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ANP32E Antibody (PA5-116737) in WB
Western blot analysis of ANP32E in extracts of various cell lines. Samples were incubated with ANP32E Polyclonal antibody (Product # PA5-116737) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-116737
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human ANP32E (NP_1121821)
Conjugate
Form
Liquid
Concentration
1.05 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2901368
Product Specific Information
Positive Samples: K-562, HeLa
Immunogen sequence: MEMKKKINLE LRNRSPEEVT ELVLDNCLCV NGEIEGLNDT FKELEFLSMA NVELSSLARL PSLNKLRKLE
Target Information
Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acid nuclear phosphoprotein 32 (leucine rich); acidic (leucine-rich) nuclear phosphoprotein 32 family, member A; acidic (leucine-rich) nuclear phosphoprotein 32 family, member B; acidic (leucine-rich) nuclear phosphoprotein 32 family, member E; Acidic leucine-rich nuclear phosphoprotein 32 family member A; Acidic leucine-rich nuclear phosphoprotein 32 family member B; Acidic leucine-rich nuclear phosphoprotein 32 family member E; acidic nuclear phosphoprotein 32; acidic nuclear phosphoprotein 32 family, member A; acidic nuclear phosphoprotein 32 family, member B; Acidic nuclear phosphoprotein pp32; Acidic protein rich in leucines; cerebellar leucine rich acidic nuclear protein; Cerebellar postnatal development protein 1; hepatopoietin Cn; inhibitor-1 of protein phosphatase-2A; LANP; LANP-L; LANP-like protein; Leucine-rich acidic nuclear protein; leucine-rich acidic nuclear protein LANP; leucine-rich acidic nuclear protein like; Mapmodulin; PHAPI; PHAPI2; Potent heat-stable protein phosphatase 2A inhibitor I1PP2A; pp32; proliferation related acidic leucine rich protein PAL31; Proliferation-related acidic leucine-rich protein PAL31; Putative HLA-DR-associated protein I; Putative HLA-DR-associated protein I-2; putative human HLA class II associated protein I; Silver-stainable protein SSP29; vacidic (leucine-rich) nuclear phosphoprotein 32 family, member A
Gene Aliases: 2410015B15Rik; 2810018A15Rik; AI047746; AI326868; Anp32; ANP32A; ANP32B; ANP32E; APRIL; C15orf1; CPD1; HPPCn; I1PP2A; LANP; LANP-L; LANPL; MAPM; mLANP-L; PAL31; PHAP1; PHAPI; PHAPI2; PHAPI2a; PP32; SSP29; W91701
UniProt ID: (Human) P39687, (Human) Q92688, (Human) Q9BTT0, (Rat) P49911, (Mouse) O35381, (Mouse) Q9EST5, (Rat) Q9EST6, (Mouse) P97822, (Rat) Q5XIE0
Entrez Gene ID: (Human) 8125, (Human) 10541, (Human) 81611, (Rat) 25379, (Mouse) 11737, (Mouse) 67628, (Rat) 170724, (Mouse) 66471, (Rat) 361999
transcription, DNA-templated
regulation of transcription, DNA-templated
nucleocytoplasmic transport
intracellular signal transduction
regulation of mRNA stability
vasculature development
nucleosome assembly
activation of cysteine-type endopeptidase activity involved in apoptotic process
ventricular system development
negative regulation of cell differentiation
positive regulation of protein export from nucleus
inner ear development
palate development
covalent chromatin modification
negative regulation of catalytic activity
histone exchange
G1/S transition of mitotic cell cycle
positive regulation of cell proliferation
positive regulation of G1/S transition of mitotic cell cycle
chromatin modification
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