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ASH2L Antibody (PA5-119994) in IHC (P)
Immunohistochemistry (Paraffin) analysis of paraffin-embedded human breast carcinoma tissue using ASH2L Polyclonal Antibody (Product # PA5-119994). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the ASH2L antibody at a d... View More
Product Details
PA5-119994
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within human ASH2L aa 575-628
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2913566
Product Specific Information
Positive Control: Hela cell lysates, PC-12 cell lysates, mouse colon tissue, human breast carcinoma tissue, human colon tissue, Siha.
Subcellular Location: Nucleus.
Target Information
The human ASH2L gene encodes a 628 amino acid protein known as ASH2L1, or isoform 1, which contains a nuclear localization signal and PHD finger motif, suggesting that the gene product functions as a transcription regulator. Its predicted molecular weight is 68.7kDa. Alternative splicing results in possible isoforms at 60 kDa & 56.5 kDa). Human ASH2L proteins are 60% homologous to Drosophila ash2, which positively regulates expression of certain genes in early development, and contain similar, but not identical, domains, including a zinc finger motif. Homology to mouse ASH2L is 90%. ASH2L is predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes as well as leukemia cell lines with erythroid and megakaryocytic potential, such as K562, Hel and Dami. Differentiation inducers (e.g. phorbol ester and hemin) cause different expression patterns in these cell lines, suggesting that ASH2L plays a role in hematopoiesis and is associated with particular types of leukemia. In addition ASH2L has been suggested to interact with beta-tubulin and beta-catenin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ASH 2; ash2 (absent, small, or homeotic)-like; ASH2 LIKE; ASH2 like protein; ASH2-like protein; Bre 2; Set1/Ash2 histone methyltransferase complex subunit ASH2
Gene Aliases: ASH2; ASH2L; ASH2L1; ASH2L2; Bre2
UniProt ID: (Human) Q9UBL3, (Mouse) Q91X20
Entrez Gene ID: (Human) 9070, (Mouse) 23808, (Rat) 290829
DNA binding
protein binding
beta-catenin binding
histone-lysine N-methyltransferase activity
histone methyltransferase activity (H3-K4 specific)
transcription regulatory region DNA binding
metal ion binding
euchromatin binding
chromatin/chromatin-binding, or -regulatory protein
histone modifying enzyme
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
positive regulation of cell proliferation
hemopoiesis
response to estrogen
positive regulation of transcription from RNA polymerase II promoter
histone H3-K4 methylation
beta-catenin-TCF complex assembly
chromatin modification
chromatin-mediated maintenance of transcription
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