental hematology & oncology

Wang W,Huang Q,Liao Z,Zhang H,Liu Y,Liu F,Chen X,Zhang B,Chen Y,Zhu P

Fri Jan 06 00:00:00 EST 2023

FTO-dependent m⁶A modification of Plpp3 in circSCMH1-regulated vascular repair and functional recovery following stroke.

Nature communications

Li B,Xi W,Bai Y,Liu X,Zhang Y,Li L,Bian L,Liu C,Tang Y,Shen L,Yang L,Gu X,Xie J,Zhou Z,Wang Y,Yu X,Wang J,Chao J,Han B,Yao H

Mon Jan 30 00:00:00 EST 2023

Protective role of mRNA demethylase FTO on axon guidance molecules of nigro-striatal projection system in manganese-induced parkinsonism.

Journal of hazardous materials

Qi Z,Wang S,Li J,Wen Y,Cui R,Zhang K,Liu Y,Yang X,Zhang L,Xu B,Liu W,Xu Z,Deng Y

Tue Mar 15 00:00:00 EDT 2022

Reduced N6-Methyladenosine Mediated by METTL3 Acetylation Promotes MTF1 Expression and Hepatocellular Carcinoma Cell Growth.

Chemistry & biodiversity

Yang Y,Qian Cai Q,Sheng Fu L,Wei Dong Y,Fan F,Zhong Wu X

Tue Nov 01 00:00:00 EDT 2022

Novel insight of N⁶-methyladenosine modified subtypes in abdominal aortic aneurysm.

Frontiers in genetics

Wang K,Kan Q,Ye Y,Qiu J,Huang L,Wu R,Yao C

Sat Dec 10 00:00:00 EST 2022

Novel insight of N⁶-methyladenosine modified subtypes in abdominal aortic aneurysm.

Frontiers in genetics

Wang K,Kan Q,Ye Y,Qiu J,Huang L,Wu R,Yao C

Sat Dec 10 00:00:00 EST 2022

Identification and Characterization of a Glucometabolic Prognostic Gene Signature in Neuroblastoma based on N6-methyladenosine Eraser ALKBH5.

Journal of Cancer

Tan K,Wu W,Zhu K,Lu L,Lv Z

Sat Jul 16 00:00:00 EDT 2022

Identification and Characterization of a Glucometabolic Prognostic Gene Signature in Neuroblastoma based on N6-methyladenosine Eraser ALKBH5.

Journal of Cancer

Tan K,Wu W,Zhu K,Lu L,Lv Z

Sat Jul 16 00:00:00 EDT 2022

M⁶A RNA Methylation Mediates NOD1/NF-kB Signaling Activation in the Liver of Piglets Challenged with Lipopolysaccharide.

Antioxidants (Basel, Switzerland)

Xu M,Zhuo R,Tao S,Liang Y,Liu C,Liu Q,Wang T,Zhong X

Fri Sep 30 00:00:00 EDT 2022

m⁶A modification confers thermal vulnerability to HPV E7 oncotranscripts via reverse regulation of its reader protein IGF2BP1 upon heat stress.

Cell reports

Wang L,Zhan G,Maimaitiyiming Y,Su Y,Lin S,Liu J,Su K,Lin J,Shen S,He W,Wang F,Chen J,Sun S,Xue Y,Gu J,Chen X,Zhang J,Zhang L,Wang Q,Chang KJ,Chiou SH,Björklund M,Naranmandura H,Cheng X,Hsu CH

Tue Oct 25 00:00:00 EDT 2022

Comprehensive analysis of RNA m6A methylation in pressure overload-induced cardiac hypertrophy.

BMC genomics

Li W,Xing C,Bao L,Han S,Luo T,Wang Z,Fan H

Thu Aug 11 00:00:00 EDT 2022

RNA m⁶A demethylase ALKBH5 regulates the development of γδ T cells.

