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APEX1 Polyclonal Antibody

APEX1 Antibody in Immunocytochemistry (ICC/IF)

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APEX1 Antibody (10203-1-AP) in ICC/IF

Immunofluorescent analysis of MCF-7 cells, using APEX1 antibody 10203-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

APEX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

APEX1 Antibody in Immunoprecipitation (IP)

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Product Details

10203-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

APEX1 Fusion Protein Ag0251 (1-318 aa encoded by BC002338)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

10203-1-AP is a rabbit polyclonal antibody raised against full length APE1 of human origin.

Immunogen sequence: MPKRGKKGA VAEDGDELRT EPEAKKSKTA AKKNDKEAAG EGPALYEDPP DQKTSPSGKP ATLKICSWNV DGLRAWIKKK GLDWVKEEAP DILCLQETKC SENKLPAELQ ELPGLSHQYW SAPSDKEGYS GVGLLSRQCP LKVSYGIGEE EHDQEGRVIV AEFDSFVLVT AYVPNAGRGL VRLEYRQRWD EAFRKFLKGL ASRKPLVLCG DLNVAHEEID LRNPKGNKKN AGFTPQERQG FGELLQAVPL ADSFRHLYPN TPYAYTFWTY MMNARSKNVG WRLDYFLLSH SLLPALCDSK IRSKALGSDH CPITLYLAL (1-318 aa encoded by BC002338 )

Target Information

Mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is a multifunctional, bipartite enzyme that plays an important role in numerous, cellular functions. APE is responsible for repairing abasic sites in DNA and in regulating the redox state of other proteins that play roles in oxidative signaling, transcription factor regulation (Fos, Jun, NF-kB, Myb, HIF-1 alpha, CREB, Pax), cell cycle control (p53), and apoptosis. The most common form of DNA damage is the creation of abasic sites which are brought about through spontaneous loss or oxidative DNA damage, through chemically initiated hydrolysis (chemotherapy), ionizing radiation, UV irradiation, oxidizing agents, and removal of modified bases by DNA glycosylases. APE is differentially expressed during development and in different tissues. This protein has diverse subcellular localization patterns which support the possibility of its interaction with numerous, other cellular proteins in addition to DNA repair within the nucleus. Regulation of APE by phosphorylation is mediated, at least in part, by casein kinase II. Increases in APE message and protein levels are observed upon the reintroduction of oxygen to hypoxic cells, and in some malignant tissue relative to normal tissue. Decreases in APE expression have been associated with the induction of cellular apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Transient neurogenesis in ischemic cortex from Sox2⁺ astrocytes.

Neural regeneration research

Yang JL,Fan H,Fu FF,Guo BL,Huang Y,Sun L,Wang WT,Xing JL,Hu XT,Ding YQ,Zhang K,Hu YZ,Wang YZ

Sat Jul 01 00:00:00 EDT 2023

Apurinic/apyrimidinic endonuclease 1 regulates palmitic acid-mediated apoptosis in cardiomyocytes via endoplasmic reticulum stress.

Biochemical and biophysical research communications

Fan J,Liu M,Li X,Gao S,Wang Y,Li A,Chen L,Zhou D,Chen H,Xu Z,Wu Z,Wu K

Sun Apr 02 00:00:00 EDT 2023

Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair.

Science advances

Nguyen DD,Kim E,Le NT,Ding X,Jaiswal RK,Kostlan RJ,Nguyen TNT,Shiva O,Le MT,Chai W

Wed May 10 00:00:00 EDT 2023

NEDD4 attenuates oxidized low‑density lipoprotein‑induced inflammation and dysfunction in vascular endothelial cells via regulating APEX1 expression.

Experimental and therapeutic medicine

Xu H,Tan L,Qu Q,Zhang W

Wed Feb 01 00:00:00 EST 2023

APEX1 predicts poor prognosis of gallbladder cancer and affects biological properties of CD133⁺ GBC-SD cells via upregulating Jagged1.

Journal of Cancer

Wu Z,Liu Z,Sun Y,Yuan Y,Zou Q,Wen Y,Luo J,Liu R

Thu Jun 08 00:00:00 EDT 2023

APEX1 predicts poor prognosis of gallbladder cancer and affects biological properties of CD133⁺ GBC-SD cells via upregulating Jagged1.

Journal of Cancer

Wu Z,Liu Z,Sun Y,Yuan Y,Zou Q,Wen Y,Luo J,Liu R

Thu Jun 08 00:00:00 EDT 2023

Role of thioredoxin-interacting protein in mediating endothelial dysfunction in hypertension.

Genes & diseases

Wang R,Guo Y,Li L,Luo M,Peng L,Lv D,Cheng Z,Xue Q,Wang L,Huang J

Sun May 01 00:00:00 EDT 2022

E2F1/CKS2/PTEN signaling axis regulates malignant phenotypes in pediatric retinoblastoma.

