Proteintech

APOL1 Polyclonal Antibody

APOL1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 26

APOL1 Antibody (11486-2-AP) in ICC/IF

Immunofluorescent analysis of ( ) fixed HepG2 cells using 11486-2-AP (APOL1 antibody) at dilution of 1:25.

APOL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

APOL1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11486-2-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 4 publications 4 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

APOL1 Fusion Protein Ag2016 (1-238 aa encoded by BC017331)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.44 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody recognizes the endogenous APOL1 of 39-45 kDa in blood lysate.

Immunogen sequence: MEGAALLRV SVLCIWMSAL FLGVGVRAEE AGARVQQNVP SGTDTGDPQS KPLGDWAAGT MDPESSIFIE DAIKYFKEKV STQNLLLLLT DNEAWNGFVA AAELPRNEAD ELRKALDNLA RQMIMKDKNW HDKGQQYRNW FLKEFPRLKS ELEDNIRRLR ALADGVQKVH KGTTIANVVS GSLSISSGIL TLVGMGLAPF TEGGSLVLLE PGMELGITAA LTGITSSTMD YGKKWWTQA (1-238 aa encoded by BC017331 )

Target Information

APOL1 is a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Apolipoprotein L1 (APOL1) cation current in HEK-293 cells and in human podocytes.

Pflugers Archiv : European journal of physiology

Vandorpe DH,Heneghan JF,Waitzman JS,McCarthy GM,Blasio A,Magraner JM,Donovan OG,Schaller LB,Shah SS,Subramanian B,Riella CV,Friedman DJ,Pollak MR,Alper SL

Wed Mar 01 00:00:00 EST 2023

HDL Isolated by Immunoaffinity, Ultracentrifugation, or Precipitation is Compositionally and Functionally Distinct.

Journal of lipid research

Holzer M,Ljubojevic-Holzer S,Souza Junior DR,Stadler JT,Rani A,Scharnagl H,Ronsein GE,Marsche G

Thu Dec 01 00:00:00 EST 2022

Genome-Wide Identification of Autophagy Prognostic Signature in Pancreatic Cancer.

Dose-response : a publication of International Hormesis Society

Yu J,Lang Q,Zhong C,Wang S,Tian Y

Sun Apr 24 00:00:00 EDT 2022

Structures of the ApoL1 and ApoL2 N-terminal domains reveal a non-classical four-helix bundle motif.

Communications biology

Ultsch M,Holliday MJ,Gerhardy S,Moran P,Scales SJ,Gupta N,Oltrabella F,Chiu C,Fairbrother W,Eigenbrot C,Kirchhofer D

Tue Jul 27 00:00:00 EDT 2021

APOL1 risk variants affect podocyte lipid homeostasis and energy production in focal segmental glomerulosclerosis.

Human molecular genetics

Ge M,Molina J,Ducasa GM,Mallela SK,Varona Santos J,Mitrofanova A,Kim JJ,Liu X,Sloan A,Mendez AJ,Banerjee S,Liu S,Szeto HH,Shin MK,Hoek M,Kopp JB,Fontanesi F,Merscher S,Fornoni A

Mon Apr 26 00:00:00 EDT 2021

Interplay of Trypanosome Lytic Factor and innate immune cells in the resolution of cutaneous Leishmania infection.

PLoS pathogens

Pant J,Samanovic M,Nelson MT,Keceli MK,Verdi J,Beverley SM,Raper J

Wed Sep 01 00:00:00 EDT 2021

Vacuolar ATPase depletion contributes to dysregulation of endocytosis in bloodstream forms of Trypanosoma brucei.

Parasites & vectors

Xu ZS,Li FJ,Hide G,Lun ZR,Lai DH

Sat Apr 25 00:00:00 EDT 2020

Apolipoprotein L1-Specific Antibodies Detect Endogenous APOL1 inside the Endoplasmic Reticulum and on the Plasma Membrane of Podocytes.

Journal of the American Society of Nephrology : JASN

Scales SJ,Gupta N,De Mazière AM,Posthuma G,Chiu CP,Pierce AA,Hötzel K,Tao J,Foreman O,Koukos G,Oltrabella F,Klumperman J,Lin W,Peterson AS

Tue Sep 01 00:00:00 EDT 2020

Apolipoprotein L1-Specific Antibodies Detect Endogenous APOL1 inside the Endoplasmic Reticulum and on the Plasma Membrane of Podocytes.

