Invitrogen

ANP Polyclonal Antibody

6 Published Figures

8 References

View all (21) ANP antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (7)
Published Figures (6)

ANP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 13

ANP Antibody (PA5-29559) in IHC (P)

Immunohistochemistry (Paraffin) analysis of ANP was performed in paraffin-embedded mouse heart tissue using ANP Polyclonal Antibody (Product # PA5-29559) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

ANP Antibody in Immunohistochemistry (Frozen) (IHC (F))

ANP Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-29559

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:20,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full length human ANP Recombinant protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.43 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2547035

Product Specific Information

Recommended positive controls: mouse heart, rat heart.

Predicted reactivity: Mouse (80%), Rat (80%), Dog (85%), Pig (87%), Rabbit (81%), Bovine (85%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

RIP3 Contributes to Cardiac Hypertrophy by Influencing MLKL-Mediated Calcium Influx.

Oxidative medicine and cellular longevity

Xue H,Shi H,Zhang F,Li H,Li C,Han Q

Published figure using ANP polyclonal antibody (Product # PA5-29559) in Western Blot

Tue Apr 26 00:00:00 EDT 2022

A remarkable adaptive paradigm of heart performance and protection emerges in response to marked cardiac-specific overexpression of ADCY8.

eLife

Tarasov KV,Chakir K,Riordon DR,Lyashkov AE,Ahmet I,Perino MG,Silvester AJ,Zhang J,Wang M,Lukyanenko YO,Qu JH,Barrera MC,Juhaszova M,Tarasova YS,Ziman B,Telljohann R,Kumar V,Ranek M,Lammons J,Bychkov R,de Cabo R,Jun S,Keceli G,Gupta A,Yang D,Aon MA,Adamo L,Morrell CH,Otu W,Carroll C,Chambers S,Paolocci N,Huynh T,Pacak K,Weiss R,Field L,Sollott SJ,Lakatta EG

PA5-29559 was used in Western Blotting to show that TGAC8 mice show classical cardiac hypertrophy markers were absent in TGAC8, and that total left ventricular (LV) mass was not increased: a reduced LV cavity volume in TGAC8 was encased by thicker LV walls harboring an increased number of small cardiac myocytes, and a network of small interstitial proliferative non-cardiac myocytes compared to wild type (WT) littermates compared to wild type littermates.

Wed Dec 14 00:00:00 EST 2022

Vaspin Mediates the Intraorgan Crosstalk Between Heart and Adipose Tissue in Lipoatrophic Mice.

Frontiers in cell and developmental biology

Zhang D,Zhu H,Zhan E,Wang F,Liu Y,Xu W,Liu X,Liu J,Li S,Pan Y,Wang Y,Cao W

Published figure using ANP polyclonal antibody (Product # PA5-29559) in Western Blot

Tue Oct 12 00:00:00 EDT 2021

Vaspin Mediates the Intraorgan Crosstalk Between Heart and Adipose Tissue in Lipoatrophic Mice.

Frontiers in cell and developmental biology

Zhang D,Zhu H,Zhan E,Wang F,Liu Y,Xu W,Liu X,Liu J,Li S,Pan Y,Wang Y,Cao W

Published figure using ANP polyclonal antibody (Product # PA5-29559) in Western Blot

Tue Oct 12 00:00:00 EDT 2021

Atrial Natriuretic Peptide Promotes Neurite Outgrowth and Survival of Cochlear Spiral Ganglion Neurons in vitro Through NPR-A/cGMP/PKG Signaling.

Frontiers in cell and developmental biology

Sun F,Zhou K,Tian KY,Zhang XY,Liu W,Wang J,Zhong CP,Qiu JH,Zha DJ

PA5-29559 was used in Immunohistochemistry (Frozen) to study how atrial natriuretic peptide (ANP) could support and attract neurite outgrowth of spiral ganglion neurons (SGNs) and possess a high capacity to improve neuronal survival of SGNs against glutamate-induced excitotoxicity by triggering the natriuretic peptide receptor (NPR-A)/cyclic guanosine-3',5'-monophosphate (cGMP)/PKG pathway.

Sat Jul 17 00:00:00 EDT 2021

Atrial Natriuretic Peptide Promotes Neurite Outgrowth and Survival of Cochlear Spiral Ganglion Neurons in vitro Through NPR-A/cGMP/PKG Signaling.

Frontiers in cell and developmental biology

Sun F,Zhou K,Tian KY,Zhang XY,Liu W,Wang J,Zhong CP,Qiu JH,Zha DJ

Sat Jul 17 00:00:00 EDT 2021

Natriuretic Peptides Regulate Prostate Cells Inflammatory Behavior: Potential Novel Anticancer Agents for Prostate Cancer.

