Proteintech

ASF/SF2 Polyclonal Antibody

ASF/SF2 Antibody in Immunocytochemistry (ICC/IF)

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ASF/SF2 Antibody (12929-2-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 12929-2-AP (ASF/SF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ASF/SF2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ASF/SF2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

12929-2-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 20 publications 20 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 4 publications 4 publications

RNA Immunoprecipitation (RIP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ASF/SF2 Fusion Protein Ag3626 (1-248 aa encoded by BC010264)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody can recognize all the 3 isoforms (28 kDa,33 kDa and 22 kDa) of SFRS1.

Immunogen sequence: MSGGGVIRG PAGNNDCRIY VGNLPPDIRT KDIEDVFYKY GAIRDIDLKN RRGGPPFAFV EFEDPRDAED AVYGRDGYDY DGYRLRVEFP RSGRGTGRGG GGGGGGGAPR GRYGPPSRRS ENRVVVSGLP PSGSWQDLKD HMREAGDVCY ADVYRDGTGV VEFVRKEDMT YAVRKLDNTK FRSHEGETAY IRVKVDGPRS PSYGRSRSRS RSRSRSRSRS NSRSRSYSPR RSRGSPRYSP RHSRSRSRT (1-248 aa encoded by BC010264 )

Target Information

UBE2E2 catalyzes the covalent attachment of ubiquitin to other proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

RNF125 attenuates hepatocellular carcinoma progression by downregulating SRSF1-ERK pathway.

Oncogene

Feng Z,Ke S,Wang C,Lu S,Xu Y,Yu H,Li Z,Yin B,Li X,Hua Y,Qian B,Bai M,Fu Y,Zhang Y,Wu Y,Ma Y

Thu Jun 01 00:00:00 EDT 2023

RNF125 attenuates hepatocellular carcinoma progression by downregulating SRSF1-ERK pathway.

Oncogene

Feng Z,Ke S,Wang C,Lu S,Xu Y,Yu H,Li Z,Yin B,Li X,Hua Y,Qian B,Bai M,Fu Y,Zhang Y,Wu Y,Ma Y

Thu Jun 01 00:00:00 EDT 2023

HLTF promotes hepatocellular carcinoma progression by enhancing SRSF1 stability and activating ERK/MAPK pathway.

Oncogenesis

Xu Y,Ke S,Lu S,Wang C,Li Z,Feng Z,Yu H,Bai M,Qian B,Yin B,Li X,Hua Y,Jiang H,Ma Y

Fri Jan 20 00:00:00 EST 2023

Photobiomodulation Drives MiR-136-5p Expression to Promote Injury Repair after Myocardial Infarction.

International journal of biological sciences

Gao X,Li H,Zhang W,Wang X,Sun H,Cao Y,Zhao Y,Ji H,Yang F,Ma W,Liu Y,Yang B,Cai B

Thu May 12 00:00:00 EDT 2022

Extracellular matrix stiffness controls VEGF₁₆₅ secretion and neuroblastoma angiogenesis via the YAP/RUNX2/SRSF1 axis.

Angiogenesis

Bao M,Chen Y,Liu JT,Bao H,Wang WB,Qi YX,Lv F

Tue Feb 01 00:00:00 EST 2022

Extracellular matrix stiffness controls VEGF₁₆₅ secretion and neuroblastoma angiogenesis via the YAP/RUNX2/SRSF1 axis.

Angiogenesis

Bao M,Chen Y,Liu JT,Bao H,Wang WB,Qi YX,Lv F

Tue Feb 01 00:00:00 EST 2022

circPRKAA1 activates a Ku80/Ku70/SREBP-1 axis driving de novo fatty acid synthesis in cancer cells.

Cell reports

Li Q,Yao H,Wang Y,Wu Y,Thorne RF,Zhu Y,Wu M,Liu L

Tue Nov 22 00:00:00 EST 2022

NSrp70 suppresses metastasis in triple-negative breast cancer by modulating Numb/TβR1/EMT axis.

