Proteintech

ANP32B Polyclonal Antibody

ANP32B Antibody in Immunocytochemistry (ICC/IF)

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ANP32B Antibody (10843-1-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 10843-1-AP (ANP32B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ANP32B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ANP32B Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10843-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ANP32B Fusion Protein Ag1274 (1-251 aa encoded by BC013003)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MDMKRRIHL ELRNRTPAAV RELVLDNCKS NDGKIEGLTA EFVNLEFLSL INVGLISVSN LPKLPKLKKL ELSENRIFGG LDMLAEKLPN LTHLNLSGNK LKDISTLEPL KKLECLKSLD LFNCEVTNLN DYRESVFKLL PQLTYLDGYD REDQEAPDSD AEVDGVDEEE EDEEGEDEED EDDEDGEEEE FDEEDDEDED VEGDEDDDEV SEEEEEFGLD EEDEDEDEDE EEEEGGKGEK RKRETDDEGE DD (1-251 aa encoded by BC013003)

Target Information

Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Epigallocatechin gallate (EGCG) attenuates myocardial hypertrophy and fibrosis induced by transverse aortic constriction via inhibiting the Akt/mTOR pathway.

Pharmaceutical biology

Cui Y,Wang Y,Liu G

Wed Dec 01 00:00:00 EST 2021

A natural variant in ANP32B impairs influenza virus replication in human cells.

The Journal of general virology

Staller E,Sheppard CM,Baillon L,Frise R,Peacock TP,Sancho-Shimizu V,Barclay WS

Wed Sep 01 00:00:00 EDT 2021

ANP32 Proteins Are Essential for Influenza Virus Replication in Human Cells.

Journal of virology

Staller E,Sheppard CM,Neasham PJ,Mistry B,Peacock TP,Goldhill DH,Long JS,Barclay WS

Sun Sep 01 00:00:00 EDT 2019

ANP32B deficiency impairs proliferation and suppresses tumor progression by regulating AKT phosphorylation.

Cell death & disease

Yang S,Zhou L,Reilly PT,Shen SM,He P,Zhu XN,Li CX,Wang LS,Mak TW,Chen GQ,Yu Y

Thu Feb 04 00:00:00 EST 2016

ANP32B deficiency impairs proliferation and suppresses tumor progression by regulating AKT phosphorylation.

Cell death & disease

Yang S,Zhou L,Reilly PT,Shen SM,He P,Zhu XN,Li CX,Wang LS,Mak TW,Chen GQ,Yu Y

Thu Feb 04 00:00:00 EST 2016

ANP32B deficiency impairs proliferation and suppresses tumor progression by regulating AKT phosphorylation.

Cell death & disease

Yang S,Zhou L,Reilly PT,Shen SM,He P,Zhu XN,Li CX,Wang LS,Mak TW,Chen GQ,Yu Y

Thu Feb 04 00:00:00 EST 2016

ANP32B deficiency impairs proliferation and suppresses tumor progression by regulating AKT phosphorylation.

Cell death & disease

Yang S,Zhou L,Reilly PT,Shen SM,He P,Zhu XN,Li CX,Wang LS,Mak TW,Chen GQ,Yu Y

Thu Feb 04 00:00:00 EST 2016

Caspase-3-resistant uncleavable form of acidic leucine-rich nuclear phosphoprotein 32B potentiates leukemic cell apoptosis.

Molecular medicine reports

Li CX,Shen SM,Wang LS,Yu Y

Wed Apr 01 00:00:00 EDT 2015

Caspase-3-resistant uncleavable form of acidic leucine-rich nuclear phosphoprotein 32B potentiates leukemic cell apoptosis.

Molecular medicine reports

Li CX,Shen SM,Wang LS,Yu Y

Wed Apr 01 00:00:00 EDT 2015

Synergistic cytotoxicity of gemcitabine, clofarabine and edelfosine in lymphoma cell lines.

Blood cancer journal

Valdez BC,Zander AR,Song G,Murray D,Nieto Y,Li Y,Champlin RE,Andersson BS

Fri Jan 10 00:00:00 EST 2014

Synergistic cytotoxicity of gemcitabine, clofarabine and edelfosine in lymphoma cell lines.

