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APIP Antibody (PA5-29269) in IHC (P)
APIP Polyclonal Antibody detects APAF1-interacting protein protein at cytoplasm on human placenta by immunohistochemical analysis. Sample: Paraffin-embedded placenta. APIP Polyclonal Antibody (Product # PA5-29269) dilution: 1:100. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-29269
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full length human APIP Recombinant protein.
Conjugate
Form
Liquid
Concentration
1.01 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2546745
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
Predicted reactivity: Mouse (94%), Zebrafish (82%), Xenopus laevis (82%), Chicken (87%), Bovine (91%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf 1 (for apoptosis protease-activating factor 1). Apaf 1 binds to cytochrome c (Apaf 2) and Caspase-9 (Apaf 3), which leads to Caspase-9 activation. Activated Caspase-9 in turn cleaves and activates Caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Recently, Cho et al (4) have identified a new Apaf 1 Interactiong Protein (APIP) also known as CG129 and MMRP19, as a negative regulator of ischemic injury. APIP competes with Caspase-9 binding site of Apaf 1. APIP is predicted to code for a 204 amino acid. An isoform of APIP, APIP2 encodes a 242 amino acid protein, which is an alternative splicing variant differing in its N-terminus from APIP. APIP transcript is ubiquitously expressed in most adult tissue with high expression in skeletal muscle, heart, and kidney.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APAF1-interacting protein; APIP; CG129; dJ179L10.2; hAPIP; Methylthioribulose-1-phosphate dehydratase; MTRu-1-P dehydratase; probable methylthioribulose-1-phosphate dehydratase
Gene Aliases: APIP; APIP2; CGI-29; CGI29; hAPIP; MMRP19
UniProt ID: (Human) Q96GX9
Entrez Gene ID: (Human) 51074
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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