Proteintech

AMFR Polyclonal Antibody

AMFR Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

AMFR Antibody (16675-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using AMFR antibody 16675-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

AMFR Antibody in Immunohistochemistry (Paraffin) (IHC (P))

AMFR Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

16675-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 21 publications 21 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:10-1:100

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

AMFR Fusion Protein Ag10178 (301-643 aa encoded by BC069197)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.24 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LFHEVQRRI RRHKNYLRVV GNMEARFAVA TPEELAVNND DCAICWDSMQ AARKLPCGHL FHNSCLRSWL EQDTSCPTCR MSLNIADNNR VREEHQGENL DENLVPVAAA EGRPRLNQHN HFFHFDGSRI ASWLPSFSVE VMHTTNILGI TQASNSQLNA MAHQIQEMFP QVPYHLVLQD LQLTRSVEIT TDNILEGRIQ VPFPTQRSDS IRPALNSPVE RPSSDQEEGE TSAQTERVPL DLSPRLEETL DFGEVEVEPS EVEDFEARGS RFSKSADERQ RMLVQRKDEL LQQARKRFLN KSSEDDAASE SFLPSEGASS DPVTLRRRML AAAAERRLQK QQTS (301-643 aa encoded by B C069197)

Target Information

Autocrine motility factor is a tumor motility-stimulating protein secreted by tumor cells. The protein encoded by this gene is a glycosylated transmembrane protein and a receptor for autocrine motility factor. The receptor, which shows some sequence similarity to tumor protein p53, is localized to the leading and trailing edges of carcinoma cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

1,25(OH)₂ D₃ induced vitamin D receptor signaling negatively regulates endoplasmic reticulum-associated degradation (ERAD) and androgen receptor signaling in human prostate cancer cells.

Cellular signalling

Erzurumlu Y,Aydogdu E,Dogan HK,Catakli D,Muhammed MT,Buyuksandic B

Wed Mar 01 00:00:00 EST 2023

The chemokine CCL1 facilitates pulmonary fibrosis by promoting macrophage migration and M2 polarization.

International immunopharmacology

Liu S,Zhang Z,Wang Y,Zhang Y,Min J,Li X,Liu S

Sat Jul 01 00:00:00 EDT 2023

Estrogens drive the endoplasmic reticulum-associated degradation and promote proto-oncogene c-Myc expression in prostate cancer cells by androgen receptor/estrogen receptor signaling.

Journal of cell communication and signaling

Erzurumlu Y,Dogan HK,Catakli D,Aydogdu E,Muhammed MT

Fri Sep 01 00:00:00 EDT 2023

Ubiquitination regulates ER-phagy and remodelling of endoplasmic reticulum.

Nature

González A,Covarrubias-Pinto A,Bhaskara RM,Glogger M,Kuncha SK,Xavier A,Seemann E,Misra M,Hoffmann ME,Bräuning B,Balakrishnan A,Qualmann B,Dötsch V,Schulman BA,Kessels MM,Hübner CA,Heilemann M,Hummer G,Dikić I

Thu Jun 01 00:00:00 EDT 2023

Triiodothyronine positively regulates endoplasmic reticulum-associated degradation (ERAD) and promotes androgenic signaling in androgen-dependent prostate cancer cells.

Cellular signalling

Erzurumlu Y,Muhammed MT

Fri Sep 01 00:00:00 EDT 2023

Dexamethasone-stimulated glucocorticoid receptor signaling positively regulates the endoplasmic reticulum-associated degradation (ERAD) mechanism in hepatocellular carcinoma cells.

Steroids

Erzurumlu Y,Dogan HK,Cataklı D

Sat Jul 01 00:00:00 EDT 2023

Progesterone regulates the endoplasmic reticulum-associated degradation and Unfolded Protein Response axis by mimicking the androgenic stimulation in prostate cancer cells.

Molecular biology reports

Erzurumlu Y,Dogan HK,Catakli D

Wed Feb 01 00:00:00 EST 2023

Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy.

Nature

Foronda H,Fu Y,Covarrubias-Pinto A,Bocker HT,González A,Seemann E,Franzka P,Bock A,Bhaskara RM,Liebmann L,Hoffmann ME,Katona I,Koch N,Weis J,Kurth I,Gleeson JG,Reggiori F,Hummer G,Kessels MM,Qualmann B,Mari M,Dikić I,Hübner CA

Thu Jun 01 00:00:00 EDT 2023

The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition.

Nature communications

Ganji R,Paulo JA,Xi Y,Kline I,Zhu J,Clemen CS,Weihl CC,Purdy JG,Gygi SP,Raman M

Mon Feb 06 00:00:00 EST 2023

Regulation of membrane fluidity by RNF145-triggered degradation of the lipid hydrolase ADIPOR2.

The EMBO journal

Volkmar N,Gawden-Bone CM,Williamson JC,Nixon-Abell J,West JA,St George-Hyslop PH,Kaser A,Lehner PJ

Tue Oct 04 00:00:00 EDT 2022

Pharmacological induction of AMFR increases functional EAAT2 oligomer levels and reduces epileptic seizures in mice.

