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Invitrogen
APPL1 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
APPL1 Antibody (PA5-76172) in WB
Western blot was performed using Anti-APPL1 Rabbit Polyclonal Antibody (Product # PA5-76172) and a 79 kDa band corresponding to APPL1 was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of SW480 (Lane 1), MCF7 (Lane 2), HeLa (Lane 3), HEK-293 (Lane 4), Mouse Brain (Lane 5), Rat Brain (Lane 6) and Mouse Spleen (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry... View More
Product Details
PA5-76172
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein of human APPL1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2719899
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signaling and insulin signaling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adapter protein containing PH domain, PTB domain and leucine zipper motif 1; adaptor protein APPL like; adaptor protein containing pH domain, PTB domain and leucine zipper motif 1; adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1; AKT2 interactor; DCC-interacting protein 13-alpha; DIP13 alpha; Dip13-alpha; signaling adaptor protein DIP13alpha
Gene Aliases: 2900057D21Rik; 7330406P05Rik; AI585782; APPL; APPL1; AW209077; BB022931; C88264; DIP13; DIP13A; DIP13alpha; KIAA1428; MODY14; RGD1309388
UniProt ID: (Human) Q9UKG1, (Mouse) Q8K3H0
Entrez Gene ID: (Human) 26060, (Rat) 290537, (Mouse) 72993
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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