Proceedings of the National Academy of Sciences of the United States of America

Ding C,Xu H,Yu Z,Roulis M,Qu R,Zhou J,Oh J,Crawford J,Gao Y,Jackson R,Sefik E,Li S,Wei Z,Skadow M,Yin Z,Ouyang X,Wang L,Zou Q,Su B,Hu W,Flavell RA,Li HB

Tue Aug 16 00:00:00 EDT 2022

MiR-155 regulates m⁶A level and cell progression by targeting FTO in clear cell renal cell carcinoma.

Cellular signalling

Yang W,Xie L,Wang P,Zhuang C

Tue Mar 01 00:00:00 EST 2022

The Maternal Microbiome Programs the m⁶A Epitranscriptome of the Mouse Fetal Brain and Intestine.

Frontiers in cell and developmental biology

Xiao Z,Liu S,Li Z,Cui J,Wang H,Wang Z,Ren Q,Xia L,Wang Z,Li Y

Tue Jul 26 00:00:00 EDT 2022

The m6A-induced lncRNA CASC8 promotes proliferation and chemoresistance via upregulation of hnRNPL in esophageal squamous cell carcinoma.

International journal of biological sciences

Wu Q,Zhang H,Yang D,Min Q,Wang Y,Zhang W,Zhan Q

Mon Aug 22 00:00:00 EDT 2022

CircRNA CDR1as promotes cardiomyocyte apoptosis through activating hippo signaling pathway in diabetic cardiomyopathy.

European journal of pharmacology

Shao Y,Li M,Yu Q,Gong M,Wang Y,Yang X,Liu L,Liu D,Tan Z,Zhang Y,Qu Y,Li H,Wang Y,Jiao L,Zhang Y

Thu May 05 00:00:00 EDT 2022

The expression of m6A enzymes in the hippocampus of diabetic cognitive impairment mice and the possible improvement of YTHDF1.

Brain research

Li M,Zhong X,Zhao Z,Zeng Z,Yuan Q,Xiao X,Zu X,Liu J

Tue Feb 15 00:00:00 EST 2022

Downregulated ALKBH5 contributes to myocardial ischemia/reperfusion injury by increasing m⁶A modification of Trio mRNA.

Annals of translational medicine

Li J,Chen J,Zhao M,Li Z,Liu N,Fang H,Fang M,Zhu P,Lei L,Chen C

Fri Apr 01 00:00:00 EDT 2022

m6A RNA methylation regulates the transcription factors JUN and JUNB in TGF-β-induced epithelial-mesenchymal transition of lung cancer cells.

The Journal of biological chemistry

Suphakhong K,Terashima M,Wanna-Udom S,Takatsuka R,Ishimura A,Takino T,Suzuki T

Tue Nov 01 00:00:00 EDT 2022

PDGF signaling inhibits mitophagy in glioblastoma stem cells through N⁶-methyladenosine.

Developmental cell

Lv D,Gimple RC,Zhong C,Wu Q,Yang K,Prager BC,Godugu B,Qiu Z,Zhao L,Zhang G,Dixit D,Lee D,Shen JZ,Li X,Xie Q,Wang X,Agnihotri S,Rich JN

Mon Jun 20 00:00:00 EDT 2022

METTL14 Regulates Intestine Cellular Senescence through m⁶A Modification of Lamin B Receptor.

Oxidative medicine and cellular longevity

Zhang Z,Xue M,Chen J,Wang Z,Ju F,Ni J,Sun J,Wu H,Zheng H,Lou Z,Zhang Y,Yang X,Chen S,Xi Y,Wang L

Fri Dec 30 00:00:00 EST 2022

Aberrant DNA and RNA Methylation Occur in Spinal Cord and Skeletal Muscle of Human SOD1 Mouse Models of ALS and in Human ALS: Targeting DNA Methylation Is Therapeutic.

