Invitrogen

APE1 Monoclonal Antibody (13B 8E5C2)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

5 Published Figures

3 References

View all (39) APE1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

APE1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 21

APE1 Antibody (MA1-440) in ICC/IF

Immunofluorescent analysis of APE1 (green) showing staining in the in the nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an APE1 monoclonal antibody (Product # MA1-440) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conju... View More

APE1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

APE1 Antibody in Immunohistochemistry (IHC)

APE1 Antibody in Immunoprecipitation (IP)

APE1 Antibody in RNA Immunoprecipitation (RIP)

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APE1 Antibody — The specificity of anti-APE1 mouse monoclonal antibody (Product # MA1-440) was demonstrated by CRISPR targeted APE1 knockout in 293T cells. Western blot analysis of whole cell lysates using this antibody showed no detection of APE1 protein expression in knockout cells compared to the APE1 protein detected at ~36kDa in wild-type 293T cells. {KO}

Product Details

MA1-440

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

1/5000

Immunocytochemistry (ICC/IF)

1:50-1:500

- -

Flow Cytometry (Flow)

2-5 µg/mL

Immunoprecipitation (IP)

5 µg

ChIP assay (ChIP)

1.5 µg/10^6 cells

RNA Immunoprecipitation (RIP)

Assay-dependent

Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

13B 8E5C2

Immunogen

Full-length recombinant human APE protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325426

Product Specific Information

MA1-440 detects apurinic/apyrimidinic endonuclease (APE/ref-1) from human, non human primate, canine, mouse and rat samples.

MA1-440 has been successfully used in Western blot, immunohistochemical, flow cytometry and immunoprecipitation procedures. By Western blot, this antibody detects a 36 kDa protein representing APE/ref-1 in HeLa cell lysate. Immunohistochemical staining of APE/ref-1 in a variety of normal and cancerous human tissues, including ovaries, cervix, prostate, germ cell tumors, osteosarcomas, and rhabdosarcomas, with MA1-440 yields a staining pattern consistent with nuclear staining.

The MA1-440 immunogen is full-length, recombinant human APE protein.

Target Information

Mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is a multifunctional, bipartite enzyme that plays an important role in numerous, cellular functions. APE is responsible for repairing abasic sites in DNA and in regulating the redox state of other proteins that play roles in oxidative signaling, transcription factor regulation (Fos, Jun, NF-kB, Myb, HIF-1 alpha, CREB, Pax), cell cycle control (p53), and apoptosis. The most common form of DNA damage is the creation of abasic sites which are brought about through spontaneous loss or oxidative DNA damage, through chemically initiated hydrolysis (chemotherapy), ionizing radiation, UV irradiation, oxidizing agents, and removal of modified bases by DNA glycosylases. APE is differentially expressed during development and in different tissues. This protein has diverse subcellular localization patterns which support the possibility of its interaction with numerous, other cellular proteins in addition to DNA repair within the nucleus. Regulation of APE by phosphorylation is mediated, at least in part, by casein kinase II. Increases in APE message and protein levels are observed upon the reintroduction of oxygen to hypoxic cells, and in some malignant tissue relative to normal tissue. Decreases in APE expression have been associated with the induction of cellular apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

APE1 redox function is required for activation of Yes-associated protein 1 under reflux conditions in Barrett's-associated esophageal adenocarcinomas.

Journal of experimental & clinical cancer research : CR

Ballout F,Lu H,Chen L,Sriramajayam K,Que J,Meng Z,Wang TC,Giordano S,Zaika A,McDonald O,Peng D,El-Rifai W

MA1-440 was used in Western Blotting to establish a novel function of APE1 in EAC progression elucidating druggable molecular vulnerabilities via targeting APE1 or YAP1 for the treatment of EAC.

Thu Sep 01 00:00:00 EDT 2022

APE1 redox function is required for activation of Yes-associated protein 1 under reflux conditions in Barrett's-associated esophageal adenocarcinomas.

Journal of experimental & clinical cancer research : CR

Ballout F,Lu H,Chen L,Sriramajayam K,Que J,Meng Z,Wang TC,Giordano S,Zaika A,McDonald O,Peng D,El-Rifai W

MA1-440 was used in Western Blotting to establish a novel function of APE1 in EAC progression elucidating druggable molecular vulnerabilities via targeting APE1 or YAP1 for the treatment of EAC.

