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Product Details
PA5-19893
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A peptide corresponding to amino acids near the amino terminus of human Apaf1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11152742
Product Specific Information
In Western blot applications, this antibody detects a band at ~115-130kDa. A suggested positive control is K562 cell lysate.
Target Information
APAF1 gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APAF-1; apoptotic protease activating factor 1; Apoptotic protease-activating factor 1; DKFZp781B1145; forebrain overgrowth
Gene Aliases: 6230400I06Rik; APAF-1; APAF1; Apaf1l; CED4; fog; KIAA0413; mKIAA0413
UniProt ID: (Human) O14727, (Rat) Q9EPV5, (Mouse) O88879
Entrez Gene ID: (Human) 317, (Rat) 78963, (Mouse) 11783
response to hypoxia
neural tube closure
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
multicellular organism development
brain development
aging
response to nutrient
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
cardiac muscle cell apoptotic process
cell differentiation
forebrain development
glial cell apoptotic process
protein homooligomerization
neuron apoptotic process
cellular response to transforming growth factor beta stimulus
apoptotic signaling pathway
regulation of apoptotic DNA fragmentation
positive regulation of apoptotic signaling pathway
nervous system development
regulation of apoptotic process
positive regulation of apoptotic process
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
response to G1 DNA damage checkpoint signaling
intrinsic apoptotic signaling pathway
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