Cells

Martin LJ,Adams DA,Niedzwiecki MV,Wong M

Mon Oct 31 00:00:00 EDT 2022

ALKBH5/MAP3K8 axis regulates PD-L1+ macrophage infiltration and promotes hepatocellular carcinoma progression.

International journal of biological sciences

You Y,Wen D,Zeng L,Lu J,Xiao X,Chen Y,Song H,Liu Z

Mon Aug 22 00:00:00 EDT 2022

3D-Printed β-Tricalcium Phosphate Scaffolds Promote Osteogenic Differentiation of Bone Marrow-Deprived Mesenchymal Stem Cells in an N6-methyladenosine-Dependent Manner.

International journal of bioprinting

Jiao X,Sun X,Li W,Chu W,Zhang Y,Li Y,Wang Z,Zhou X,Ma J,Xu C,Dai K,Wang J,Gan Y

Sat Jul 16 00:00:00 EDT 2022

3D-Printed β-Tricalcium Phosphate Scaffolds Promote Osteogenic Differentiation of Bone Marrow-Deprived Mesenchymal Stem Cells in an N6-methyladenosine-Dependent Manner.

International journal of bioprinting

Jiao X,Sun X,Li W,Chu W,Zhang Y,Li Y,Wang Z,Zhou X,Ma J,Xu C,Dai K,Wang J,Gan Y

Sat Jul 16 00:00:00 EDT 2022

Systematic analysis of the expression profile and prognostic significance of m6A regulators and PD-L1 in hepatocellular carcinoma.

Discover. Oncology

Kong F,Wang K,Wang L

Fri Nov 25 00:00:00 EST 2022

RNA m⁶A Alterations Induced by Biomineralization Nanoparticles: A Proof-of-Concept Study of Epitranscriptomics for Nanotoxicity Evaluation.

Nanoscale research letters

Pan J,Wang J,Fang K,Hou W,Li B,Zhao J,Ma X

Sat Feb 05 00:00:00 EST 2022

N6-methyladenosine reader YTHDF3 regulates melanoma metastasis via its 'executor'LOXL3.

Clinical and translational medicine

Shi HZ,Xiong JS,Gan L,Zhang Y,Zhang CC,Kong YQ,Miao QJ,Tian CC,Li R,Liu JQ,Zhang EJ,Bu WB,Wang Y,Cheng XF,Sun JF,Chen H

Tue Nov 01 00:00:00 EDT 2022

m6A demethylation of cytidine deaminase APOBEC3B mRNA orchestrates arsenic-induced mutagenesis.

The Journal of biological chemistry

Gao M,Qi Z,Feng W,Huang H,Xu Z,Dong Z,Xu M,Han J,Kloeber JA,Huang J,Lou Z,Liu S

Tue Feb 01 00:00:00 EST 2022

Role of the Demethylase AlkB Homolog H5 in the Promotion of Dentinogenesis.

Frontiers in physiology

Tian C,Chai J,Liu W,Zhang X,Li Y,Zuo H,Yuan G,Zhang H,Liu H,Chen Z

Sat Jul 16 00:00:00 EDT 2022

Role of the Demethylase AlkB Homolog H5 in the Promotion of Dentinogenesis.

Frontiers in physiology

Tian C,Chai J,Liu W,Zhang X,Li Y,Zuo H,Yuan G,Zhang H,Liu H,Chen Z

Sat Jul 16 00:00:00 EDT 2022

Therapeutic targeting m6A-guided miR-146a-5p signaling contributes to the melittin-induced selective suppression of bladder cancer.

Cancer letters

Yan R,Dai W,Wu R,Huang H,Shu M

Sat May 28 00:00:00 EDT 2022

N6-methyladenosine RNA demethylase ALKBH5 is testis-specifically downregulated in hybrid male sterile dzo and is a target gene of bta-miR-200a.