Oncogene

Zhao Y,Sun H,Zhao Y,Liu Q,Liu Y,Hou Y,Jin W

Wed Jun 01 00:00:00 EDT 2022

NSrp70 suppresses metastasis in triple-negative breast cancer by modulating Numb/TβR1/EMT axis.

Oncogene

Zhao Y,Sun H,Zhao Y,Liu Q,Liu Y,Hou Y,Jin W

Wed Jun 01 00:00:00 EDT 2022

circSLC41A1 Resists Porcine Granulosa Cell Apoptosis and Follicular Atresia by Promoting SRSF1 through miR-9820-5p Sponging.

International journal of molecular sciences

Wang H,Zhang Y,Zhang J,Du X,Li Q,Pan Z

Fri Jan 28 00:00:00 EST 2022

LncRNA DACH1 protects against pulmonary fibrosis by binding to SRSF1 to suppress CTNNB1 accumulation.

Acta pharmaceutica Sinica. B

Sun J,Jin T,Niu Z,Guo J,Guo Y,Yang R,Wang Q,Gao H,Zhang Y,Li T,He W,Li Z,Ma W,Su W,Li L,Fan X,Shan H,Liang H

Thu Sep 01 00:00:00 EDT 2022

LncRNA DACH1 protects against pulmonary fibrosis by binding to SRSF1 to suppress CTNNB1 accumulation.

Acta pharmaceutica Sinica. B

Sun J,Jin T,Niu Z,Guo J,Guo Y,Yang R,Wang Q,Gao H,Zhang Y,Li T,He W,Li Z,Ma W,Su W,Li L,Fan X,Shan H,Liang H

Thu Sep 01 00:00:00 EDT 2022

Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.

Nucleic acids research

Khan M,Hou S,Azam S,Lei H

Mon Jun 21 00:00:00 EDT 2021

SRSF protein kinase 1 modulates RAN translation and suppresses CGG repeat toxicity.

EMBO molecular medicine

Malik I,Tseng YJ,Wright SE,Zheng K,Ramaiyer P,Green KM,Todd PK

Mon Nov 08 00:00:00 EST 2021

The SRSF1/circATP5B/miR-185-5p/HOXB5 feedback loop regulates the proliferation of glioma stem cells via the IL6-mediated JAK2/STAT3 signaling pathway.

Journal of experimental & clinical cancer research : CR

Zhao J,Jiang Y,Zhang H,Zhou J,Chen L,Li H,Xu J,Zhang G,Jing Z

Thu Apr 15 00:00:00 EDT 2021

The SRSF1/circATP5B/miR-185-5p/HOXB5 feedback loop regulates the proliferation of glioma stem cells via the IL6-mediated JAK2/STAT3 signaling pathway.

Journal of experimental & clinical cancer research : CR

Zhao J,Jiang Y,Zhang H,Zhou J,Chen L,Li H,Xu J,Zhang G,Jing Z

Thu Apr 15 00:00:00 EDT 2021

The long non-coding RNA PFI protects against pulmonary fibrosis by interacting with splicing regulator SRSF1.

Cell death and differentiation

Sun J,Jin T,Su W,Guo Y,Niu Z,Guo J,Li L,Wang J,Ma L,Yu T,Li X,Zhou Y,Shan H,Liang H

Fri Oct 01 00:00:00 EDT 2021

The long non-coding RNA PFI protects against pulmonary fibrosis by interacting with splicing regulator SRSF1.

Cell death and differentiation

Sun J,Jin T,Su W,Guo Y,Niu Z,Guo J,Li L,Wang J,Ma L,Yu T,Li X,Zhou Y,Shan H,Liang H

Fri Oct 01 00:00:00 EDT 2021

The long non-coding RNA PFI protects against pulmonary fibrosis by interacting with splicing regulator SRSF1.

