Proteintech

ALPL Polyclonal Antibody

ALPL Antibody in Immunocytochemistry (ICC/IF)

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ALPL Antibody (11187-1-AP) in ICC/IF

Immunofluorescent analysis of HEK-293 cells using 11187-1-AP (ALPL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

ALPL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

11187-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 27 publications 27 publications

Immunohistochemistry (IHC)

View 16 publications 16 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:50

View 2 publications 2 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

ChIP assay (ChIP)

Assay-dependent

View 1 publication 1 publication

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ALPL Fusion Protein Ag1664 (23-330 aa encoded by BC021289)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody can bind the four mentioned alkaline phosphatases.

Immunogen sequence: EKDPKYWRD QAQETLKYAL ELQKLNTNVA KNVIMFLGDG MGVSTVTAAR ILKGQLHHNP GEETRLEMDK FPFVALSKTY NTNAQVPDSA GTATAYLCGV KANEGTVGVS AATERSRCNT TQGNEVTSIL RWAKDAGKSV GIVTTTRVNH ATPSAAYAHS ADRDWYSDNE MPPEALSQGC KDIAYQLMHN IRDIDVIMGG GRKYMYPKNK TDVEYESDEK ARGTRLDGLD LVDTWKSFKP RYKHSHFIWN RTELLTLDPH NVDYLLGLFE PGDMQYELNR NNVTDPSLSE MVVVAIQILR KNPKGFFLL (23-330 aa encoded by BC021289)

Target Information

Alkaline phosphatase is a homodimeric protein enzyme (86 kDa), containing two zinc atoms crucial to its catalytic function per monomer, and it is optimally active at alkaline pH environments. The enzyme is found in prokaryotes and eukaryotes, with the same general function but in different structural forms suitable to organism's environment. ALP plays a role in liver metabolism and development within the skeleton. In bacteria, ALP acts as a source of inorganic phosphate. In yeast, ALP can be a useful protein for studying membrane protein sorting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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An in vivo humanized model to study homing and sequestration of Plasmodium falciparum transmission stages in the bone marrow.

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Dihydromyricetin ameliorates osteogenic differentiation of human aortic valve interstitial cells by targeting c-KIT/interleukin-6 signaling pathway.

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Zhang S,Fan L,Wang Y,Xu J,Shen Q,Xie J,Zeng Z,Zhou T

Fri Aug 26 00:00:00 EDT 2022

Dihydromyricetin ameliorates osteogenic differentiation of human aortic valve interstitial cells by targeting c-KIT/interleukin-6 signaling pathway.

Frontiers in pharmacology

Zhang S,Fan L,Wang Y,Xu J,Shen Q,Xie J,Zeng Z,Zhou T

Fri Aug 26 00:00:00 EDT 2022

Lansoprazole-induced osteoporosis via the IP3R- and SOCE-mediated calcium signaling pathways.

Molecular medicine (Cambridge, Mass.)

Cheng Z,Liu Y,Ma M,Sun S,Ma Z,Wang Y,Yu L,Qian X,Sun L,Zhang X,Liu Y,Wang Y

Sat Feb 19 00:00:00 EST 2022

Cellular communication network factor 1 interlinks autophagy and ERK signaling to promote osteogenesis of periodontal ligament stem cells.

Journal of periodontal research

Li Z,Wu Z,Xi X,Zhao F,Liu H,Liu D

Thu Dec 01 00:00:00 EST 2022

Chronic Alcohol Reduces Bone Mass Through Inhibiting Proliferation and Promoting Aging of Endothelial Cells in Type-H Vessels.

Stem cells and development

Chen A,Li X,Zhao J,Zhou J,Xie C,Chen H,Wang Q,Wang R,Miao D,Li J,Jin J

Thu Sep 01 00:00:00 EDT 2022

Gsα^R201C and estrogen reveal different subsets of bone marrow adiponectin expressing osteogenic cells.

