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Product Details
PA5-120054
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 470-600 of human APAF1 (NP_863651.1).
Conjugate
Form
Liquid
Concentration
1.45 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2913626
Product Specific Information
Positive test controls include: Jurkat, 293T, Mouse testis. The target is usually found in the following locations: Cytoplasm.
Immunogen sequence: PHTLSPDQED CMYWYNFLAY HMASAKMHKE LCALMFSLDW IKAKTELVGP AHLIHEFVEY RHILDEKDCA VSENFQEFLS LNGHLLGRQP FPNIVQLGLC EPETSEVYQQ AKLQAKQEVD NGMLYLEWIN K
Target Information
APAF1 gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APAF-1; apoptotic protease activating factor 1; Apoptotic protease-activating factor 1; DKFZp781B1145; forebrain overgrowth
Gene Aliases: 6230400I06Rik; APAF-1; APAF1; Apaf1l; CED4; fog; KIAA0413; mKIAA0413
UniProt ID: (Human) O14727, (Rat) Q9EPV5, (Mouse) O88879
Entrez Gene ID: (Human) 317, (Rat) 78963, (Mouse) 11783
response to hypoxia
neural tube closure
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
multicellular organism development
brain development
aging
response to nutrient
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
cardiac muscle cell apoptotic process
cell differentiation
forebrain development
glial cell apoptotic process
protein homooligomerization
neuron apoptotic process
cellular response to transforming growth factor beta stimulus
apoptotic signaling pathway
regulation of apoptotic DNA fragmentation
positive regulation of apoptotic signaling pathway
nervous system development
regulation of apoptotic process
positive regulation of apoptotic process
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
response to G1 DNA damage checkpoint signaling
intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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