JCI insight

Sha L,Li G,Zhang X,Lin Y,Qiu Y,Deng Y,Zhu W,Xu Q

Mon Aug 08 00:00:00 EDT 2022

Pharmacological induction of AMFR increases functional EAAT2 oligomer levels and reduces epileptic seizures in mice.

JCI insight

Sha L,Li G,Zhang X,Lin Y,Qiu Y,Deng Y,Zhu W,Xu Q

Mon Aug 08 00:00:00 EDT 2022

Pharmacological induction of AMFR increases functional EAAT2 oligomer levels and reduces epileptic seizures in mice.

JCI insight

Sha L,Li G,Zhang X,Lin Y,Qiu Y,Deng Y,Zhu W,Xu Q

Mon Aug 08 00:00:00 EDT 2022

The GR-gp78 Pathway is involved in Hepatic Lipid Accumulation Induced by Overexpression of 11β-HSD1.

International journal of biological sciences

Hu M,Han T,Pan Q,Ni D,Gao F,Wang L,Ren H,Zhang X,Jiao H,Wang Y,Dai D,Man Y,Tang W,Sun Y,Li W,Li J,Li G

Thu Jun 02 00:00:00 EDT 2022

AAA-ATPase valosin-containing protein binds the transcription factor SREBP1 and promotes its proteolytic activation by rhomboid protease RHBDL4.

The Journal of biological chemistry

Shibuya K,Ebihara K,Ebihara C,Sawayama N,Isoda M,Yamamuro D,Takahashi M,Nagashima S,Ishibashi S

Wed Jun 01 00:00:00 EDT 2022

Basal Gp78-dependent mitophagy promotes mitochondrial health and limits mitochondrial ROS.

Cellular and molecular life sciences : CMLS

Alan P,Vandevoorde KR,Joshi B,Cardoen B,Gao G,Mohammadzadeh Y,Hamarneh G,Nabi IR

Tue Oct 25 00:00:00 EDT 2022

ERAD components Derlin-1 and Derlin-2 are essential for postnatal brain development and motor function.

iScience

Sugiyama T,Murao N,Kadowaki H,Takao K,Miyakawa T,Matsushita Y,Katagiri T,Futatsugi A,Shinmyo Y,Kawasaki H,Sakai J,Shiomi K,Nakazato M,Takeda K,Mikoshiba K,Ploegh HL,Ichijo H,Nishitoh H

Fri Jul 23 00:00:00 EDT 2021

Spermine Protects Cardiomyocytes from High Glucose-Induced Energy Disturbance by Targeting the CaSR-gp78-Ubiquitin Proteasome System.

Cardiovascular drugs and therapy

Wang Y,Wang Y,Li F,Zhang X,Li H,Yang G,Xu C,Wei C

Mon Feb 01 00:00:00 EST 2021

Reticulon and CLIMP-63 regulate nanodomain organization of peripheral ER tubules.

PLoS biology

Gao G,Zhu C,Liu E,Nabi IR

Thu Aug 01 00:00:00 EDT 2019

MARCH6 and TRC8 facilitate the quality control of cytosolic and tail-anchored proteins.

EMBO reports

Stefanovic-Barrett S,Dickson AS,Burr SP,Williamson JC,Lobb IT,van den Boomen DJ,Lehner PJ,Nathan JA

Tue May 01 00:00:00 EDT 2018

The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1.

eLife

Menzies SA,Volkmar N,van den Boomen DJ,Timms RT,Dickson AS,Nathan JA,Lehner PJ

Thu Dec 13 00:00:00 EST 2018

The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1.

eLife

Menzies SA,Volkmar N,van den Boomen DJ,Timms RT,Dickson AS,Nathan JA,Lehner PJ

Thu Dec 13 00:00:00 EST 2018

iRhom2 is essential for innate immunity to RNA virus by antagonizing ER- and mitochondria-associated degradation of VISA.

PLoS pathogens

Luo WW,Li S,Li C,Zheng ZQ,Cao P,Tong Z,Lian H,Wang SY,Shu HB,Wang YY

Wed Nov 01 00:00:00 EDT 2017

iRhom2 is essential for innate immunity to RNA virus by antagonizing ER- and mitochondria-associated degradation of VISA.

PLoS pathogens

Luo WW,Li S,Li C,Zheng ZQ,Cao P,Tong Z,Lian H,Wang SY,Shu HB,Wang YY

Wed Nov 01 00:00:00 EDT 2017

iRhom2 is essential for innate immunity to RNA virus by antagonizing ER- and mitochondria-associated degradation of VISA.

PLoS pathogens

Luo WW,Li S,Li C,Zheng ZQ,Cao P,Tong Z,Lian H,Wang SY,Shu HB,Wang YY

Wed Nov 01 00:00:00 EDT 2017

iRhom2 is essential for innate immunity to RNA virus by antagonizing ER- and mitochondria-associated degradation of VISA.