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Palmisano B,Labella R,Donsante S,Remoli C,Spica E,Coletta I,Farinacci G,Dello Spedale Venti M,Saggio I,Serafini M,Robey PG,Corsi A,Riminucci M

Tue Jul 19 00:00:00 EDT 2022

Mechanical force-sensitive lncRNA SNHG8 inhibits osteogenic differentiation by regulating EZH2 in hPDLSCs.

Cellular signalling

Zhang Z,He Q,Yang S,Zhao X,Li X,Wei F

Sun May 01 00:00:00 EDT 2022

N-acetylcysteine promotes cyclic mechanical stress-induced osteogenic differentiation of periodontal ligament stem cells by down-regulating Nrf2 expression.

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Xi X,Li ZX,Zhao Y,Liu H,Chen S,Liu DX

Fri Apr 01 00:00:00 EDT 2022

Circular RNA circSmoc1-2 regulates vascular calcification by acting as a miR-874-3p sponge in vascular smooth muscle cells.

Molecular therapy. Nucleic acids

Ryu J,Choe N,Kwon DH,Shin S,Lim YH,Yoon G,Kim JH,Kim HS,Lee IK,Ahn Y,Park WJ,Kook H,Kim YK

Tue Mar 08 00:00:00 EST 2022

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Virologica Sinica

Song Y,Shou S,Guo H,Gao Z,Liu N,Yang Y,Wang F,Deng Q,Liu J,Xie Y

Mon Aug 01 00:00:00 EDT 2022

Gold nanoparticles targeting the autophagy-lysosome system to combat the inflammation-compromised osteogenic potential of periodontal ligament stem cells: From mechanism to therapy.

Biomaterials

Yin Y,Tian BM,Li X,Yu YC,Deng DK,Sun LJ,Qu HL,Wu RX,Xu XY,Sun HH,An Y,He XT,Chen FM

Thu Sep 01 00:00:00 EDT 2022

Polydopamine-Coated Poly(l-lactide) Nanofibers with Controlled Release of VEGF and BMP-2 as a Regenerative Periosteum.

ACS biomaterials science & engineering

Sun H,Dong J,Wang Y,Shen S,Shi Y,Zhang L,Zhao J,Sun X,Jiang Q

Mon Oct 11 00:00:00 EDT 2021

Cell dynamics in Hertwig's epithelial root sheath are regulated by β-catenin activity during tooth root development.

Journal of cellular physiology

Yang S,Choi H,Kim TH,Jeong JK,Liu Y,Harada H,Cho ES

Thu Jul 01 00:00:00 EDT 2021

IL-18-Mediated SLC7A5 Overexpression Enhances Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells via the c-MYC Pathway.

Frontiers in cell and developmental biology

Ni F,Zhang T,Xiao W,Dong H,Gao J,Liu Y,Li J

Tue Jan 04 00:00:00 EST 2022

CDR1as regulated by hnRNPM maintains stemness of periodontal ligament stem cells via miR-7/KLF4.

Journal of cellular and molecular medicine

Gu X,Li X,Jin Y,Zhang Z,Li M,Liu D,Wei F

Sat May 01 00:00:00 EDT 2021

Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction.

Frontiers in cell and developmental biology

Xi X,Li Z,Liu H,Chen S,Liu D

Tue Jan 25 00:00:00 EST 2022

The effects of Omarigliptin on promoting osteoblastic differentiation.

Bioengineered

Liao F,Hu X,Chen R

Wed Dec 01 00:00:00 EST 2021

Metformin coordinates osteoblast/osteoclast differentiation associated with ischemic osteonecrosis.

Aging

Park SH,Kang MA,Moon YJ,Jang KY,Kim JR

Tue Feb 11 00:00:00 EST 2020

MnTBAP inhibits bone loss in ovariectomized rats by reducing mitochondrial oxidative stress in osteoblasts.

Journal of bone and mineral metabolism

Cao X,Luo D,Li T,Huang Z,Zou W,Wang L,Lian K,Lin D

Wed Jan 01 00:00:00 EST 2020

Musashi2 promotes EGF-induced EMT in pancreatic cancer via ZEB1-ERK/MAPK signaling.

