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Product Details
PA5-85228
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human APE1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2792371
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (97%), Rat (96%), Dog (98%), Pig (97%), Rhesus Monkey (98%), Chimpanzee (99%), Bovine (97%).
Positive Control: A431, mouse brain, HeLa.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is a multifunctional, bipartite enzyme that plays an important role in numerous, cellular functions. APE is responsible for repairing abasic sites in DNA and in regulating the redox state of other proteins that play roles in oxidative signaling, transcription factor regulation (Fos, Jun, NF-kB, Myb, HIF-1 alpha, CREB, Pax), cell cycle control (p53), and apoptosis. The most common form of DNA damage is the creation of abasic sites which are brought about through spontaneous loss or oxidative DNA damage, through chemically initiated hydrolysis (chemotherapy), ionizing radiation, UV irradiation, oxidizing agents, and removal of modified bases by DNA glycosylases. APE is differentially expressed during development and in different tissues. This protein has diverse subcellular localization patterns which support the possibility of its interaction with numerous, other cellular proteins in addition to DNA repair within the nucleus. Regulation of APE by phosphorylation is mediated, at least in part, by casein kinase II. Increases in APE message and protein levels are observed upon the reintroduction of oxygen to hypoxic cells, and in some malignant tissue relative to normal tissue. Decreases in APE expression have been associated with the induction of cellular apoptosis.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AP endonuclease 1; AP endonuclease class I; AP lyase; APEN; APEX nuclease; APEX nuclease (multifunctional DNA repair enzyme) 1; Apurinic-apyrimidinic endonuclease 1; apurinic/a; Apurinic/apy; apurinic/apyrimidinic (abasic) endonuclease; deoxyribonuclease (apurinic or apyrimidinic); DNA repair nuclease/redox regulator APEX1; DNA-(apurinic or apyrimidinic site) endonuclease; DNA-(apurinic or apyrimidinic site) lyase; protein REF-1; redox factor 1; Redox factor-1; REF-1
Gene Aliases: APE; APE1; APEN; APEX; APEX1; APX; HAP1; Ref-1; REF1
UniProt ID: (Human) P27695, (Mouse) P28352
Entrez Gene ID: (Human) 328, (Mouse) 11792
DNA binding
damaged DNA binding
double-stranded telomeric DNA binding
transcription coactivator activity
transcription corepressor activity
DNA-(apurinic or apyrimidinic site) lyase activity
endonuclease activity
endodeoxyribonuclease activity
RNA-DNA hybrid ribonuclease activity
phosphodiesterase I activity
uracil DNA N-glycosylase activity
protein binding
phosphoric diester hydrolase activity
double-stranded DNA exodeoxyribonuclease activity
double-stranded DNA 3'-5' exodeoxyribonuclease activity
3'-5' exonuclease activity
oxidoreductase activity
site-specific endodeoxyribonuclease activity, specific for altered base
chromatin DNA binding
protein complex binding
poly(A) RNA binding
metal ion binding
NF-kappaB binding
RNA binding
nuclease activity
endoribonuclease activity
exonuclease activity
hydrolase activity
lyase activity
phosphoric ester hydrolase activity
T/G mismatch-specific endonuclease activity
retroviral 3' processing activity
DNA repair
base-excision repair
DNA recombination
transcription, DNA-templated
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
aging
negative regulation of smooth muscle cell migration
regulation of mRNA stability
cell redox homeostasis
positive regulation of DNA repair
oxidation-reduction process
cellular response to hydrogen peroxide
DNA demethylation
nucleic acid phosphodiester bond hydrolysis
RNA phosphodiester bond hydrolysis, endonucleolytic
positive regulation of G1/S transition of mitotic cell cycle
regulation of nucleic acid-templated transcription
telomere maintenance
base-excision repair, base-free sugar-phosphate removal
response to drug
cellular response to cAMP
cellular response to peptide hormone stimulus
negative regulation of nucleic acid-templated transcription
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