PLoS pathogens

Luo WW,Li S,Li C,Zheng ZQ,Cao P,Tong Z,Lian H,Wang SY,Shu HB,Wang YY

Wed Nov 01 00:00:00 EDT 2017

Aberrant corticosteroid metabolism in tumor cells enables GR takeover in enzalutamide resistant prostate cancer.

eLife

Li J,Alyamani M,Zhang A,Chang KH,Berk M,Li Z,Zhu Z,Petro M,Magi-Galluzzi C,Taplin ME,Garcia JA,Courtney K,Klein EA,Sharifi N

Mon Feb 13 00:00:00 EST 2017

Aberrant corticosteroid metabolism in tumor cells enables GR takeover in enzalutamide resistant prostate cancer.

eLife

Li J,Alyamani M,Zhang A,Chang KH,Berk M,Li Z,Zhu Z,Petro M,Magi-Galluzzi C,Taplin ME,Garcia JA,Courtney K,Klein EA,Sharifi N

Mon Feb 13 00:00:00 EST 2017

Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway.

Cell death & disease

Wang Y,Gao P,Wei C,Li H,Zhang L,Zhao Y,Wu B,Tian Y,Zhang W,Wu L,Wang R,Xu C

Thu May 18 00:00:00 EDT 2017

Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway.

Cell death & disease

Wang Y,Gao P,Wei C,Li H,Zhang L,Zhao Y,Wu B,Tian Y,Zhang W,Wu L,Wang R,Xu C

Thu May 18 00:00:00 EDT 2017

BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation.

Cell

Llambi F,Wang YM,Victor B,Yang M,Schneider DM,Gingras S,Parsons MJ,Zheng JH,Brown SA,Pelletier S,Moldoveanu T,Chen T,Green DR

Thu Apr 07 00:00:00 EDT 2016

BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation.

Cell

Llambi F,Wang YM,Victor B,Yang M,Schneider DM,Gingras S,Parsons MJ,Zheng JH,Brown SA,Pelletier S,Moldoveanu T,Chen T,Green DR

Thu Apr 07 00:00:00 EDT 2016

The E3 ubiquitin ligase AMFR and INSIG1 bridge the activation of TBK1 kinase by modifying the adaptor STING.

Immunity

Wang Q,Liu X,Cui Y,Tang Y,Chen W,Li S,Yu H,Pan Y,Wang C

Thu Dec 18 00:00:00 EST 2014

The E3 ubiquitin ligase AMFR and INSIG1 bridge the activation of TBK1 kinase by modifying the adaptor STING.

Immunity

Wang Q,Liu X,Cui Y,Tang Y,Chen W,Li S,Yu H,Pan Y,Wang C

Thu Dec 18 00:00:00 EST 2014

The E3 ubiquitin ligase AMFR and INSIG1 bridge the activation of TBK1 kinase by modifying the adaptor STING.

Immunity

Wang Q,Liu X,Cui Y,Tang Y,Chen W,Li S,Yu H,Pan Y,Wang C

Thu Dec 18 00:00:00 EST 2014

The E3 ubiquitin ligase AMFR and INSIG1 bridge the activation of TBK1 kinase by modifying the adaptor STING.

Immunity

Wang Q,Liu X,Cui Y,Tang Y,Chen W,Li S,Yu H,Pan Y,Wang C

Thu Dec 18 00:00:00 EST 2014

Bioinformatics

Protein Aliases: AMF receptor; Autocrine motility factor receptor; autocrine motility factor receptor, E3 ubiquitin protein ligase; E3 ubiquitin-protein ligase AMFR; gp78; RING finger protein 45; RING-type E3 ubiquitin transferase AMFR

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Gene Aliases: AMFR; GP78; RNF45

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UniProt ID: (Human) Q9UKV5, (Mouse) Q9R049

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Entrez Gene ID: (Human) 267, (Rat) 361367, (Mouse) 23802

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Function(s)

ubiquitin-protein transferase activity receptor activity protein binding zinc ion binding ligase activity protein binding, bridging ubiquitin-ubiquitin ligase activity chaperone binding ubiquitin protein ligase activity ubiquitin protein ligase activity involved in ERAD pathway BAT3 complex binding ubiquitin-specific protease binding molecular_function nucleotide binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity ubiquitin-protein ligase protein modifying enzyme

Process(es)

protein polyubiquitination ubiquitin-dependent protein catabolic process movement of cell or subcellular component signal transduction aging learning or memory ER-associated ubiquitin-dependent protein catabolic process endoplasmic reticulum unfolded protein response positive regulation of protein binding ERAD pathway protein ubiquitination involved in ubiquitin-dependent protein catabolic process protein oligomerization protein autoubiquitination protein K48-linked ubiquitination cellular process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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AMFR Polyclonal Antibody

AMFR Antibody (16675-1-AP) in ICC/IF

Product Details

16675-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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