Journal of the American Society of Nephrology : JASN

Scales SJ,Gupta N,De Mazière AM,Posthuma G,Chiu CP,Pierce AA,Hötzel K,Tao J,Foreman O,Koukos G,Oltrabella F,Klumperman J,Lin W,Peterson AS

Tue Sep 01 00:00:00 EDT 2020

Domain-Specific Antibodies Reveal Differences in the Membrane Topologies of Apolipoprotein L1 in Serum and Podocytes.

Journal of the American Society of Nephrology : JASN

Gupta N,Wang X,Wen X,Moran P,Paluch M,Hass PE,Heidersbach A,Haley B,Kirchhofer D,Brezski RJ,Peterson AS,Scales SJ

Tue Sep 01 00:00:00 EDT 2020

Apolipoprotein L-1 renal risk variants form active channels at the plasma membrane driving cytotoxicity.

eLife

Giovinazzo JA,Thomson RP,Khalizova N,Zager PJ,Malani N,Rodriguez-Boulan E,Raper J,Schreiner R

Tue May 19 00:00:00 EDT 2020

MiR193a Modulation and Podocyte Phenotype.

Cells

Jha A,Saha S,Ayasolla K,Vashistha H,Malhotra A,Skorecki K,Singhal PC

Fri Apr 17 00:00:00 EDT 2020

All You Ever Wanted to Know About APOL1 and TLFs and Did Not Dare Ask.

Methods in molecular biology (Clifton, N.J.)

Verdi J,Schaub C,Thomson R,Raper J

Mon Feb 01 00:00:00 EST 2021

Antisense oligonucleotide treatment ameliorates IFN-γ-induced proteinuria in APOL1-transgenic mice.

JCI insight

Aghajan M,Booten SL,Althage M,Hart CE,Ericsson A,Maxvall I,Ochaba J,Menschik-Lundin A,Hartleib J,Kuntz S,Gattis D,Ahlström C,Watt AT,Engelhardt JA,Monia BP,Magnone MC,Guo S

Thu Jun 20 00:00:00 EDT 2019

Basal expression of interferon regulatory factor 1 drives intrinsic hepatocyte resistance to multiple RNA viruses.

Nature microbiology

Yamane D,Feng H,Rivera-Serrano EE,Selitsky SR,Hirai-Yuki A,Das A,McKnight KL,Misumi I,Hensley L,Lovell W,González-López O,Suzuki R,Matsuda M,Nakanishi H,Ohto-Nakanishi T,Hishiki T,Wauthier E,Oikawa T,Morita K,Reid LM,Sethupathy P,Kohara M,Whitmire JK,Lemon SM

Mon Jul 01 00:00:00 EDT 2019

The effect of normal human serum on the mouse trypanosome Trypanosoma musculi in vitro and in vivo.

Experimental parasitology

Zhang X,Hong XK,Li SJ,Lai DH,Hide G,Lun ZR,Wen YZ

Mon Jan 01 00:00:00 EST 2018

The effect of normal human serum on the mouse trypanosome Trypanosoma musculi in vitro and in vivo.

Experimental parasitology

Zhang X,Hong XK,Li SJ,Lai DH,Hide G,Lun ZR,Wen YZ

Mon Jan 01 00:00:00 EST 2018

A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.

British journal of cancer

Liu X,Zheng W,Wang W,Shen H,Liu L,Lou W,Wang X,Yang P

Tue Dec 05 00:00:00 EST 2017

A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.

British journal of cancer

Liu X,Zheng W,Wang W,Shen H,Liu L,Lou W,Wang X,Yang P

Tue Dec 05 00:00:00 EST 2017

Knockdown of ApoL1 in Zebrafish Larvae Affects the Glomerular Filtration Barrier and the Expression of Nephrin.

PloS one

Kotb AM,Simon O,Blumenthal A,Vogelgesang S,Dombrowski F,Amann K,Zimmermann U,Endlich K,Endlich N

Wed Jul 26 00:00:00 EDT 2017

Knockdown of ApoL1 in Zebrafish Larvae Affects the Glomerular Filtration Barrier and the Expression of Nephrin.