Theriogenology

Chen H,Zhang J,Yan Y,Zhu C,Wang L,Fu S,Zuo F,Zhang GW

Fri Jul 15 00:00:00 EDT 2022

Mettl3 promotes oxLDL-mediated inflammation through activating STAT1 signaling.

Journal of clinical laboratory analysis

Li Z,Xu Q,Huangfu N,Chen X,Zhu J

Sat Jan 01 00:00:00 EST 2022

Decreased RNA m⁶A methylation enhances the process of the epithelial mesenchymal transition and vasculogenic mimicry in glioblastoma.

American journal of cancer research

Tao M,Li X,He L,Rong X,Wang H,Pan J,Lu Z,Zhang X,Peng Y

Thu Mar 10 00:00:00 EST 2022

Decreased RNA m⁶A methylation enhances the process of the epithelial mesenchymal transition and vasculogenic mimicry in glioblastoma.

American journal of cancer research

Tao M,Li X,He L,Rong X,Wang H,Pan J,Lu Z,Zhang X,Peng Y

Thu Mar 10 00:00:00 EST 2022

PGE₂ -EP3 axis promotes brown adipose tissue formation through stabilization of WTAP RNA methyltransferase.

The EMBO journal

Tao X,Du R,Guo S,Feng X,Yu T,OuYang Q,Chen Q,Fan X,Wang X,Guo C,Li X,Xue F,Chen S,Tong M,Lazarus M,Zuo S,Yu Y,Shen Y

Tue Aug 16 00:00:00 EDT 2022

The proteasome-dependent degradation of ALKBH5 regulates ECM deposition in PM_2.5 exposure-induced pulmonary fibrosis of mice.

Journal of hazardous materials

Zhang Y,Liu Q,Ning J,Jiang T,Kang A,Li L,Pang Y,Zhang B,Huang X,Wang Q,Bao L,Niu Y,Zhang R

Wed Jun 15 00:00:00 EDT 2022

The proteasome-dependent degradation of ALKBH5 regulates ECM deposition in PM_2.5 exposure-induced pulmonary fibrosis of mice.

Journal of hazardous materials

Zhang Y,Liu Q,Ning J,Jiang T,Kang A,Li L,Pang Y,Zhang B,Huang X,Wang Q,Bao L,Niu Y,Zhang R

Wed Jun 15 00:00:00 EDT 2022

The proteasome-dependent degradation of ALKBH5 regulates ECM deposition in PM_2.5 exposure-induced pulmonary fibrosis of mice.

Journal of hazardous materials

Zhang Y,Liu Q,Ning J,Jiang T,Kang A,Li L,Pang Y,Zhang B,Huang X,Wang Q,Bao L,Niu Y,Zhang R

Wed Jun 15 00:00:00 EDT 2022

Fusobacterium nucleatum reduces METTL3-mediated m⁶A modification and contributes to colorectal cancer metastasis.

Nature communications

Chen S,Zhang L,Li M,Zhang Y,Sun M,Wang L,Lin J,Cui Y,Chen Q,Jin C,Li X,Wang B,Chen H,Zhou T,Wang L,Hsu CH,Zhuo W

Thu Mar 10 00:00:00 EST 2022

Expression pattern of alkB homolog 5 in goat testis and its role in spermatogonial stem cells.

Cell and tissue research

Liu Z,Cai Y,Deng M,Li D,Leng Q,Shi L,Tang Y,Wang F,Wan Y

Sat Jan 01 00:00:00 EST 2022

Demethylase ALKBH5 suppresses invasion of gastric cancer via PKMYT1 m6A modification.

Molecular cancer

Hu Y,Gong C,Li Z,Liu J,Chen Y,Huang Y,Luo Q,Wang S,Hou Y,Yang S,Xiao Y

Thu Feb 03 00:00:00 EST 2022

Metformin combats obesity by targeting FTO in an m⁶A-YTHDF2-dependent manner.

