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APRIL Antibody (500-P192-1MG) in WB
Western Blot: To detect Human APRIL by Western Blot analysis APRIL Polyclonal Antibody (Product # 500-P192-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human APRIL is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P192-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
Insect cells-derived Recombinant Human APRIL
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929756
Product Specific Information
AA Sequence of recombinant protein: AVLTQKQKKQ HSVLHLVPIN ATSKDDSDVT EVMWQPALRR GRGLQAQGYG VRIQDAGVYL LYSQVLFQDV TFTMGQVVSR EGQGRQETLF RCIRSMPSHP DRAYNSCYSA GVFHLHQGDI LSVIIPRARA KLNLSPHGTF LGFVKL.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human APRIL. Anti-Human APRIL-specific antibody was purified by affinity chromatography employing an immobilized Human APRIL matrix.
Sandwich ELISA: To detect Human APRIL by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human APRIL (500-P192Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human APRIL.
Western Blot: To detect Human APRIL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human APRIL is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated APRIL (for A PRoliferation-Inducing Ligand), TALL2 (for TNF and ApoL-related Leukocyte-expressed Ligand 2), and TRDL 1a (for TNF related death ligand 1a) in human and mouse. Two receptors for APRIL were recently identified and designated TACI and BCMA. APRIL stimulates B and T cell proliferation, triggers humoral immune responses, activates NF-kB, and induces cell death. APRIL and its close relative of BlyS and their receptors BCMA and TACI are involved in diseases of autoimmunity and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A proliferation-inducing ligand; APRIL; CD256; TALL-2; TNF- and APOL-related leukocyte expressed ligand 2; TNF-related death ligand 1; TRDL-1; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand 7B; Tumor necrosis factor ligand superfamily member 13; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1
Gene Aliases: APRIL; CD256; TALL-2; TALL2; TNFSF13; TNLG7B; TRDL-1; UNQ383/PRO715; ZTNF2
UniProt ID: (Human) O75888
Entrez Gene ID: (Human) 8741
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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