Thu Sep 01 00:00:00 EDT 2022

Endogenous DNA 3' Blocks Are Vulnerabilities for BRCA1 and BRCA2 Deficiency and Are Reversed by the APE2 Nuclease.

Molecular cell

Álvarez-Quilón A,Wojtaszek JL,Mathieu MC,Patel T,Appel CD,Hustedt N,Rossi SE,Wallace BD,Setiaputra D,Adam S,Ohashi Y,Melo H,Cho T,Gervais C,Muñoz IM,Grazzini E,Young JTF,Rouse J,Zinda M,Williams RS,Durocher D

MA1-440 was used in Western Blot to conclude that the main role of APE2 is to reverse blocked 3' DNA ends, problematic lesions that preclude DNA synthesis.

Thu Jun 18 00:00:00 EDT 2020

Studies of apurinic/apyrimidinic endonuclease/ref-1 expression in epithelial ovarian cancer: correlations with tumor progression and platinum resistance.

Clinical cancer research : an official journal of the American Association for Cancer Research

Freitas S,Moore DH,Michael H,Kelley MR

MA1-440 was used in immunohistochemistry to investigate the correlations of apurinic/apyrimidinic endonuclease/ref-1 expression with tumor progression and platinum resistance in epithelial ovarian cancer

Wed Oct 15 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: AP endonuclease 1; AP endonuclease class I; AP lyase; APEN; APEX nuclease; APEX nuclease (multifunctional DNA repair enzyme) 1; APEX nuclease 1; Apurinic-apyrimidinic endonuclease 1; apurinic/a; Apurinic/apy; apurinic/apyrimidinic (abasic) endonuclease; apurinic/apyrimidinic endonuclease 1; deoxyribonuclease (apurinic or apyrimidinic); DNA repair nuclease/redox regulator APEX1; DNA-(apurinic or apyrimidinic site) endonuclease; DNA-(apurinic or apyrimidinic site) lyase; protein REF-1; redox factor 1; Redox factor-1; REF-1

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Gene Aliases: APE; APE1; APEN; APEX; APEX1; APX; HAP1; REF-1; REF1

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UniProt ID: (Human) P27695, (Mouse) P28352, (Rat) P43138

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Entrez Gene ID: (Human) 328, (Dog) 482558, (Mouse) 11792, (Rat) 79116

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Function(s)

DNA binding damaged DNA binding double-stranded telomeric DNA binding transcription coactivator activity transcription corepressor activity DNA-(apurinic or apyrimidinic site) lyase activity endonuclease activity endodeoxyribonuclease activity RNA-DNA hybrid ribonuclease activity phosphodiesterase I activity uracil DNA N-glycosylase activity protein binding phosphoric diester hydrolase activity double-stranded DNA exodeoxyribonuclease activity double-stranded DNA 3'-5' exodeoxyribonuclease activity 3'-5' exonuclease activity oxidoreductase activity site-specific endodeoxyribonuclease activity, specific for altered base chromatin DNA binding protein complex binding poly(A) RNA binding metal ion binding NF-kappaB binding RNA binding nuclease activity endoribonuclease activity exonuclease activity hydrolase activity lyase activity phosphoric ester hydrolase activity T/G mismatch-specific endonuclease activity retroviral 3' processing activity

Process(es)

DNA repair base-excision repair DNA recombination transcription, DNA-templated regulation of transcription, DNA-templated cellular response to DNA damage stimulus aging negative regulation of smooth muscle cell migration regulation of mRNA stability cell redox homeostasis positive regulation of DNA repair oxidation-reduction process cellular response to hydrogen peroxide DNA demethylation nucleic acid phosphodiester bond hydrolysis RNA phosphodiester bond hydrolysis, endonucleolytic positive regulation of G1/S transition of mitotic cell cycle regulation of nucleic acid-templated transcription telomere maintenance base-excision repair, base-free sugar-phosphate removal response to drug cellular response to cAMP cellular response to peptide hormone stimulus negative regulation of nucleic acid-templated transcription DNA catabolic process, endonucleolytic DNA catabolic process, exonucleolytic response to organonitrogen compound cellular response to organonitrogen compound

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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APE1 Monoclonal Antibody (13B 8E5C2)

APE1 Antibody (MA1-440) in ICC/IF

Product Details

MA1-440

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

ChIP assay (ChIP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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