Journal of drug targeting

Liao X,Liu J,Chen Y,Liu Y,Chen W,Zeng B,Liu Y,Luo Y,Huang C,Guo G,Wang Y,Wang X

Tue Nov 01 00:00:00 EDT 2022

ALKBH5-mediated m6A modification of circCCDC134 facilitates cervical cancer metastasis by enhancing HIF1A transcription.

Journal of experimental & clinical cancer research : CR

Liang L,Zhu Y,Li J,Zeng J,Wu L

Fri Aug 26 00:00:00 EDT 2022

ALKBH5-mediated m6A modification of circCCDC134 facilitates cervical cancer metastasis by enhancing HIF1A transcription.

Journal of experimental & clinical cancer research : CR

Liang L,Zhu Y,Li J,Zeng J,Wu L

Fri Aug 26 00:00:00 EDT 2022

ALKBH5-mediated m⁶A demethylation of KCNK15-AS1 inhibits pancreatic cancer progression via regulating KCNK15 and PTEN/AKT signaling.

Cell death & disease

He Y,Yue H,Cheng Y,Ding Z,Xu Z,Lv C,Wang Z,Wang J,Yin C,Hao H,Chen C

Wed Dec 01 00:00:00 EST 2021

Suppression of m6A mRNA modification by DNA hypermethylated ALKBH5 aggravates the oncological behavior of KRAS mutation/LKB1 loss lung cancer.

Cell death & disease

Zhang D,Ning J,Okon I,Zheng X,Satyanarayana G,Song P,Xu S,Zou MH

Thu May 20 00:00:00 EDT 2021

Suppression of m6A mRNA modification by DNA hypermethylated ALKBH5 aggravates the oncological behavior of KRAS mutation/LKB1 loss lung cancer.

Cell death & disease

Zhang D,Ning J,Okon I,Zheng X,Satyanarayana G,Song P,Xu S,Zou MH

Thu May 20 00:00:00 EDT 2021

m⁶A mRNA methylation regulates testosterone synthesis through modulating autophagy in Leydig cells.

Autophagy

Chen Y,Wang J,Xu D,Xiang Z,Ding J,Yang X,Li D,Han X

Mon Feb 01 00:00:00 EST 2021

m⁶A mRNA methylation regulates testosterone synthesis through modulating autophagy in Leydig cells.

Autophagy

Chen Y,Wang J,Xu D,Xiang Z,Ding J,Yang X,Li D,Han X

Mon Feb 01 00:00:00 EST 2021

YTHDF2 mediates LPS-induced osteoclastogenesis and inflammatory response via the NF-κB and MAPK signaling pathways.

Cellular signalling

Fang C,He M,Li D,Xu Q

Wed Sep 01 00:00:00 EDT 2021

Genome-wide map of N⁶-methyladenosine circular RNAs identified in mice model of severe acute pancreatitis.

World journal of gastroenterology

Wu J,Yuan XH,Jiang W,Lu YC,Huang QL,Yang Y,Qie HJ,Liu JT,Sun HY,Tang LJ

Sun Nov 21 00:00:00 EST 2021

The m⁶A methylome of SARS-CoV-2 in host cells.

Cell research

Liu J,Xu YP,Li K,Ye Q,Zhou HY,Sun H,Li X,Yu L,Deng YQ,Li RT,Cheng ML,He B,Zhou J,Li XF,Wu A,Yi C,Qin CF

Thu Apr 01 00:00:00 EDT 2021

Relaxed initiation pausing of ribosomes drives oncogenic translation.

Science advances

Dong L,Mao Y,Zhou A,Liu XM,Zhou J,Wan J,Qian SB

Mon Feb 01 00:00:00 EST 2021

AMD1 upregulates hepatocellular carcinoma cells stemness by FTO mediated mRNA demethylation.

Clinical and translational medicine

Bian X,Shi D,Xing K,Zhou H,Lu L,Yu D,Wu W

Mon Mar 01 00:00:00 EST 2021

Epitranscriptomic addition of m⁶A regulates HIV-1 RNA stability and alternative splicing.