Journal of experimental & clinical cancer research : CR

Sheng W,Shi X,Lin Y,Tang J,Jia C,Cao R,Sun J,Wang G,Zhou L,Dong M

Fri Jan 17 00:00:00 EST 2020

Experimental study of expression profile and specific role of human microRNAs in regulating atrophic bone nonunion.

Medicine

Wei J,Chen H,Fu Y,Zhang B,Zhang L,Tao S,Lin F

Fri Sep 04 00:00:00 EDT 2020

Inhibition of vascular calcification by microRNA-155-5p is accompanied by the inactivation of TGF-β1/Smad2/3 signaling pathway.

Acta histochemica

Zhao F,Wu Y,Yang W,Wu D,Wang C,Zhang F

Fri May 01 00:00:00 EDT 2020

VPS4B mutation impairs the osteogenic differentiation of dental follicle cells derived from a patient with dentin dysplasia type I.

International journal of oral science

Li Q,Lu F,Chen T,Zhang K,Lu Y,Li X,Wang Y,Liu L,Tian Q,Xiong F,Chen D

Fri Jul 31 00:00:00 EDT 2020

Neuropeptide Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3‑E1 cells via an autocrine mechanism.

Molecular medicine reports

Zhang B,Zhang X,Xiao J,Zhou X,Chen Y,Gao C

Sun Nov 01 00:00:00 EDT 2020

MicroRNA-21 promotes bone reconstruction in maxillary bone defects.

Journal of oral rehabilitation

Wang H,Wang H,Li X,Zhang Z,Zhao X,Wang C,Wei F

Sun Nov 01 00:00:00 EDT 2020

Effect of MicroRNA-20a on Osteogenic Differentiation of Human Adipose Tissue-Derived Stem Cells.

Cells, tissues, organs

Luo T,Yang X,Sun Y,Huang X,Zou L,Liu J

Tue Jul 07 00:00:00 EDT 2020

miR-34a promotes bone regeneration in irradiated bone defects by enhancing osteoblastic differentiation of mesenchymal stromal cells in rats.

Stem cell research & therapy

Liu H,Dong Y,Feng X,Li L,Jiao Y,Bai S,Feng Z,Yu H,Li X,Zhao Y

Tue Jun 18 00:00:00 EDT 2019

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Cell death & disease

Li J,Li X,Liu D,Hamamura K,Wan Q,Na S,Yokota H,Zhang P

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Transcriptome analysis of gingival tissues of enamel-renal syndrome.

Journal of periodontal research

Wang YP,Lin HY,Zhong WL,Simmer JP,Wang SK

Sun Dec 01 00:00:00 EST 2019

Silver nanoparticles promote osteogenic differentiation of human periodontal ligament fibroblasts by regulating the RhoA-TAZ axis.

Cell biology international

Xu Y,Zheng B,He J,Cui Z,Liu Y

Thu Aug 01 00:00:00 EDT 2019

Myricetin ameliorates glucocorticoid-induced osteoporosis through the ERK signaling pathway.

Life sciences

Fan S,Gao X,Chen P,Li X

Wed Aug 15 00:00:00 EDT 2018

Myricetin ameliorates glucocorticoid-induced osteoporosis through the ERK signaling pathway.

Life sciences

Fan S,Gao X,Chen P,Li X

Wed Aug 15 00:00:00 EDT 2018

Effect of propolis on preserving human periodontal ligament cells and regulating pro-inflammatory cytokines.

Dental traumatology : official publication of International Association for Dental Traumatology

Yuan XJ,Wang YY,Shi BQ,Zhao YM

Sun May 27 00:00:00 EDT 2018

Effect of propolis on preserving human periodontal ligament cells and regulating pro-inflammatory cytokines.

Dental traumatology : official publication of International Association for Dental Traumatology

Yuan XJ,Wang YY,Shi BQ,Zhao YM

Sun May 27 00:00:00 EDT 2018

Endothelial progenitor cells improve the therapeutic effect of mesenchymal stem cell sheets on irradiated bone defect repair in a rat model.

