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Product Details
H00000374-M06
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
3E4
Immunogen
AREG (AAH09799, 20 a.a. approximately 129 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate
Form
Liquid
Concentration
See Label
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Sequence of this protein is as follows: SGHYAAGLDL NDTYSGKREP FSGDHSADGF EVTSRSEMSS GSEISPVSEM PSSSEPSSGA DYDYSEEYDN EPQIPGYIVD DSVRVEQVVK PPQNKTESEN TSDKPKRKKK
Target Information
Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGFalpha, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Amphiregulin; amphiregulin B; AR; Colorectum cell-derived growth factor; CRDGF; MGC13647; schwannoma-derived growth factor
Gene Aliases: AR; AREG; AREGB; CRDGF; SDGF
UniProt ID: (Human) P15514
Entrez Gene ID: (Human) 374
ER to Golgi vesicle-mediated transport
epidermal growth factor receptor signaling pathway
G-protein coupled receptor signaling pathway
cell-cell signaling
cell proliferation
positive regulation of cell proliferation
glial cell proliferation
neuron projection development
response to estradiol
positive regulation of phosphorylation
response to hydrogen peroxide
response to peptide hormone
negative regulation of osteoblast differentiation
positive regulation of DNA replication
COPII vesicle coating
response to glucocorticoid
response to cAMP
dichotomous subdivision of terminal units involved in mammary gland duct morphogenesis
mammary gland branching involved in thelarche
mammary gland alveolus development
epithelial cell proliferation involved in mammary gland duct elongation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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