Genes & development

Tsai K,Bogerd HP,Kennedy EM,Emery A,Swanstrom R,Cullen BR

Thu Jul 01 00:00:00 EDT 2021

Curcumin prevents obesity by targeting TRAF4-induced ubiquitylation in m⁶ A-dependent manner.

EMBO reports

Chen Y,Wu R,Chen W,Liu Y,Liao X,Zeng B,Guo G,Lou F,Xiang Y,Wang Y,Wang X

Wed May 05 00:00:00 EDT 2021

METTL3 regulates hippocampal gene transcription via N6-methyladenosine methylation in sevoflurane-induced postoperative cognitive dysfunction mouse.

Aging

He B,Wang J

Tue Oct 05 00:00:00 EDT 2021

The role of RNA m⁶A methylation in the regulation of postnatal hypoxia-induced pulmonary hypertension.

Respiratory research

Xu S,Xu X,Zhang Z,Yan L,Zhang L,Du L

Mon Apr 26 00:00:00 EDT 2021

Transcriptome-wide m⁶A methylome during osteogenic differentiation of human adipose-derived stem cells.

Stem cell research & therapy

Sun W,Song Y,Xia K,Yu L,Huang X,Zhao Z,Liu J

Wed Sep 01 00:00:00 EDT 2021

The Phytochemical Rhein Mediates M⁶A-Independent Suppression of Adipocyte Differentiation.

Frontiers in nutrition

Huang L,Zhang J,Zhu X,Mi X,Li Q,Gao J,Zhou J,Zhou J,Liu XM

Fri Nov 19 00:00:00 EST 2021

mTORC1 stimulates cell growth through SAM synthesis and m⁶A mRNA-dependent control of protein synthesis.

Molecular cell

Villa E,Sahu U,O'Hara BP,Ali ES,Helmin KA,Asara JM,Gao P,Singer BD,Ben-Sahra I

Thu May 20 00:00:00 EDT 2021

Remodeling of the m⁶A landscape in the heart reveals few conserved post-transcriptional events underlying cardiomyocyte hypertrophy.

Journal of molecular and cellular cardiology

Hinger SA,Wei J,Dorn LE,Whitson BA,Janssen PML,He C,Accornero F

Mon Feb 01 00:00:00 EST 2021

Upregulation of METTL3 expression and m6A RNA methylation in placental trophoblasts in preeclampsia.

Placenta

Gu Y,Chu X,Morgan JA,Lewis DF,Wang Y

Fri Jan 01 00:00:00 EST 2021

Upregulation of METTL3 expression and m6A RNA methylation in placental trophoblasts in preeclampsia.

Placenta

Gu Y,Chu X,Morgan JA,Lewis DF,Wang Y

Fri Jan 01 00:00:00 EST 2021

Human m⁶A-mRNA and lncRNA epitranscriptomic microarray reveal function of RNA methylation in hemoglobin H-constant spring disease.

Scientific reports

Ruan H,Yang F,Deng L,Yang D,Zhang X,Li X,Pang L

Thu Oct 14 00:00:00 EDT 2021

Streptozotocin-Induced Astrocyte Mitochondrial Dysfunction Is Ameliorated by FTO Inhibitor MO-I-500.

ACS chemical neuroscience

Cockova Z,Honc O,Telensky P,Olsen MJ,Novotny J

Wed Oct 20 00:00:00 EDT 2021

Adenylate Kinase 4 Modulates the Resistance of Breast Cancer Cells to Tamoxifen through an m⁶A-Based Epitranscriptomic Mechanism.

Molecular therapy : the journal of the American Society of Gene Therapy

Liu X,Gonzalez G,Dai X,Miao W,Yuan J,Huang M,Bade D,Li L,Sun Y,Wang Y

Wed Dec 02 00:00:00 EST 2020

Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma.