Cell death & disease

Chen M,Zhao Z,Wu L,Huang J,Yu P,Qian J,Ni T,Zhao C

Mon Sep 12 00:00:00 EDT 2022

Recruitment of DNA to tumor-derived microvesicles.

Cell reports

Clancy JW,Sheehan CS,Boomgarden AC,D'Souza-Schorey C

Tue Mar 01 00:00:00 EST 2022

LINC00470 accelerates the proliferation and metastasis of melanoma through promoting APEX1 expression.

Cell death & disease

Huang T,Wang YJ,Huang MT,Guo Y,Yang LC,Liu XJ,Tan WY,Long JH

Mon Apr 19 00:00:00 EDT 2021

LINC00470 accelerates the proliferation and metastasis of melanoma through promoting APEX1 expression.

Cell death & disease

Huang T,Wang YJ,Huang MT,Guo Y,Yang LC,Liu XJ,Tan WY,Long JH

Mon Apr 19 00:00:00 EDT 2021

Small-molecule inhibition of APE1 induces apoptosis, pyroptosis, and necroptosis in non-small cell lung cancer.

Cell death & disease

Long K,Gu L,Li L,Zhang Z,Li E,Zhang Y,He L,Pan F,Guo Z,Hu Z

Tue May 18 00:00:00 EDT 2021

miR-1293, a Candidate for miRNA-Based Cancer Therapeutics, Simultaneously Targets BRD4 and the DNA Repair Pathway.

Molecular therapy : the journal of the American Society of Gene Therapy

Takagawa Y,Gen Y,Muramatsu T,Tanimoto K,Inoue J,Harada H,Inazawa J

Wed Jun 03 00:00:00 EDT 2020

miR-1293, a Candidate for miRNA-Based Cancer Therapeutics, Simultaneously Targets BRD4 and the DNA Repair Pathway.

Molecular therapy : the journal of the American Society of Gene Therapy

Takagawa Y,Gen Y,Muramatsu T,Tanimoto K,Inoue J,Harada H,Inazawa J

Wed Jun 03 00:00:00 EDT 2020

MicroRNA-765 sensitizes osteosarcoma cells to cisplatin via downregulating APE1 expression.

OncoTargets and therapy

Liang W,Li C,Li M,Wang D,Zhong Z

Sun Apr 10 00:00:00 EDT 2022

An integrated approach to identify critical transcription factors in the protection against hydrogen peroxide-induced oxidative stress by Danhong injection.

Free radical biology & medicine

Zhang J,Guo F,Wei J,Xian M,Tang S,Zhao Y,Liu M,Song L,Geng Y,Yang H,Ding C,Huang L

Wed Nov 01 00:00:00 EDT 2017

An integrated approach to identify critical transcription factors in the protection against hydrogen peroxide-induced oxidative stress by Danhong injection.

Free radical biology & medicine

Zhang J,Guo F,Wei J,Xian M,Tang S,Zhao Y,Liu M,Song L,Geng Y,Yang H,Ding C,Huang L

Wed Nov 01 00:00:00 EDT 2017

Screening and identification of critical transcription factors involved in the protection of cardiomyocytes against hydrogen peroxide-induced damage by Yixin-shu.

Scientific reports

Zhang J,Geng Y,Guo F,Zhang F,Liu M,Song L,Ma Y,Li D,Zhang Y,Xu H,Yang H

Tue Oct 24 00:00:00 EDT 2017

Screening and identification of critical transcription factors involved in the protection of cardiomyocytes against hydrogen peroxide-induced damage by Yixin-shu.

Scientific reports

Zhang J,Geng Y,Guo F,Zhang F,Liu M,Song L,Ma Y,Li D,Zhang Y,Xu H,Yang H

Tue Oct 24 00:00:00 EDT 2017

Screening and identification of critical transcription factors involved in the protection of cardiomyocytes against hydrogen peroxide-induced damage by Yixin-shu.

Scientific reports

Zhang J,Geng Y,Guo F,Zhang F,Liu M,Song L,Ma Y,Li D,Zhang Y,Xu H,Yang H

Tue Oct 24 00:00:00 EDT 2017

Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.

Toxicology and applied pharmacology

Dong H,Shi Q,Song X,Fu J,Hu L,Xu D,Su C,Xia X,Song E,Song Y

Wed Jul 01 00:00:00 EDT 2015

Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.

Toxicology and applied pharmacology

Dong H,Shi Q,Song X,Fu J,Hu L,Xu D,Su C,Xia X,Song E,Song Y

Wed Jul 01 00:00:00 EDT 2015

Suppression of oxidative phosphorylation in mouse embryonic fibroblast cells deficient in apurinic/apyrimidinic endonuclease.

