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Invitrogen
ALPL Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
ALPL Antibody (PA5-47419) in ICC/IF
Immunocytochemistry analysis of ALPL in immersion fixed rat mesenchymal stem cells. Samples were incubated in ALPL polyclonal antibody (Product # PA5-47419) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm.
Product Details
PA5-47419
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Alkaline Phosphatase/ALPL Phe18-Gly503
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609590
Product Specific Information
In direct ELISAs and Western blots, approximately 60% cross-reactivity with recombinant human ALPL is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Alkaline phosphatase is a homodimeric protein enzyme (86 kDa), containing two zinc atoms crucial to its catalytic function per monomer, and it is optimally active at alkaline pH environments. The enzyme is found in prokaryotes and eukaryotes, with the same general function but in different structural forms suitable to organism's environment. ALP plays a role in liver metabolism and development within the skeleton. In bacteria, ALP acts as a source of inorganic phosphate. In yeast, ALP can be a useful protein for studying membrane protein sorting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alkaline phosphatase 2; alkaline phosphatase 2, liver; Alkaline phosphatase liver/bone/kidney isozyme; Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; FLJ40094; FLJ93059; glycerophosphatase; liver/bone/kidney-type alkaline phosphatase; MGC161443; MGC167935; Phosphoamidase; Phosphocreatine phosphatase; tissue-nonspecific ALP
Gene Aliases: Akp-2; Akp2; ALPL; AP-TNAP; APTNAP; HOPS; TNAP; TNSALP
UniProt ID: (Human) P05186, (Mouse) P09242
Entrez Gene ID: (Human) 249, (Mouse) 11647
skeletal system development
osteoblast differentiation
endochondral ossification
developmental process involved in reproduction
dephosphorylation
response to lipopolysaccharide
response to vitamin D
response to antibiotic
response to glucocorticoid
cellular response to organic cyclic compound
cementum mineralization
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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