Medical science monitor : international medical journal of experimental and clinical research

Zhang Y,Liu X,Liu L,Li J,Hu Q,Sun R

Sat Feb 22 00:00:00 EST 2020

Resveratrol Attenuates Aflatoxin B₁-Induced ROS Formation and Increase of m⁶A RNA Methylation.

Animals : an open access journal from MDPI

Wu J,Gan Z,Zhuo R,Zhang L,Wang T,Zhong X

Mon Apr 13 00:00:00 EDT 2020

Resveratrol Attenuates High-Fat Diet Induced Hepatic Lipid Homeostasis Disorder and Decreases m⁶A RNA Methylation.

Frontiers in pharmacology

Wu J,Li Y,Yu J,Gan Z,Wei W,Wang C,Zhang L,Wang T,Zhong X

Tue Feb 02 00:00:00 EST 2021

METTL3 Modulates Osteoclast Differentiation and Function by Controlling RNA Stability and Nuclear Export.

International journal of molecular sciences

Li D,Cai L,Meng R,Feng Z,Xu Q

Fri Feb 28 00:00:00 EST 2020

N⁶-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2.

Nature genetics

Li Y,Xia L,Tan K,Ye X,Zuo Z,Li M,Xiao R,Wang Z,Liu X,Deng M,Cui J,Yang M,Luo Q,Liu S,Cao X,Zhu H,Liu T,Hu J,Shi J,Xiao S,Xia L

Tue Sep 01 00:00:00 EDT 2020

Alteration of N⁶-methyladenosine epitranscriptome profile in unilateral ureteral obstructive nephropathy.

Epigenomics

Li X,Fan X,Yin X,Liu H,Yang Y

Wed Jul 01 00:00:00 EDT 2020

The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N⁶-methyladenosine methylation of Parp10 mRNA.

Nature cell biology

Gao XQ,Zhang YH,Liu F,Ponnusamy M,Zhao XM,Zhou LY,Zhai M,Liu CY,Li XM,Wang M,Shan C,Shan PP,Wang Y,Dong YH,Qian LL,Yu T,Ju J,Wang T,Wang K,Chen XZ,Wang YH,Zhang J,Li PF,Wang K

Sun Nov 01 00:00:00 EDT 2020

The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N⁶-methyladenosine methylation of Parp10 mRNA.

Nature cell biology

Gao XQ,Zhang YH,Liu F,Ponnusamy M,Zhao XM,Zhou LY,Zhai M,Liu CY,Li XM,Wang M,Shan C,Shan PP,Wang Y,Dong YH,Qian LL,Yu T,Ju J,Wang T,Wang K,Chen XZ,Wang YH,Zhang J,Li PF,Wang K

Sun Nov 01 00:00:00 EDT 2020

AMPK facilitates intestinal long-chain fatty acid uptake by manipulating CD36 expression and translocation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Wu W,Wang S,Liu Q,Shan T,Wang X,Feng J,Wang Y

Wed Apr 01 00:00:00 EDT 2020

The m6A methyltransferase METTL3 cooperates with demethylase ALKBH5 to regulate osteogenic differentiation through NF-κB signaling.

Molecular and cellular biochemistry

Yu J,Shen L,Liu Y,Ming H,Zhu X,Chu M,Lin J

Wed Jan 01 00:00:00 EST 2020

N⁶-Methyladenosine Demethylase FTO Contributes to Neuropathic Pain by Stabilizing G9a Expression in Primary Sensory Neurons.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Li Y,Guo X,Sun L,Xiao J,Su S,Du S,Li Z,Wu S,Liu W,Mo K,Xia S,Chang YJ,Denis D,Tao YX

Wed Jul 01 00:00:00 EDT 2020

m⁶A in mRNA coding regions promotes translation via the RNA helicase-containing YTHDC2.

