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Product Details
PA5-142745
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide with sequence C-DKKRTEYLPAHEDRT.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2933391
Product Specific Information
Additional Information: This antibody is expected to recognize both reported isoforms (NP_038754.1; NP_001177646.1).
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:16,000.
Target Information
Activator of S phase kinase (ASK, DBF4) is a novel regulatory subunit of the human Cdc7 kinase complex that plays a pivotal role in G1/S cell cycle transition in mammalian cells. The increase in Cdc7 kinase activity at the G1/S boundary is at least partly accounted for by the elevated expression of ASK in late G1. ASK interacts with replication origins in vivo, suggesting that Cdc7 may trigger S phase by directly activating the replication initiation complexes assembled at the origins. Human ASK protein levels are regulated during the cell cycle with a pattern that matches that of human Cdc7 protein kinase activity. Human Cdc7-ASK kinase is directly involved in regulating the initiation of DNA replication by targeting MCM2 protein in mammalian cells. Nuclear localization and chromatin binding of endogenous huCdc7 and GFP-ASK is presumably imported into nuclei through its two nuclear localization signals at telophase, it may require additional signals for chromatin binding, the level of which increases at late G1 ohase. A functional link may exist between menin, a tumor suppressor protein mutated in multiple endocrine neoplasia type I (MEN1), and ASK in the regulation of cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: activator of S phase kinase; DBF4 homolog; MuDBF4; Protein DBF4 homolog A
Gene Aliases: AA545217; Ask; Dbf4; Dbf4a; RGD1305854
UniProt ID: (Mouse) Q9QZ41
Entrez Gene ID: (Mouse) 27214, (Rat) 312046
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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