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Invitrogen
APC Monoclonal Antibody (eBioAPC-6A2), eBioscience™
FIGURE: 1 / 1
APC Antibody (14-4170-81) in Flow
Staining of normal human peripheral blood cells with Anti-Human/Mouse CD44 APC (Product # 17-0441-82) followed by 0.015 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (left) or 0.015 µg of Anti-Allophycocyanin Purified (right) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Total viable cells were used for analysis.
Product Details
14-4170-81
Product Specifications
Species Reactivity
Tag
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
eBioAPC-6A2
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_657508
Product Specific Information
Description: The eBioAPC-6A2 monoclonal antibody reacts with allophycocyanin (APC) and APC-Cy7, with no crossreactivity to fluorescein (FITC) or phycoerythrin (PE). When using eBioAPC-6A2 as a second-step antibody, in combination with an appropriate third step, to bind a APC-conjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated APC antibody. Therefore no interference or quenching of the APC fluorochrome is observed. The eBioAPC-6A2 can be used for separation of cells labeled with APC-conjugated antibodies or for staining.
Please note that the optimal concentration for this antibody will vary depending on the density of the primary APC-conjugated antibody and the application tested.
Applications Reported: This eBioAPC-6A2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioAPC-6A2 antibody has been tested flow cytometric analysis of normal human peripheral blood cells stained with an APC-conjugated antibody. This can be used at less than or equal to by µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Anti-APC (Allophycocyanin) antibodies can be used to bind APC-conjugated primary antibodies without decreasing fluorescent intensity of the APC fluorochrome. This method is used for separation of cells labeled with APC-conjugated antibodies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APC
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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