Journal of translational medicine

Liu H,Jiao Y,Zhou W,Bai S,Feng Z,Dong Y,Liu Q,Feng X,Zhao Y

Tue May 22 00:00:00 EDT 2018

Endothelial progenitor cells improve the therapeutic effect of mesenchymal stem cell sheets on irradiated bone defect repair in a rat model.

Journal of translational medicine

Liu H,Jiao Y,Zhou W,Bai S,Feng Z,Dong Y,Liu Q,Feng X,Zhao Y

Tue May 22 00:00:00 EDT 2018

Effects of nicotine on the metabolism and gene expression profile of Sprague‑Dawley rat primary osteoblasts.

Molecular medicine reports

Liang D,Wang KJ,Tang ZQ,Liu RH,Zeng F,Cheng MY,Lian QS,Wu HK

Fri Jun 01 00:00:00 EDT 2018

The microRNA-10a/ID3/RUNX2 axis modulates the development of Ossification of Posterior Longitudinal Ligament.

Scientific reports

Xu C,Zhang H,Gu W,Wu H,Chen Y,Zhou W,Sun B,Shen X,Zhang Z,Wang Y,Liu Y,Yuan W

Fri Jun 15 00:00:00 EDT 2018

The microRNA-10a/ID3/RUNX2 axis modulates the development of Ossification of Posterior Longitudinal Ligament.

Scientific reports

Xu C,Zhang H,Gu W,Wu H,Chen Y,Zhou W,Sun B,Shen X,Zhang Z,Wang Y,Liu Y,Yuan W

Fri Jun 15 00:00:00 EDT 2018

Improvement of bone defect healing in rats via mesenchymal stem cell supernatant.

Experimental and therapeutic medicine

Zhou W,Liu Q,Xu B

Thu Feb 01 00:00:00 EST 2018

Testicular acid phosphatase induces odontoblast differentiation and mineralization.

Cell and tissue research

Choi H,Kim TH,Yun CY,Kim JW,Cho ES

Fri Apr 01 00:00:00 EDT 2016

Testicular acid phosphatase induces odontoblast differentiation and mineralization.

Cell and tissue research

Choi H,Kim TH,Yun CY,Kim JW,Cho ES

Fri Apr 01 00:00:00 EDT 2016

Disruption of Tgfbr2 in odontoblasts leads to aberrant pulp calcification.

Journal of dental research

Ahn YH,Kim TH,Choi H,Bae CH,Yang YM,Baek JA,Lee JC,Cho ES

Mon Jun 01 00:00:00 EDT 2015

Disruption of Tgfbr2 in odontoblasts leads to aberrant pulp calcification.

Journal of dental research

Ahn YH,Kim TH,Choi H,Bae CH,Yang YM,Baek JA,Lee JC,Cho ES

Mon Jun 01 00:00:00 EDT 2015

Osterix regulates tooth root formation in a site-specific manner.

Journal of dental research

Kim TH,Bae CH,Lee JC,Kim JE,Yang X,de Crombrugghe B,Cho ES

Sun Mar 01 00:00:00 EST 2015

Osterix regulates tooth root formation in a site-specific manner.

Journal of dental research

Kim TH,Bae CH,Lee JC,Kim JE,Yang X,de Crombrugghe B,Cho ES

Sun Mar 01 00:00:00 EST 2015

Magnesium ion implantation on a micro/nanostructured titanium surface promotes its bioactivity and osteogenic differentiation function.

International journal of nanomedicine

Wang G,Li J,Zhang W,Xu L,Pan H,Wen J,Wu Q,She W,Jiao T,Liu X,Jiang X

Wed Dec 10 00:00:00 EST 2014

Magnesium ion implantation on a micro/nanostructured titanium surface promotes its bioactivity and osteogenic differentiation function.

