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ARF4 Antibody (PA5-119775) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of ARF4 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ARF4 Polyclonal Antibody (Product # PA5-119775) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).
Product Details
PA5-119775
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within C-terminal human ARF4
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2913347
Product Specific Information
Positive Control: HepG2 cell lysate, Hela cell lysate, mouse kidney tissue lysate, MCF-7 cell lysate, mouse cerebellum tissue lysate, rat testis tissue lysate, F9, human breast carcinoma tissue, mouse kidney tissue.
Subcellular Location: Golgi apparatus. Membrane.
Target Information
ADP-ribosylation factor 1 is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I and class III, and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADP-ribosylation factor 2; ADP-ribosylation factor 4
Gene Aliases: AA407803; ARF2; ARF4
UniProt ID: (Human) P18085, (Rat) P61751, (Mouse) P61750
Entrez Gene ID: (Human) 378, (Rat) 79120, (Mouse) 11843
protein ADP-ribosylation
ER to Golgi vesicle-mediated transport
retrograde vesicle-mediated transport, Golgi to ER
epidermal growth factor receptor signaling pathway
small GTPase mediated signal transduction
brain development
learning
protein transport
cell migration
activation of phospholipase D activity
negative regulation of apoptotic process
positive regulation of GTPase activity
apical protein localization
establishment or maintenance of epithelial cell apical/basal polarity
positive regulation of transcription from RNA polymerase II promoter
response to axon injury
dendritic spine development
protein localization to cilium
regulation of reactive oxygen species metabolic process
vesicle-mediated transport
transport
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