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APOE Antibody (MA5-50301) in WB
Western Blot analysis of APOE using APOE Monoclonal Antibody (Product # MA5-50301) in Human plasma (30 μg) at a dilution of 1:25,000. Extracts were separated by 10% SDS PAGE. An HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Product Details
MA5-50301
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
GT1627
Immunogen
Recombinant protein encompassing a sequence within the center region of human Apolipoprotein E. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 20% glycerol
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3093485
Product Specific Information
This product can be used in Sandwich ELISA to detect APOE using MA5-50301 as a capture antibody and PA5-27088 as the detection antibody.
Target Information
Apo E (Apolipoprotein E) plays an important role in the metabolism of lipids in the plasma, and is also is a constituent of various plasma lipoprotein-lipid particles. The protein is synthesized in liver, brain, spleen, and the kidney, and concentrations are high in interstitial fluids, where it plays a role in distributing cholesterol from cells where cholesterol is in excess to cells which require cholesterol. Apo E also plays an important role in the formation of very low density lipoprotein and chylomicrons. The Apo E gene is located on chromosome 19 at position 19q13. 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Apo-E; ApoE4; Apolipoprotein; Apolipoprotein E; apolipoprotein E3; apolipoprotein E4; Apoprotein; B2G1; BG; MGC1571
Gene Aliases: AD2; APO-E; APOE; APOEA; LDLCQ5; LPG
UniProt ID: (Human) P02649, (Rat) P02650
Entrez Gene ID: (Human) 348, (Rat) 25728
beta-amyloid binding
lipid transporter activity
protein binding
phospholipid binding
heparin binding
lipid binding
cholesterol binding
antioxidant activity
cholesterol transporter activity
identical protein binding
protein homodimerization activity
metal chelating activity
tau protein binding
low-density lipoprotein particle receptor binding
phosphatidylcholine-sterol O-acyltransferase activator activity
very-low-density lipoprotein particle receptor binding
lipoprotein particle binding
receptor binding
hydroxyapatite binding
apolipoprotein
response to reactive oxygen species
retinoid metabolic process
negative regulation of endothelial cell proliferation
response to dietary excess
triglyceride metabolic process
cholesterol catabolic process
cellular calcium ion homeostasis
receptor-mediated endocytosis
cytoskeleton organization
G-protein coupled receptor signaling pathway
nitric oxide mediated signal transduction
synaptic transmission, cholinergic
cholesterol metabolic process
regulation of gene expression
negative regulation of platelet activation
positive regulation of cholesterol esterification
positive regulation of cholesterol efflux
long-chain fatty acid transport
protein import
virion assembly
triglyceride catabolic process
cGMP-mediated signaling
negative regulation of blood coagulation
regulation of axon extension
positive regulation of cGMP biosynthetic process
neuron projection regeneration
regulation of Cdc42 protein signal transduction
positive regulation of low-density lipoprotein particle receptor catabolic process
cholesterol efflux
phospholipid efflux
very-low-density lipoprotein particle remodeling
low-density lipoprotein particle remodeling
high-density lipoprotein particle remodeling
high-density lipoprotein particle assembly
chylomicron remnant clearance
high-density lipoprotein particle clearance
very-low-density lipoprotein particle clearance
lipoprotein metabolic process
lipoprotein biosynthetic process
lipoprotein catabolic process
vasodilation
cholesterol homeostasis
negative regulation of MAP kinase activity
negative regulation of neuron apoptotic process
negative regulation of blood vessel endothelial cell migration
reverse cholesterol transport
positive regulation by host of viral process
negative regulation of cholesterol biosynthetic process
positive regulation of lipid biosynthetic process
intracellular transport
regulation of neuronal synaptic plasticity
artery morphogenesis
negative regulation of inflammatory response
positive regulation of nitric-oxide synthase activity
positive regulation of membrane protein ectodomain proteolysis
maintenance of location in cell
fatty acid homeostasis
positive regulation of dendritic spine development
negative regulation of canonical Wnt signaling pathway
AMPA glutamate receptor clustering
NMDA glutamate receptor clustering
cellular oxidant detoxification
regulation of beta-amyloid clearance
negative regulation of neuron death
positive regulation of postsynaptic membrane organization
negative regulation of presynaptic membrane organization
negative regulation of beta-amyloid formation
positive regulation of dendritic spine maintenance
positive regulation of phospholipid efflux
positive regulation of lipid transport across blood brain barrier
lipid metabolic process
cholesterol biosynthetic process
lipid transport
response to oxidative stress
aging
protein localization
peripheral nervous system axon regeneration
axon regeneration
regulation of cholesterol transport
response to retinoic acid
response to insulin
positive regulation of catalytic activity
response to ethanol
positive regulation of axon extension
oligodendrocyte differentiation
negative regulation of lipid biosynthetic process
lipid homeostasis
negative regulation of dendritic spine development
cellular response to interleukin-1
cellular response to growth factor stimulus
cellular response to cholesterol
cardiovascular system development
negative regulation of cholesterol efflux
regulation of neuron death
positive regulation of neuron death
negative regulation of postsynaptic membrane organization
positive regulation of presynaptic membrane organization
positive regulation of beta-amyloid formation
regulation of tau-protein kinase activity
negative regulation of dendritic spine maintenance
positive regulation of neurofibrillary tangle assembly
negative regulation of phospholipid efflux
negative regulation of lipid transport across blood brain barrier
Performance Guarantee
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