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AP1G1 Antibody (600-101-289) in WB
Western blot using Rocklands Affinity Purified anti-AP1G1 antibody shows strong detection of a 91-kDa band corresponding to Human AP1G1 in a HeLa whole cell lysate (lane 1). Peptide competition (using 1 µg/ml of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (lane 2). Approximately 20 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP1G1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG ... View More
Product Details
600-101-289
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 625-675 of Human AP1G1.
Conjugate
Form
Liquid
Concentration
0.7 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human AP1G1. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and orangutan based on 100% homology for the immunogen sequence. Cross reactivity with AP1G1 protein from mouse, rat, dog and chicken may occur as sequence homology varies by only one amino acid residue in this sequence as indicated by BLAST analysis. Reactivity with AP1G1 proteins from other sources is not known.
Target Information
Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adapter-related protein complex 1 subunit gamma-1; Adaptor protein complex AP-1 subunit gamma-1; adaptor-related protein complex 1 gamma 1 subunit; Adaptor-related protein complex 1 subunit gamma-1; adaptor-related protein complex 1, gamma 1 subunit; AP-1 complex subunit gamma-1; clathrin assembly protein complex 1 gamma large chain; Clathrin assembly protein complex 1 gamma-1 large chain; clathrin-associated/assembly/adaptor protein, large, gamma 1; gamma adaptin; Gamma1-adaptin; golgi adaptor HA1/AP1 adaptin gamma subunit; Golgi adaptor HA1/AP1 adaptin subunit gamma-1; MGC18255; testicular tissue protein Li 21
Gene Aliases: ADTG; AP1G1; CLAPG1
UniProt ID: (Human) O43747
Entrez Gene ID: (Human) 164
intracellular protein transport
antigen processing and presentation of exogenous peptide antigen via MHC class II
melanosome organization
endosome to melanosome transport
positive regulation of natural killer cell degranulation
positive regulation of natural killer cell mediated cytotoxicity
regulation of defense response to virus by virus
Golgi to lysosome transport
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