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Product Details
H00000317-B01P
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
APAF1 (NP_863658.1, 1 a.a. approximately 1205 a.a) full-length human protein.
Conjugate
Form
Liquid
Concentration
0.5-1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Product may be used with Western Blot (Transfected lysate).
The Isotype of this product is composed of an IgG Mixture.
Immunogen sequence: MDAKARNCLL QHREALEKDI KTSYIMDHMI SDGFLTISEE EKVRNEPTQQ QRAAMLIKMI LKKDNDSYVS FYNALLHEGY KDLAALLHDG IPVVSSSSGK DSVSGITSYV RTVLCEGGVP QRPVVFVTRK KLVNAIQQKL SKLKGEPGWV TIHGMAGCGK SVLAAEAVRD HSLLEGCFPG GVHWVSVGKQ DKSGLLMKLQ NLCTRLDQDE SFSQRLPLNI EEAKDRLRIL MLRKHPRSLL ILDDVWDSWV LKAFDSQCQI LLTTRDKSVT DSVMGPKYVV PVESSLGKEK GLEILSLFVN MKKADLPEQA HSIIKECKGS PLVVSLIGAL LRDFPNRWEY YLKQLQNKQF KRIRKSSSYD YEALDEAMSI SVEMLREDIK DYYTDLSILQ KDVKVPTKVL CILWDMETEE VEDILQEFVN KSLLFCDRNG KSFRYYLHDL QVDFLTEKNC SQLQDLHKKI ITQFQRYHQP HTLSPDQEDC MYWYNFLAYH MASAKMHKEL CALMFSLDWI KAKTELVGPA HLIHEFVEYR HILDEKDCAV SENFQEFLSL NGHLLGRQPF PNIVQLGLCE PETSEVYQQA KLQAKQEVDN GMLYLEWINK KNITNLSRLV VRPHTDAVYH ACFSEDGQRI ASCGADKTLQ VFKAETGEKL LEIKAHEDEV LCCAFSTDDR FIATCSVDKK VKIWNSMTGE LVHTYDEHSE QVNCCHFTNS SHHLLLATGS SDCFLKLWDL NQKECRNTMF GHTNSVNHCR FSPDDKLLAS CSADGTLKLW DATSANERKS INVKQFFLNL EDPQEDMEVI VKCCSWSADG ARIMVAAKNK IFLWNTDSRS KVADCRGHLS WVHGVMFSPD GSSFLTSSDD QTIRLWETKK VCKNSAVMLK QEVDVVFQEN EVMVLAVDHI RRLQLINGRT GQIDYLTEAQ VSCCCLSPHL QYIAFGDENG AIEILELVNN RIFQSRFQHK KTVWHIQFTA DEKTLISSSD DAEIQVWNWQ LDKCIFLRGH QETVKDFRLL KNSRLLSWSF DGTVKVWNII TGNKEKDFVC HQGTVLSCDI SHDATKFSST SADKTAKIWS FDLLLPLHEL RGHNGCVRCS AFSVDSTLLA TGDDNGEIRI WNVSNGELLH LCAPLSEEGA ATHGGWVTDL CFSPDGKMLI SAGGYIKWWN VVTGESSQTF YTNGTNLKKI HVSPDFKTYV TVDNLGILYI LQTLE
Target Information
APAF1 gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APAF-1; Apoptotic protease-activating factor 1; DKFZp781B1145
Gene Aliases: APAF-1; APAF1; CED4; KIAA0413
UniProt ID: (Human) O14727
Entrez Gene ID: (Human) 317
response to hypoxia
neural tube closure
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
nervous system development
aging
response to nutrient
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
cardiac muscle cell apoptotic process
cell differentiation
forebrain development
glial cell apoptotic process
regulation of apoptotic process
positive regulation of apoptotic process
protein homooligomerization
neuron apoptotic process
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to transforming growth factor beta stimulus
response to G1 DNA damage checkpoint signaling
intrinsic apoptotic signaling pathway
regulation of apoptotic DNA fragmentation
positive regulation of apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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