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ASF1B Antibody (CF813126) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASF1B (RC206114, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASF1B (1:500).
Product Details
CF813126
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
OTI1H5
Immunogen
Full length human recombinant protein of human ASF1B (NP_060624) produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 8% trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Anti-silencing function protein 1 homolog B; ASF1 anti-silencing function 1 homolog B; CCG1-interacting factor A-II; CIA-II; hAsf1; hAsf1b; hCIA-II; Histone chaperone ASF1B; mCIA-II
Gene Aliases: 1700003K02Rik; AA409591; ASF1B; CIA-II
UniProt ID: (Human) Q9NVP2, (Mouse) Q9DAP7
Entrez Gene ID: (Human) 55723, (Rat) 304648, (Mouse) 66929
DNA replication-dependent nucleosome assembly
DNA replication-independent nucleosome assembly
transcription, DNA-templated
regulation of transcription, DNA-templated
multicellular organism development
spermatogenesis
covalent chromatin modification
cell differentiation
nucleosome assembly
chromatin assembly or disassembly
chromatin modification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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