Invitrogen

ARF1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 Published Figures

5 References

View all (23) ARF1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

ARF1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

ARF1 Antibody (PA1-127) in ICC/IF

Immunofluorescence analysis of ARF1 was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ARF1 Rabbit Polyclonal Antibody(Product # PA1-127) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A270... View More

ARF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ARF1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

ARF1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with ARF1 siRNA and reduction of signal was observed in Western Blot using ARF1 Polyclonal Antibody (Product # PA1-127). {KD}

Product Details

PA1-127

Applications

Tested Dilution

Publications

Western Blot (WB)

1:3000

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunocytochemistry (ICC/IF)

5 µg/ml

Immunoprecipitation (IP)

2 µg

Product Specifications

Species Reactivity

Dog, Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide DWLSNQLRNQK

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539892

Product Specific Information

PA1-127 has been successfully used in Western blot in human, mouse, and canine samples, and in immunofluorescence and immunoprecipitation applications in human samples.

Target Information

ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Anti‑tumor properties of FoxO1 in YD‑9 oral squamous cell carcinoma cells.

Oncology reports

Kim YG,Seong C,Cho KA,Lee SM,Kim TJ,Kim HJ,Cho JH,Jung W,Jang S,Shin JC,Lee KH,Byun JS,Kim DY

PA1-127 was used in Western Blotting to demonstrate that FoxO1 exerted an anti‚tumor effect by suppressing proliferation and migration/invasion but promoting oxidative stress‚linked cell death in YD‚9 OSCC cells.

Thu Jun 01 00:00:00 EDT 2023

p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Science advances

Tan X,Banerjee P,Shi L,Xiao GY,Rodriguez BL,Grzeskowiak CL,Liu X,Yu J,Gibbons DL,Russell WK,Creighton CJ,Kurie JM

PA1-127 was used in Immunocytochemistry to show that p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Tue Jun 01 00:00:00 EDT 2021

p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Science advances

Tan X,Banerjee P,Shi L,Xiao GY,Rodriguez BL,Grzeskowiak CL,Liu X,Yu J,Gibbons DL,Russell WK,Creighton CJ,Kurie JM

PA1-127 was used in Immunocytochemistry to show that p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Tue Jun 01 00:00:00 EDT 2021

Targeting N-myristoylation for therapy of B-cell lymphomas.

Nature communications

Beauchamp E,Yap MC,Iyer A,Perinpanayagam MA,Gamma JM,Vincent KM,Lakshmanan M,Raju A,Tergaonkar V,Tan SY,Lim ST,Dong WF,Postovit LM,Read KD,Gray DW,Wyatt PG,Mackey JR,Berthiaume LG

Published figure using ARF1 polyclonal antibody (Product # PA1-127) in Western Blot

Thu Oct 22 00:00:00 EDT 2020

BIG1 controls macrophage pro-inflammatory responses through ARF3-mediated PI(4,5)P2 synthesis.

Cell death & disease

Liu L,Zhang S,Wang Y,Bao W,Zhou Y,Dang W,Wang X,Li H,Cao X,You Y,Fang H,Shen X

PA1-127 was used in Western Blotting to reveal a crucial new role of BIG1 in regulating macrophage inflammation responses, and provide evidence for BIG1 as a potential promising therapeutic target in sepsis.

Fri May 15 00:00:00 EDT 2020

Cocaine-induced endocannabinoid signaling mediated by sigma-1 receptors and extracellular vesicle secretion.

eLife

Nakamura Y,Dryanovski DI,Kimura Y,Jackson SN,Woods AS,Yasui Y,Tsai SY,Patel S,Covey DP,Su TP,Lupica CR

PA1-127 was used in Western Blot to implicate the Sig-1R-ARF6 complex in control of EV release and demonstrate that cocaine-mediated 2-AG release can occur via EVs.

Wed Oct 09 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: ADP-ribosylation factor 1

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Gene Aliases: ARF1

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UniProt ID: (Human) P84077, (Mouse) P84078

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Entrez Gene ID: (Human) 375, (Dog) 100856517, (Mouse) 11840

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Function(s)

magnesium ion binding GTPase activity receptor signaling protein activity protein binding GTP binding GDP binding poly(A) RNA binding phospholipase D activator activity nucleotide binding protein homodimerization activity G-protein

Process(es)

Performance Guarantee

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ARF1 Polyclonal Antibody

ARF1 Antibody (PA1-127) in ICC/IF

Product Details

PA1-127

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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