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Product Details
67811-1-IG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
2D2B2
Immunogen
ALKBH5 Fusion Protein Ag10490
Conjugate
Form
Liquid
Concentration
1000 µg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
Product Specific Information
Aliquoting is unnecessary for -20°C storage.
Target Information
ALKBH5 (RNA demehtylase ALKBH5) is a dioxygenase that demethylates RNA by oxidative demethylation. Specifically, it demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Demethylation of m6A mRNA affects mRNA processing and export. ALKBH5 can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). It requires molecular oxygen, alpha-ketoglutarate and iron.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AlkB family member 5, RNA demethylase; alkB, alkylation repair homolog 5; Alkylated DNA repair protein alkB homolog 5; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5; oxoglutarate and iron-dependent oxygenase domain containing; probable alpha-ketoglutarate-dependent dioxygenase ABH5; RNA demethylase ALKBH5
Gene Aliases: ABH5; ALKBH5; AW050020; AW215868; E130207K11; OFOXD; OFOXD1; RGD1309496
UniProt ID: (Human) Q6P6C2, (Mouse) Q3TSG4, (Rat) D3ZKD3
Entrez Gene ID: (Human) 54890, (Mouse) 268420, (Rat) 303193
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
oxidative RNA demethylase activity
poly(A) RNA binding
metal ion binding
sulfonate dioxygenase activity
oxidoreductase activity
2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity
procollagen-proline dioxygenase activity
hypophosphite dioxygenase activity
DNA-N1-methyladenine dioxygenase activity
dioxygenase activity
C-19 gibberellin 2-beta-dioxygenase activity
C-20 gibberellin 2-beta-dioxygenase activity
RNA processing factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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