Cell death and differentiation

Sun J,Jin T,Su W,Guo Y,Niu Z,Guo J,Li L,Wang J,Ma L,Yu T,Li X,Zhou Y,Shan H,Liang H

Fri Oct 01 00:00:00 EDT 2021

The long non-coding RNA PFI protects against pulmonary fibrosis by interacting with splicing regulator SRSF1.

Cell death and differentiation

Sun J,Jin T,Su W,Guo Y,Niu Z,Guo J,Li L,Wang J,Ma L,Yu T,Li X,Zhou Y,Shan H,Liang H

Fri Oct 01 00:00:00 EDT 2021

An APEX2 proximity ligation method for mapping interactions with the nuclear lamina.

The Journal of cell biology

Tran JR,Paulson DI,Moresco JJ,Adam SA,Yates JR,Goldman RD,Zheng Y

Mon Jan 04 00:00:00 EST 2021

An APEX2 proximity ligation method for mapping interactions with the nuclear lamina.

The Journal of cell biology

Tran JR,Paulson DI,Moresco JJ,Adam SA,Yates JR,Goldman RD,Zheng Y

Mon Jan 04 00:00:00 EST 2021

CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA-Binding Proteins and Sponging MiR-942.

Cancer research

Wong CH,Lou UK,Li Y,Chan SL,Tong JH,To KF,Chen Y

Mon Jun 01 00:00:00 EDT 2020

CD63⁺ Cancer-Associated Fibroblasts Confer Tamoxifen Resistance to Breast Cancer Cells through Exosomal miR-22.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Gao Y,Li X,Zeng C,Liu C,Hao Q,Li W,Zhang K,Zhang W,Wang S,Zhao H,Fan D,Li M,Zhang Y,Zhang W,Zhang C

Sun Nov 01 00:00:00 EDT 2020

CD63⁺ Cancer-Associated Fibroblasts Confer Tamoxifen Resistance to Breast Cancer Cells through Exosomal miR-22.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Gao Y,Li X,Zeng C,Liu C,Hao Q,Li W,Zhang K,Zhang W,Wang S,Zhao H,Fan D,Li M,Zhang Y,Zhang W,Zhang C

Sun Nov 01 00:00:00 EDT 2020

LncRNA HOTAIR aggravates myocardial ischemia-reperfusion injury by sponging microRNA-126 to upregulate SRSF1.

European review for medical and pharmacological sciences

Sun Y,Hu ZQ

Tue Sep 01 00:00:00 EDT 2020

SFRS11 Loss Leads to Aging-Associated Cognitive Decline by Modulating LRP8 and ApoE.

Cell reports

Raihan O,Brishti A,Li Q,Zhang Q,Li D,Li X,Zhang Q,Xie Z,Li J,Zhang J,Liu Q

Tue Jul 02 00:00:00 EDT 2019

Long noncoding RNA HOXA11-AS promotes gastric cancer cell proliferation and invasion via SRSF1 and functions as a biomarker in gastric cancer.

World journal of gastroenterology

Liu Y,Zhang YM,Ma FB,Pan SR,Liu BZ

Fri Jun 14 00:00:00 EDT 2019

Zc3h13 Regulates Nuclear RNA m⁶A Methylation and Mouse Embryonic Stem Cell Self-Renewal.

Molecular cell

Wen J,Lv R,Ma H,Shen H,He C,Wang J,Jiao F,Liu H,Yang P,Tan L,Lan F,Shi YG,He C,Shi Y,Diao J

Thu Mar 15 00:00:00 EDT 2018

Zc3h13 Regulates Nuclear RNA m⁶A Methylation and Mouse Embryonic Stem Cell Self-Renewal.

Molecular cell

Wen J,Lv R,Ma H,Shen H,He C,Wang J,Jiao F,Liu H,Yang P,Tan L,Lan F,Shi YG,He C,Shi Y,Diao J

Thu Mar 15 00:00:00 EDT 2018

Defensive effect of microRNA-200b/c against amyloid-beta peptide-induced toxicity in Alzheimer's disease models.