Blood cancer journal

Valdez BC,Zander AR,Song G,Murray D,Nieto Y,Li Y,Champlin RE,Andersson BS

Fri Jan 10 00:00:00 EST 2014

Acidic leucine-rich nuclear phosphoprotein 32 family member B (ANP32B) contributes to retinoic acid-induced differentiation of leukemic cells.

Biochemical and biophysical research communications

Yu Y,Shen SM,Zhang FF,Wu ZX,Han B,Wang LS

Fri Jul 13 00:00:00 EDT 2012

Acidic leucine-rich nuclear phosphoprotein 32 family member B (ANP32B) contributes to retinoic acid-induced differentiation of leukemic cells.

Biochemical and biophysical research communications

Yu Y,Shen SM,Zhang FF,Wu ZX,Han B,Wang LS

Fri Jul 13 00:00:00 EDT 2012

Acidic nuclear phosphoprotein 32kDa (ANP32)B-deficient mouse reveals a hierarchy of ANP32 importance in mammalian development.

Proceedings of the National Academy of Sciences of the United States of America

Reilly PT,Afzal S,Gorrini C,Lui K,Bukhman YV,Wakeham A,Haight J,Ling TW,Cheung CC,Elia AJ,Turner PV,Mak TW

Tue Jun 21 00:00:00 EDT 2011

Acidic nuclear phosphoprotein 32kDa (ANP32)B-deficient mouse reveals a hierarchy of ANP32 importance in mammalian development.

Proceedings of the National Academy of Sciences of the United States of America

Reilly PT,Afzal S,Gorrini C,Lui K,Bukhman YV,Wakeham A,Haight J,Ling TW,Cheung CC,Elia AJ,Turner PV,Mak TW

Tue Jun 21 00:00:00 EDT 2011

Downregulation of ANP32B, a novel substrate of caspase-3, enhances caspase-3 activation and apoptosis induction in myeloid leukemic cells.

Carcinogenesis

Shen SM,Yu Y,Wu YL,Cheng JK,Wang LS,Chen GQ

Mon Mar 01 00:00:00 EST 2010

Downregulation of ANP32B, a novel substrate of caspase-3, enhances caspase-3 activation and apoptosis induction in myeloid leukemic cells.

Carcinogenesis

Shen SM,Yu Y,Wu YL,Cheng JK,Wang LS,Chen GQ

Mon Mar 01 00:00:00 EST 2010

Generation and characterization of the Anp32e-deficient mouse.

PloS one

Reilly PT,Afzal S,Wakeham A,Haight J,You-Ten A,Zaugg K,Dembowy J,Young A,Mak TW

Tue Oct 26 00:00:00 EDT 2010

Generation and characterization of the Anp32e-deficient mouse.

PloS one

Reilly PT,Afzal S,Wakeham A,Haight J,You-Ten A,Zaugg K,Dembowy J,Young A,Mak TW

Tue Oct 26 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: acidic (leucine-rich) nuclear phosphoprotein 32 family, member B; Acidic leucine-rich nuclear phosphoprotein 32 family member B; acidic nuclear phosphoprotein 32 family, member B; Acidic protein rich in leucines; OTTHUMP00000063770; PHAPI2; proliferation related acidic leucine rich protein PAL31; Proliferation-related acidic leucine-rich protein PAL31; Putative HLA-DR-associated protein I-2; Silver-stainable protein SSP29; TNF superfamily member 13

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Gene Aliases: 2410015B15Rik; ANP32B; APRIL; PAL31; PHAPI2; PHAPI2a; SSP29

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UniProt ID: (Human) Q92688, (Mouse) Q9EST5

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Entrez Gene ID: (Human) 10541, (Mouse) 67628

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Function(s)

protein binding histone binding RNA polymerase binding chromatin/chromatin-binding, or -regulatory protein

Process(es)

vasculature development nucleosome assembly activation of cysteine-type endopeptidase activity involved in apoptotic process ventricular system development negative regulation of cell differentiation positive regulation of protein export from nucleus inner ear development palate development G1/S transition of mitotic cell cycle positive regulation of cell proliferation positive regulation of G1/S transition of mitotic cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ANP32B Polyclonal Antibody

ANP32B Antibody (10843-1-AP) in ICC/IF

Product Details

10843-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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