Biomolecules

Mezzasoma L,Talesa VN,Costanzi E,Bellezza I

PA5-29559 was used in Western Blot to analyze the contribution of both Atrial Natriuretic Peptides (ANP) and B-Type Natriuretic Peptide (BNP) in the regulation of the inflammatory behavior in prostate cancerous and non-cancerous cell lines.

Tue May 25 00:00:00 EDT 2021

Natriuretic Peptides Regulate Prostate Cells Inflammatory Behavior: Potential Novel Anticancer Agents for Prostate Cancer.

Biomolecules

Mezzasoma L,Talesa VN,Costanzi E,Bellezza I

Tue May 25 00:00:00 EDT 2021

Atrial Natriuretic Peptide Improves Neurite Outgrowth from Spiral Ganglion Neurons In Vitro through a cGMP-Dependent Manner.

Neural plasticity

Sun F,Zhou K,Tian KY,Wang J,Qiu JH,Zha DJ

PA5-29559 was used in Immunohistochemistry to investigate the expression patterns of ANP and its receptors in the cochlear SG and dissociated SGNs and determined the influence of ANP on neurite outgrowth in vitro by using organotypic SG explants and dissociated SGN cultures from postnatal rats.

Wed Sep 29 00:00:00 EDT 2021

Atrial Natriuretic Peptide Improves Neurite Outgrowth from Spiral Ganglion Neurons In Vitro through a cGMP-Dependent Manner.

Neural plasticity

Sun F,Zhou K,Tian KY,Wang J,Qiu JH,Zha DJ

Wed Sep 29 00:00:00 EDT 2021

Cardiac specific PRMT1 ablation causes heart failure through CaMKII dysregulation.

Nature communications

Pyun JH,Kim HJ,Jeong MH,Ahn BY,Vuong TA,Lee DI,Choi S,Koo SH,Cho H,Kang JS

PA5-29559 was used in Western Blotting to suggest that PRMT1 is a critical regulator of CaMKII to maintain cardiac function.

Fri Nov 30 00:00:00 EST 2018

Bioinformatics

Protein Aliases: atrial natriuretic factor; Atrial natriuretic factor prohormone; atrial natriuretic peptide; Atrial natriuretic peptide prohormone; Atriopeptigen; atriopeptin; Cardiodilatin; cardiodilatin-related peptide; cardionatrin; CDD; Natriuretic peptide precursor A (pronatriodilatin, also Anf, Pnd); natriuretic peptide precursor A variant 1; natriuretic peptide precursor A variant NPPA-M1; natriuretic peptide precursor A variant NPPA-M2; natriuretic peptide precursor A variant NPPA-M3; natriuretic peptide precursor type A; Natriuretic peptides A; preproANF; preproANP; preproCDD-ANF; prepronatriodilatin; proANF; Pronatriodilatin; urodilatin

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Gene Aliases: ANF; ANP; ATFB6; ATRST2; CDD; CDD-ANF; CDP; NPPA; PND; RATANF

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UniProt ID: (Human) P01160, (Rat) P01161, (Mouse) P05125

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Entrez Gene ID: (Human) 4878, (Rat) 24602, (Mouse) 230899

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Function(s)

receptor binding neuropeptide hormone activity protein binding peptide hormone receptor binding neuropeptide receptor binding hormone activity

Process(es)

response to hypoxia negative regulation of systemic arterial blood pressure cGMP biosynthetic process transcription initiation from RNA polymerase II promoter receptor guanylyl cyclase signaling pathway neuropeptide signaling pathway female pregnancy regulation of blood pressure positive regulation of heart rate cardiac muscle hypertrophy in response to stress negative regulation of cell growth response to insulin response to muscle stretch cellular protein metabolic process regulation of blood vessel size regulation of atrial cardiac muscle cell membrane repolarization positive regulation of cardiac muscle contraction cell growth involved in cardiac muscle cell development cellular response to mechanical stimulus regulation of high voltage-gated calcium channel activity positive regulation of delayed rectifier potassium channel activity regulation of calcium ion transmembrane transport via high voltage-gated calcium channel positive regulation of potassium ion export across plasma membrane regulation of cardiac conduction negative regulation of collecting lymphatic vessel constriction heart development regulation of body fluid levels multicellular organismal water homeostasis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ANP Polyclonal Antibody

ANP Antibody (PA5-29559) in IHC (P)

Product Details

PA5-29559

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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