Proteintech

APOB Polyclonal Antibody

APOB Antibody in Immunocytochemistry (ICC/IF)

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APOB Antibody (20578-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 20578-1-AP (APOB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

APOB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

20578-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 25 publications 25 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.18 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody recognizes both of ApoB-48 and ApoB-100.

Target Information

Apolipoprotein B (apo B) in human plasma is a major protein of low density lipoproteins (LDL) with a molecular mass of approximately 260-500 kDa. Apolipoprotein B binds to specific receptors on cell membranes and is involved in removal of LDL and very low density lipoprotein (VLDL) cholesterol from circulation. Low-density lipoprotein (LDL) is the carrier protein for cholesterol in the blood. LDL binds to its receptor on the capillary walls and thereby mediates the uptake and clearance of cholesterol from the circulation. In atherosclerotic lesions oxidatively modified LDL is found and oxidized LDL is specifically recognized and ingested by macrophages via scavenger receptor A and CD36. Oxidized LDL may be a marker of atherosclerosis but the precise changes in oxidized LDL are not well described. MDA-oxidized LDL appear to be different from LDL oxidized by other means. Apolipoprotein B is mostly synthesized in the liver, and is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800mg/L. Apolipoprotein B occurs in plasma as two main isoforms, apoB-48 and apoB-100. The intestinal and the hepatic forms of Apolipoprotein B are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in the Apolipoprotein B gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective Apolipoprotein B, diseases affecting plasma cholesterol and Apolipoprotein B levels.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CM3-SII polysaccharide obtained from Cordyceps militaris ameliorates hyperlipidemia in heterozygous LDLR-deficient hamsters by modulating gut microbiota and NPC1L1 and PPARα levels.

International journal of biological macromolecules

Yu WQ,Wang XL,Ji HH,Miao M,Zhang BH,Li H,Zhang ZY,Ji CF,Guo SD

Thu Jun 01 00:00:00 EDT 2023

Comparison of proteomic landscape of extracellular vesicles in pleural effusions isolated by three strategies.

Frontiers in bioengineering and biotechnology

Yao X,Liao B,Chen F,Liu L,Wu K,Hao Y,Li Y,Wang Y,Fan R,Yin J,Liu L,Guo Y

Tue May 02 00:00:00 EDT 2023

Mechanical injury accentuates lipid deposition in ApoE^-/- mice and advance aortic valve stenosis: A novel modified aortic valve stenosis model.

Frontiers in cardiovascular medicine

Wen D,Hu L,Shan J,Zhang H,Hu L,Yuan A,Pu J,Xue S

Fri Feb 24 00:00:00 EST 2023

Fenofibrate enhances lipid deposition via modulating PPARγ, SREBP-1c, and gut microbiota in ob/ob mice fed a high-fat diet.

Frontiers in nutrition

Zhang Y,Jia XB,Liu YC,Yu WQ,Si YH,Guo SD

Fri Sep 30 00:00:00 EDT 2022

Liver RBFOX2 regulates cholesterol homeostasis via Scarb1 alternative splicing in mice.

Nature metabolism

Paterson HAB,Yu S,Artigas N,Prado MA,Haberman N,Wang YF,Jobbins AM,Pahita E,Mokochinski J,Hall Z,Guerin M,Paulo JA,Ng SS,Villarroya F,Rashid ST,Le Goff W,Lenhard B,Cebola I,Finley D,Gygi SP,Sibley CR,Vernia S

Thu Dec 01 00:00:00 EST 2022

Characteristics of Exosomes and the Vascular Landscape Regulate Exosome Sequestration by Peripheral Tissues and Brain.

International journal of molecular sciences

Banks WA,Sharma P,Hansen KM,Ludwig N,Whiteside TL

Wed Oct 19 00:00:00 EDT 2022

Enterically derived high-density lipoprotein restrains liver injury through the portal vein.

Science (New York, N.Y.)

Han YH,Onufer EJ,Huang LH,Sprung RW,Davidson WS,Czepielewski RS,Wohltmann M,Sorci-Thomas MG,Warner BW,Randolph GJ

Fri Jul 23 00:00:00 EDT 2021

The Cordyceps militaris-Derived Polysaccharide CM1 Alleviates Atherosclerosis in LDLR^(-/-) Mice by Improving Hyperlipidemia.

Frontiers in molecular biosciences

Yin F,Lin P,Yu WQ,Shen N,Li Y,Guo SD

Fri Dec 31 00:00:00 EST 2021

Polysaccharide CM1 from Cordyceps militaris hinders adipocyte differentiation and alleviates hyperlipidemia in LDLR^(+/-) hamsters.

Lipids in health and disease

Yu WQ,Yin F,Shen N,Lin P,Xia B,Li YJ,Guo SD

Mon Dec 13 00:00:00 EST 2021

Quantitative proteomics of plasma and liver reveals the mechanism of turmeric in preventing hyperlipidemia in mice.