International journal of nanomedicine

Wang G,Li J,Zhang W,Xu L,Pan H,Wen J,Wu Q,She W,Jiao T,Liu X,Jiang X

Wed Dec 10 00:00:00 EST 2014

Role of Wnt/β-catenin signaling pathway in the mechanism of calcification of aortic valve.

Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban

Gu GJ,Chen T,Zhou HM,Sun KX,Li J

Sat Feb 01 00:00:00 EST 2014

Role of Wnt/β-catenin signaling pathway in the mechanism of calcification of aortic valve.

Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban

Gu GJ,Chen T,Zhou HM,Sun KX,Li J

Sat Feb 01 00:00:00 EST 2014

β-catenin is required in odontoblasts for tooth root formation.

Journal of dental research

Kim TH,Bae CH,Lee JC,Ko SO,Yang X,Jiang R,Cho ES

Fri Mar 01 00:00:00 EST 2013

New population of odontoblasts responsible for tooth root formation.

Gene expression patterns : GEP

Bae CH,Kim TH,Chu JY,Cho ES

Tue Jan 07 00:00:00 EST 2014

New population of odontoblasts responsible for tooth root formation.

Gene expression patterns : GEP

Bae CH,Kim TH,Chu JY,Cho ES

Tue Jan 07 00:00:00 EST 2014

BMP2/7 heterodimer is a stronger inducer of bone regeneration in peri-implant bone defects model than BMP2 or BMP7 homodimer.

Dental materials journal

Sun P,Wang J,Zheng Y,Fan Y,Gu Z

Tue Sep 11 00:00:00 EDT 2012

BMP2/7 heterodimer is a stronger inducer of bone regeneration in peri-implant bone defects model than BMP2 or BMP7 homodimer.

Dental materials journal

Sun P,Wang J,Zheng Y,Fan Y,Gu Z

Tue Sep 11 00:00:00 EDT 2012

Col1a1-cre mediated activation of β-catenin leads to aberrant dento-alveolar complex formation.

Anatomy & cell biology

Kim TH,Bae CH,Jang EH,Yoon CY,Bae Y,Ko SO,Taketo MM,Cho ES

Sat Sep 01 00:00:00 EDT 2012

Col1a1-cre mediated activation of β-catenin leads to aberrant dento-alveolar complex formation.

Anatomy & cell biology

Kim TH,Bae CH,Jang EH,Yoon CY,Bae Y,Ko SO,Taketo MM,Cho ES

Sat Sep 01 00:00:00 EDT 2012

Constitutive stabilization of ß-catenin in the dental mesenchyme leads to excessive dentin and cementum formation.

Biochemical and biophysical research communications

Kim TH,Lee JY,Baek JA,Lee JC,Yang X,Taketo MM,Jiang R,Cho ES

Fri Sep 09 00:00:00 EDT 2011

Constitutive stabilization of ß-catenin in the dental mesenchyme leads to excessive dentin and cementum formation.

Biochemical and biophysical research communications

Kim TH,Lee JY,Baek JA,Lee JC,Yang X,Taketo MM,Jiang R,Cho ES

Fri Sep 09 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: Alkaline phosphatase liver/bone/kidney isozyme; Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; FLJ40094; FLJ93059; glycerophosphatase; liver/bone/kidney-type alkaline phosphatase; MGC161443; MGC167935; Phosphoamidase; Phosphocreatine phosphatase; tissue-nonspecific ALP

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Gene Aliases: ALPL; AP-TNAP; APTNAP; HOPS; TNAP; TNSALP

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UniProt ID: (Human) P05186

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Entrez Gene ID: (Human) 249

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Function(s)

alkaline phosphatase activity protein binding pyrophosphatase activity metal ion binding phosphatase

Process(es)

skeletal system development osteoblast differentiation endochondral ossification developmental process involved in reproduction dephosphorylation response to lipopolysaccharide response to vitamin D response to antibiotic response to glucocorticoid cellular response to organic cyclic compound cementum mineralization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ALPL Polyclonal Antibody

ALPL Antibody (11187-1-AP) in ICC/IF

Product Details

11187-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ChIP assay (ChIP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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