Nature communications

Mao Y,Dong L,Liu XM,Guo J,Ma H,Shen B,Qian SB

Mon Nov 25 00:00:00 EST 2019

HSP90 inhibitors stimulate DNAJB4 protein expression through a mechanism involving N⁶-methyladenosine.

Nature communications

Miao W,Li L,Zhao Y,Dai X,Chen X,Wang Y

Fri Aug 09 00:00:00 EDT 2019

Elevated N-methyltransferase expression induced by hepatic stellate cells contributes to the metastasis of hepatocellular carcinoma via regulation of the CD44v3 isoform.

Molecular oncology

Li J,You S,Zhang S,Hu Q,Wang F,Chi X,Zhao W,Xie C,Zhang C,Yu Y,Liu J,Zhao Y,Liu P,Zhang Y,Wei X,Li Q,Wang X,Yin Z

Sun Sep 01 00:00:00 EDT 2019

EBV epitranscriptome reprogramming by METTL14 is critical for viral-associated tumorigenesis.

PLoS pathogens

Lang F,Singh RK,Pei Y,Zhang S,Sun K,Robertson ES

Sat Jun 01 00:00:00 EDT 2019

Mettl3 Regulates Osteogenic Differentiation and Alternative Splicing of Vegfa in Bone Marrow Mesenchymal Stem Cells.

International journal of molecular sciences

Tian C,Huang Y,Li Q,Feng Z,Xu Q

Mon Jan 28 00:00:00 EST 2019

METTL3 Regulates Osteoblast Differentiation and Inflammatory Response via Smad Signaling and MAPK Signaling.

International journal of molecular sciences

Zhang Y,Gu X,Li D,Cai L,Xu Q

Fri Dec 27 00:00:00 EST 2019

N⁶-Methyladenosine Guides mRNA Alternative Translation during Integrated Stress Response.

Molecular cell

Zhou J,Wan J,Shu XE,Mao Y,Liu XM,Yuan X,Zhang X,Hess ME,Brüning JC,Qian SB

Thu Feb 15 00:00:00 EST 2018

N⁶-Methyladenosine Guides mRNA Alternative Translation during Integrated Stress Response.

Molecular cell

Zhou J,Wan J,Shu XE,Mao Y,Liu XM,Yuan X,Zhang X,Hess ME,Brüning JC,Qian SB

Thu Feb 15 00:00:00 EST 2018

Bioinformatics

Protein Aliases: AlkB family member 5, RNA demethylase; alkB, alkylation repair homolog 5; Alkylated DNA repair protein alkB homolog 5; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5; oxoglutarate and iron-dependent oxygenase domain containing; probable alpha-ketoglutarate-dependent dioxygenase ABH5; RNA demethylase ALKBH5

View more View less

Gene Aliases: ABH5; ALKBH5; AW050020; AW215868; E130207K11; OFOXD; OFOXD1; RGD1309496

View more View less

UniProt ID: (Human) Q6P6C2, (Mouse) Q3TSG4, (Rat) D3ZKD3

View more View less

Entrez Gene ID: (Human) 54890, (Mouse) 268420, (Rat) 303193

View more View less

Function(s)

oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors oxidative RNA demethylase activity poly(A) RNA binding metal ion binding sulfonate dioxygenase activity oxidoreductase activity 2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity procollagen-proline dioxygenase activity hypophosphite dioxygenase activity DNA-N1-methyladenine dioxygenase activity dioxygenase activity C-19 gibberellin 2-beta-dioxygenase activity C-20 gibberellin 2-beta-dioxygenase activity RNA processing factor

Process(es)

response to hypoxia positive regulation of defense response to virus by host mRNA processing mRNA export from nucleus spermatogenesis cell differentiation oxidative single-stranded RNA demethylation xenophagy oxidation-reduction process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

ALKBH5 Polyclonal Antibody

ALKBH5 Antibody (16837-1-AP) in IHC (P)

Product Details

16837-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!