Food & function

Wang M,Wang R,Li L,Yan Y,Jia S,Jiang H,Du Z

Mon Nov 01 00:00:00 EDT 2021

MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.

Biomaterials

Song M,Zhang S,Tao Z,Li J,Shi Y,Xiong Y,Zhang W,Liu C,Chen S

Mon Nov 01 00:00:00 EDT 2021

GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity.

Nature communications

Peng Y,Zeng Q,Wan L,Ma E,Li H,Yang X,Zhang Y,Huang L,Lin H,Feng J,Xu Y,Li J,Liu M,Liu J,Lin C,Sun Z,Cheng G,Zhang X,Liu J,Li D,Wei M,Mo Y,Mu X,Deng X,Zhang D,Dong S,Huang H,Fang Y,Gao Q,Yang X,Wu F,Zhong H,Wei C

Wed Dec 01 00:00:00 EST 2021

Deficiency of intestinal Bmal1 prevents obesity induced by high-fat feeding.

Nature communications

Yu F,Wang Z,Zhang T,Chen X,Xu H,Wang F,Guo L,Chen M,Liu K,Wu B

Tue Sep 07 00:00:00 EDT 2021

MD2 blockade prevents modified LDL-induced retinal injury in diabetes by suppressing NADPH oxidase-4 interaction with Toll-like receptor-4.

Experimental & molecular medicine

Chen H,Yan T,Song Z,Ying S,Wu B,Ju X,Yang X,Qu J,Wu W,Zhang Z,Wang Y

Thu Apr 01 00:00:00 EDT 2021

The miR-378c-Samd1 circuit promotes phenotypic modulation of vascular smooth muscle cells and foam cells formation in atherosclerosis lesions.

Scientific reports

Tian S,Cao Y,Wang J,Bi Y,Zhong J,Meng X,Sun W,Yang R,Gan L,Wang X,Li H,Wang R

Tue May 18 00:00:00 EDT 2021

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Cell metabolism

Huang D,Xu B,Liu L,Wu L,Zhu Y,Ghanbarpour A,Wang Y,Chen FJ,Lyu J,Hu Y,Kang Y,Zhou W,Wang X,Ding W,Li X,Jiang Z,Chen J,Zhang X,Zhou H,Li JZ,Guo C,Zheng W,Zhang X,Li P,Melia T,Reinisch K,Chen XW

Tue Aug 03 00:00:00 EDT 2021

Celastrol inhibits intestinal lipid absorption by reprofiling the gut microbiota to attenuate high-fat diet-induced obesity.

iScience

Hua H,Zhang Y,Zhao F,Chen K,Wu T,Liu Q,Huang S,Zhang A,Jia Z

Fri Feb 19 00:00:00 EST 2021

Downregulation of GNAI3 Promotes the Pathogenesis of Methionine/Choline-Deficient Diet-Induced Nonalcoholic Fatty Liver Disease.

Gut and liver

Zhu H,Ge K,Lu J,Jia C

Wed Jul 15 00:00:00 EDT 2020

Small sequence variations between two mammalian paralogs of the small GTPase SAR1 underlie functional differences in coat protein complex II assembly.

The Journal of biological chemistry

Melville DB,Studer S,Schekman R

Fri Jun 19 00:00:00 EDT 2020

Effect and mechanism of ginsenoside Rg1-regulating hepatic steatosis in HepG2 cells induced by free fatty acid.

Bioscience, biotechnology, and biochemistry

Gao Y,Zhang S,Li J,Zhao J,Xiao Q,Zhu Y,Zhang J,Huang W

Sun Nov 01 00:00:00 EDT 2020

Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.

The Journal of experimental medicine

Wang Y,Song W,Wang J,Wang T,Xiong X,Qi Z,Fu W,Yang X,Chen YG

Mon Feb 03 00:00:00 EST 2020

Macrophage-derived myeloid differentiation protein 2 plays an essential role in ox-LDL-induced inflammation and atherosclerosis.

EBioMedicine

Chen T,Huang W,Qian J,Luo W,Shan P,Cai Y,Lin K,Wu G,Liang G

Sun Mar 01 00:00:00 EST 2020

Macrophage-derived myeloid differentiation protein 2 plays an essential role in ox-LDL-induced inflammation and atherosclerosis.

EBioMedicine

Chen T,Huang W,Qian J,Luo W,Shan P,Cai Y,Lin K,Wu G,Liang G

Sun Mar 01 00:00:00 EST 2020

Comprehensive lipidomics in apoM^-/- mice reveals an overall state of metabolic distress and attenuated hepatic lipid secretion into the circulation.

Journal of genetics and genomics = Yi chuan xue bao

Shi Y,Lam SM,Liu H,Luo G,Zhang J,Yao S,Li J,Zheng L,Xu N,Zhang X,Shui G

Sun Sep 20 00:00:00 EDT 2020

Hepatic HuR modulates lipid homeostasis in response to high-fat diet.