PloS one

Kotb AM,Simon O,Blumenthal A,Vogelgesang S,Dombrowski F,Amann K,Zimmermann U,Endlich K,Endlich N

Wed Jul 26 00:00:00 EDT 2017

BH3 domain-independent apolipoprotein L1 toxicity rescued by BCL2 prosurvival proteins.

American journal of physiology. Cell physiology

Heneghan JF,Vandorpe DH,Shmukler BE,Giovinazzo JA,Raper J,Friedman DJ,Pollak MR,Alper SL

Tue Sep 01 00:00:00 EDT 2015

BH3 domain-independent apolipoprotein L1 toxicity rescued by BCL2 prosurvival proteins.

American journal of physiology. Cell physiology

Heneghan JF,Vandorpe DH,Shmukler BE,Giovinazzo JA,Raper J,Friedman DJ,Pollak MR,Alper SL

Tue Sep 01 00:00:00 EDT 2015

Evolution of the primate trypanolytic factor APOL1.

Proceedings of the National Academy of Sciences of the United States of America

Thomson R,Genovese G,Canon C,Kovacsics D,Higgins MK,Carrington M,Winkler CA,Kopp J,Rotimi C,Adeyemo A,Doumatey A,Ayodo G,Alper SL,Pollak MR,Friedman DJ,Raper J

Tue May 20 00:00:00 EDT 2014

Evolution of the primate trypanolytic factor APOL1.

Proceedings of the National Academy of Sciences of the United States of America

Thomson R,Genovese G,Canon C,Kovacsics D,Higgins MK,Carrington M,Winkler CA,Kopp J,Rotimi C,Adeyemo A,Doumatey A,Ayodo G,Alper SL,Pollak MR,Friedman DJ,Raper J

Tue May 20 00:00:00 EDT 2014

Comparative proteomic profiling of plasma very-low-density and low-density lipoproteins.

Clinica chimica acta; international journal of clinical chemistry

Sun HY,Chen SF,Lai MD,Chang TT,Chen TL,Li PY,Shieh DB,Young KC

Mon Mar 01 00:00:00 EST 2010

Comparative proteomic profiling of plasma very-low-density and low-density lipoproteins.

Clinica chimica acta; international journal of clinical chemistry

Sun HY,Chen SF,Lai MD,Chang TT,Chen TL,Li PY,Shieh DB,Young KC

Mon Mar 01 00:00:00 EST 2010

Hydrodynamic gene delivery of baboon trypanosome lytic factor eliminates both animal and human-infective African trypanosomes.

Proceedings of the National Academy of Sciences of the United States of America

Thomson R,Molina-Portela P,Mott H,Carrington M,Raper J

Tue Nov 17 00:00:00 EST 2009

Hydrodynamic gene delivery of baboon trypanosome lytic factor eliminates both animal and human-infective African trypanosomes.

Proceedings of the National Academy of Sciences of the United States of America

Thomson R,Molina-Portela P,Mott H,Carrington M,Raper J

Tue Nov 17 00:00:00 EST 2009

Distinct roles of apolipoprotein components within the trypanosome lytic factor complex revealed in a novel transgenic mouse model.

The Journal of experimental medicine

Molina-Portela MP,Samanovic M,Raper J

Mon Aug 04 00:00:00 EDT 2008

Distinct roles of apolipoprotein components within the trypanosome lytic factor complex revealed in a novel transgenic mouse model.

The Journal of experimental medicine

Molina-Portela MP,Samanovic M,Raper J

Mon Aug 04 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: Apo-L; APOL- I; ApoL-I; Apolipoprotein L; apolipoprotein L 1; apolipoprotein L, 1; Apolipoprotein L-I; Apolipoprotein L1; OTTHUMP00000028705

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Gene Aliases: APO-L; APOL; APOL-I; APOL1; FSGS4

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UniProt ID: (Human) O14791

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Entrez Gene ID: (Human) 8542

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Function(s)

chloride channel activity protein binding lipid binding apolipoprotein

Process(es)

lipid transport receptor-mediated endocytosis cholesterol metabolic process cytolysis killing of cells of other organism lipoprotein metabolic process innate immune response chloride transmembrane transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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APOL1 Polyclonal Antibody

APOL1 Antibody (11486-2-AP) in ICC/IF

Product Details

11486-2-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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