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ASH2L Antibody (14-5069-82) in ICC/IF
Immunofluorescent analysis of ASH2L (green) in HeLa cells (65% confluency). The cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 1% Triton X-100 for 30 minutes at 37 C, and blocked with 10% Normal Goat Serum in PBS with 0.1% Triton X-100 for 1 hour at room temperature. Cells were stained overnight with the ASH2L mouse monoclonal antibody (Product # 14-5069-82) at 4 C at a concentration of 1.25 µg/mL in blocking buffer followed by a Goat-anti Rat IgG Secondary Antibody, Alexa Fluor 488 (Produc... View More
Product Details
14-5069-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
AS 4C5
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
purified
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2762677
Product Specific Information
This AS 4C5 monoclonal antibody recognizes human ASH2L, which has been described as a transcriptional regulator and may play a role in hematopoiesis. AS 4C5 detects full length (unspliced) and reduced ASH2L by Western Blot and stains ASH2L in the nucleoplasm and at the plasma membrane in Immunocytochemistry. ASH2L monoclonal antibody (AS 4C5) has been validated for the following applications: Western Blot and Immunocytochemistry; AS 4C5 does not cross react with mouse ASH2L.
Applications Reported: This AS 4C5 antibody has been reported for use in Western Blot and Immunocytochemistry.
Applications Tested: This AS 4C5 antibody has been tested by western blot using whole cell lysates of HeLa cells (human) and NIH 3T3 or N2a (mouse cell lines) and by immunocytochemistry on HeLa cells. This AS 4C5 antibody may be used at 2 µg/mL for western blot and at 1.25 µg/mL for immunocytochemistry.
Target Information
The human ASH2L gene encodes a 628 amino acid protein known as ASH2L1, or isoform 1, which contains a nuclear localization signal and PHD finger motif, suggesting that the gene product functions as a transcription regulator. Its predicted molecular weight is 68.7kDa. Alternative splicing results in possible isoforms at 60 kDa & 56.5 kDa). Human ASH2L proteins are 60% homologous to Drosophila ash2, which positively regulates expression of certain genes in early development, and contain similar, but not identical, domains, including a zinc finger motif. Homology to mouse ASH2L is 90%. ASH2L is predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes as well as leukemia cell lines with erythroid and megakaryocytic potential, such as K562, Hel and Dami. Differentiation inducers (e.g. phorbol ester and hemin) cause different expression patterns in these cell lines, suggesting that ASH2L plays a role in hematopoiesis and is associated with particular types of leukemia. In addition ASH2L has been suggested to interact with beta-tubulin and beta-catenin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ASH 2; ash2 (absent, small, or homeotic)-like; ASH2 LIKE; ASH2 like protein; ASH2-like protein; Bre 2; Set1/Ash2 histone methyltransferase complex subunit ASH2
Gene Aliases: ASH2; ASH2L; ASH2L1; ASH2L2; Bre2
UniProt ID: (Human) Q9UBL3
Entrez Gene ID: (Human) 9070
DNA binding
protein binding
beta-catenin binding
histone-lysine N-methyltransferase activity
histone methyltransferase activity (H3-K4 specific)
transcription regulatory region DNA binding
metal ion binding
euchromatin binding
chromatin/chromatin-binding, or -regulatory protein
histone modifying enzyme
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
positive regulation of cell proliferation
hemopoiesis
response to estrogen
positive regulation of transcription from RNA polymerase II promoter
histone H3-K4 methylation
beta-catenin-TCF complex assembly
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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