DNA repair

Suganya R,Chakraborty A,Miriyala S,Hazra TK,Izumi T

Sun Mar 01 00:00:00 EST 2015

Suppression of oxidative phosphorylation in mouse embryonic fibroblast cells deficient in apurinic/apyrimidinic endonuclease.

DNA repair

Suganya R,Chakraborty A,Miriyala S,Hazra TK,Izumi T

Sun Mar 01 00:00:00 EST 2015

miR-130a can predict response to temozolomide in patients with glioblastoma multiforme, independently of O6-methylguanine-DNA methyltransferase.

Journal of translational medicine

Chen H,Li X,Li W,Zheng H

Sat Feb 21 00:00:00 EST 2015

miR-130a can predict response to temozolomide in patients with glioblastoma multiforme, independently of O6-methylguanine-DNA methyltransferase.

Journal of translational medicine

Chen H,Li X,Li W,Zheng H

Sat Feb 21 00:00:00 EST 2015

Apurinic/apyrimidinic endonuclease 1 is the essential nuclease during immunoglobulin class switch recombination.

Molecular and cellular biology

Masani S,Han L,Yu K

Mon Apr 01 00:00:00 EDT 2013

Apurinic/apyrimidinic endonuclease 1 is the essential nuclease during immunoglobulin class switch recombination.

Molecular and cellular biology

Masani S,Han L,Yu K

Mon Apr 01 00:00:00 EDT 2013

Induction of base excision repair enzymes NTH1 and APE1 in rat spleen following aniline exposure.

Toxicology and applied pharmacology

Ma H,Wang J,Abdel-Rahman SZ,Boor PJ,Khan MF

Fri Mar 15 00:00:00 EDT 2013

Induction of base excision repair enzymes NTH1 and APE1 in rat spleen following aniline exposure.

Toxicology and applied pharmacology

Ma H,Wang J,Abdel-Rahman SZ,Boor PJ,Khan MF

Fri Mar 15 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: AP endonuclease 1; AP endonuclease class I; AP lyase; APEN; APEX nuclease; APEX nuclease (multifunctional DNA repair enzyme) 1; APEX nuclease 1; Apurinic-apyrimidinic endonuclease 1; apurinic/a; Apurinic/apy; apurinic/apyrimidinic (abasic) endonuclease; apurinic/apyrimidinic endonuclease 1; deoxyribonuclease (apurinic or apyrimidinic); DNA repair nuclease/redox regulator APEX1; DNA-(apurinic or apyrimidinic site) endonuclease; DNA-(apurinic or apyrimidinic site) lyase; protein REF-1; redox factor 1; Redox factor-1; REF-1

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Gene Aliases: APE; APE1; APEN; APEX; APEX1; APX; HAP1; REF-1; REF1

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UniProt ID: (Human) P27695, (Mouse) P28352, (Rat) P43138

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Entrez Gene ID: (Human) 328, (Mouse) 11792, (Rat) 79116

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Function(s)

DNA binding damaged DNA binding double-stranded telomeric DNA binding transcription coactivator activity transcription corepressor activity DNA-(apurinic or apyrimidinic site) lyase activity endonuclease activity endodeoxyribonuclease activity RNA-DNA hybrid ribonuclease activity phosphodiesterase I activity uracil DNA N-glycosylase activity protein binding phosphoric diester hydrolase activity double-stranded DNA exodeoxyribonuclease activity double-stranded DNA 3'-5' exodeoxyribonuclease activity 3'-5' exonuclease activity oxidoreductase activity site-specific endodeoxyribonuclease activity, specific for altered base chromatin DNA binding protein complex binding poly(A) RNA binding metal ion binding NF-kappaB binding RNA binding nuclease activity endoribonuclease activity exonuclease activity hydrolase activity lyase activity phosphoric ester hydrolase activity T/G mismatch-specific endonuclease activity retroviral 3' processing activity

Process(es)

DNA repair base-excision repair DNA recombination transcription, DNA-templated regulation of transcription, DNA-templated cellular response to DNA damage stimulus aging negative regulation of smooth muscle cell migration regulation of mRNA stability cell redox homeostasis positive regulation of DNA repair oxidation-reduction process cellular response to hydrogen peroxide DNA demethylation nucleic acid phosphodiester bond hydrolysis RNA phosphodiester bond hydrolysis, endonucleolytic positive regulation of G1/S transition of mitotic cell cycle regulation of nucleic acid-templated transcription telomere maintenance base-excision repair, base-free sugar-phosphate removal response to drug cellular response to cAMP cellular response to peptide hormone stimulus negative regulation of nucleic acid-templated transcription DNA catabolic process, endonucleolytic DNA catabolic process, exonucleolytic response to organonitrogen compound cellular response to organonitrogen compound

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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APEX1 Polyclonal Antibody

APEX1 Antibody (10203-1-AP) in ICC/IF

Product Details

10203-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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