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AMFR Antibody (PA5-96253) in WB
Western blot analysis of AMFR in extracts of various cell lines. Samples were incubated with AMFR Polyclonal antibody (Product # PA5-96253) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-96253
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 384-643 of human AMFR (NP_0011353)
Conjugate
Form
Liquid
Concentration
0.99 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808055
Product Specific Information
Immunogen sequence: IADNNRVREE HQGENLDENL VPVAAAEGRP RLNQHNHFFH FDGSRIASWL PSFSVEVMHT TNILGITQAS NSQLNAMAHQ IQEMFPQVPY HLVLQDLQLT RSVEITTDNI LEGRIQVPFP TQRSDSIRPA LNSPVERPSS DQEEGETSAQ TERVPLDLSP RLEETLDFGE VEVEPSEVED FEARGSRFSK SADERQRMLV QRKDELLQQA RKRFLNKSSE DDAASESFLP SEGASSDPVT LRRRMLAAAA ERRLQKQQTS; Positive Samples: SKOV3, HepG2, DU145; Cellular Location: Endoplasmic reticulum membrane, Multi-pass membrane protein
Target Information
Autocrine motility factor is a tumor motility-stimulating protein secreted by tumor cells. The protein encoded by this gene is a glycosylated transmembrane protein and a receptor for autocrine motility factor. The receptor, which shows some sequence similarity to tumor protein p53, is localized to the leading and trailing edges of carcinoma cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AMF receptor; Autocrine motility factor receptor; E3 ubiquitin-protein ligase AMFR; gp78; RING finger protein 45
Gene Aliases: AMFR; GP78; RNF45
UniProt ID: (Human) Q9UKV5
Entrez Gene ID: (Human) 267
ubiquitin-protein transferase activity
receptor activity
protein binding
zinc ion binding
ligase activity
protein binding, bridging
ubiquitin-ubiquitin ligase activity
chaperone binding
ubiquitin protein ligase activity
ubiquitin protein ligase activity involved in ERAD pathway
BAT3 complex binding
ubiquitin-specific protease binding
ubiquitin-protein ligase
protein modifying enzyme
protein polyubiquitination
ubiquitin-dependent protein catabolic process
movement of cell or subcellular component
signal transduction
aging
learning or memory
ER-associated ubiquitin-dependent protein catabolic process
endoplasmic reticulum unfolded protein response
positive regulation of protein binding
ERAD pathway
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
protein oligomerization
protein autoubiquitination
protein K48-linked ubiquitination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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