Nature communications

Zhang Z,Zong C,Jiang M,Hu H,Cheng X,Ni J,Yi X,Jiang B,Tian F,Chang MW,Su W,Zhu L,Li J,Xiang X,Miao C,Gorospe M,de Cabo R,Dou Y,Ju Z,Yang J,Jiang C,Yang Z,Wang W

Tue Jun 16 00:00:00 EDT 2020

Extracellular vesicle-mediated delivery of miR-101 inhibits lung metastasis in osteosarcoma.

Theranostics

Zhang K,Dong C,Chen M,Yang T,Wang X,Gao Y,Wang L,Wen Y,Chen G,Wang X,Yu X,Zhang Y,Wang P,Shang M,Han K,Zhou Y

Fri Apr 02 00:00:00 EDT 2021

AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.

Cell metabolism

Luo H,Jiang M,Lian G,Liu Q,Shi M,Li TY,Song L,Ye J,He Y,Yao L,Zhang C,Lin ZZ,Zhang CS,Zhao TJ,Jia WP,Li P,Lin SY,Lin SC

Tue Apr 03 00:00:00 EDT 2018

AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.

Cell metabolism

Luo H,Jiang M,Lian G,Liu Q,Shi M,Li TY,Song L,Ye J,He Y,Yao L,Zhang C,Lin ZZ,Zhang CS,Zhao TJ,Jia WP,Li P,Lin SY,Lin SC

Tue Apr 03 00:00:00 EDT 2018

Hepatic loss of Lissencephaly 1 (Lis1) induces fatty liver and accelerates liver tumorigenesis in mice.

The Journal of biological chemistry

Li X,Liu L,Li R,Wu A,Lu J,Wu Q,Jia J,Zhao M,Song H

Fri Apr 06 00:00:00 EDT 2018

Hepatic loss of Lissencephaly 1 (Lis1) induces fatty liver and accelerates liver tumorigenesis in mice.

The Journal of biological chemistry

Li X,Liu L,Li R,Wu A,Lu J,Wu Q,Jia J,Zhao M,Song H

Fri Apr 06 00:00:00 EDT 2018

Rab8a Deficiency in Skeletal Muscle Causes Hyperlipidemia and Hepatosteatosis by Impairing Muscle Lipid Uptake and Storage.

Diabetes

Chen Q,Rong P,Xu D,Zhu S,Chen L,Xie B,Du Q,Quan C,Sheng Y,Zhao TJ,Li P,Wang HY,Chen S

Fri Sep 01 00:00:00 EDT 2017

Rab8a Deficiency in Skeletal Muscle Causes Hyperlipidemia and Hepatosteatosis by Impairing Muscle Lipid Uptake and Storage.

Diabetes

Chen Q,Rong P,Xu D,Zhu S,Chen L,Xie B,Du Q,Quan C,Sheng Y,Zhao TJ,Li P,Wang HY,Chen S

Fri Sep 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: Apo B-100; Apo B100; apo-B; ApoB 100; ApoB 48; apolipo b; apolipoprotein B (including Ag(x) antigen); Apolipoprotein B 100; Apolipoprotein B 48; Apolipoprotein B-100; Apolipoprotein B100; apolipoprotein B46; apolipoprotein B47; apolipoprotein B48; apolipoprotein B49; mCG_129875; MGC176318

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Gene Aliases: AI315052; Apo B-100; APOB; apoB-100; apoB-48; FLDB; LDLCQ4

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UniProt ID: (Human) P04114, (Mouse) E9Q414

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Entrez Gene ID: (Human) 338, (Mouse) 238055

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Function(s)

protein binding phospholipid binding heparin binding cholesterol transporter activity lipase binding low-density lipoprotein particle receptor binding lipid transporter activity lipid binding apolipoprotein

Process(es)

retinoid metabolic process in utero embryonic development triglyceride mobilization receptor-mediated endocytosis spermatogenesis nervous system development cholesterol metabolic process fertilization response to virus response to carbohydrate post-embryonic development response to selenium ion positive regulation of gene expression positive regulation of macrophage derived foam cell differentiation positive regulation of lipid storage positive regulation of cholesterol storage triglyceride catabolic process cholesterol transport sperm motility response to lipopolysaccharide cholesterol efflux low-density lipoprotein particle remodeling very-low-density lipoprotein particle assembly low-density lipoprotein particle clearance lipoprotein metabolic process lipoprotein biosynthetic process lipoprotein catabolic process cholesterol homeostasis lipoprotein transport cellular protein catabolic process regulation of cholesterol biosynthetic process artery morphogenesis leukocyte migration cellular response to tumor necrosis factor cellular response to prostaglandin stimulus lipid metabolic process transport lipid transport steroid metabolic process lipid catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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APOB Polyclonal Antibody

APOB Antibody (20578-1-AP) in ICC/IF

Product Details

20578-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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