PloS one

Higaki S,Muramatsu M,Matsuda A,Matsumoto K,Satoh JI,Michikawa M,Niida S

Wed Aug 01 00:00:00 EDT 2018

Defensive effect of microRNA-200b/c against amyloid-beta peptide-induced toxicity in Alzheimer's disease models.

PloS one

Higaki S,Muramatsu M,Matsuda A,Matsumoto K,Satoh JI,Michikawa M,Niida S

Wed Aug 01 00:00:00 EDT 2018

Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth.

Nature communications

Chen Y,Huang Q,Liu W,Zhu Q,Cui CP,Xu L,Guo X,Wang P,Liu J,Dong G,Wei W,Liu CH,Feng Z,He F,Zhang L

Mon Jun 25 00:00:00 EDT 2018

Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth.

Nature communications

Chen Y,Huang Q,Liu W,Zhu Q,Cui CP,Xu L,Guo X,Wang P,Liu J,Dong G,Wei W,Liu CH,Feng Z,He F,Zhang L

Mon Jun 25 00:00:00 EDT 2018

PP1γ functionally augments the alternative splicing of CaMKIIδ through interaction with ASF.

American journal of physiology. Cell physiology

Huang C,Cao W,Liao R,Wang J,Wang Y,Tong L,Chen X,Zhu W,Zhang W

Wed Jan 15 00:00:00 EST 2014

PP1γ functionally augments the alternative splicing of CaMKIIδ through interaction with ASF.

American journal of physiology. Cell physiology

Huang C,Cao W,Liao R,Wang J,Wang Y,Tong L,Chen X,Zhu W,Zhang W

Wed Jan 15 00:00:00 EST 2014

Rescue of cardiac failing and remodelling by inhibition of protein phosphatase 1γ is associated with suppression of the alternative splicing factor-mediated splicing of Ca2+/calmodulin-dependent protein kinase δ.

Clinical and experimental pharmacology & physiology

Liao RJ,Tong LJ,Huang C,Cao WW,Wang YZ,Wang J,Chen XF,Zhu WZ,Zhang W

Mon Dec 01 00:00:00 EST 2014

Clinical and experimental pharmacology & physiology

Liao RJ,Tong LJ,Huang C,Cao WW,Wang YZ,Wang J,Chen XF,Zhu WZ,Zhang W

Mon Dec 01 00:00:00 EST 2014

Bioinformatics

Protein Aliases: Alternative-splicing factor 1; ASF-1; ASF/SF2; pre-mRNA-splicing factor SF2, P33 subunit; Pre-mRNA-splicing factor SRp30a; Serine/arginine-rich splicing factor 1; splicing factor 2; Splicing factor, arginine/serine-rich 1; splicing factor, arginine/serine-rich 1 (ASF/SF2); splicing factor, arginine/serine-rich 1B; splicing factor, arginine/serine-rich, 30-KD, A; SR splicing factor 1

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Gene Aliases: 1110054N12Rik; 5730507C05Rik; 6330415C05Rik; AI482334; ASF; AW491331; OK/SW-cl.3; SF2; SF2P33; SFRS1; SRp30a; SRSF1

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UniProt ID: (Human) Q07955, (Mouse) Q5SXC5

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Entrez Gene ID: (Human) 6426, (Rat) 689890, (Mouse) 110809

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Function(s)

nucleotide binding RNA binding mRNA binding protein binding poly(A) RNA binding RS domain binding nucleic acid binding RNA splicing factor

Process(es)

alternative mRNA splicing, via spliceosome mRNA 5'-splice site recognition mRNA splicing, via spliceosome in utero embryonic development termination of RNA polymerase II transcription mRNA splice site selection mRNA processing RNA export from nucleus mRNA export from nucleus mRNA 3'-end processing cardiac muscle contraction regulation of transcription, DNA-templated regulation of translation RNA splicing regulation of mRNA stability regulation of mRNA splicing, via spliceosome transport mRNA transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ASF/SF2 Polyclonal Antibody

ASF/SF2 Antibody (12929-2-AP) in ICC/IF

Product Details

12929-2-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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