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ASPP2 Antibody (200-401-A19) in WB
Western blot using Rocklands Protein A purified anti-ASPP2 to detect over-expressed ASPP2 in MCF-7 cells (Lane 2, arrowhead). Lane 1 is a nontransfected control. Lane 3 is MCF-7 cells over-expressing ASPP1. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1,000 dilution. The identity of the lower MW band at approximately 40kDa is unknown. Personal Communication, H. Yang, Univ. Oklahoma, Oklahoma City, OK.
Product Details
200-401-A19
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human ASPP2.
Conjugate
Form
Liquid
Concentration
2.4 mg/mL
Purification
Protein A
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Directed against human ASPP2. The product was purified from monospecific antiserum by Protein A affinity chromatography. Minimal reactivity occurs against ASPP1. A BLAST analysis was used to suggest cross-reactivity with ASPP2 from macaque and dog sources based on a 100% homology with the immunizing sequence. Expect partial reactivity with ASPP2 from mouse and rat sources based on an 80% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Target Information
Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 53BP2; Apoptosis-stimulating of p53 protein 2; apoptosis-stimulating protein of p53, 2; Bbp; BCL2-binding protein; P PPP1R13A; Renal carcinoma antigen NY-REN-51; tumor protein p53 binding protein, 2; Tumor suppressor p53-binding protein 2
Gene Aliases: 53BP2; ASPP2; BBP; P53BP2; PPP1R13A; TP53BP2
UniProt ID: (Human) Q13625
Entrez Gene ID: (Human) 7159
cell cycle
signal transduction
regulation of apoptotic process
negative regulation of cell cycle
intrinsic apoptotic signaling pathway by p53 class mediator
positive regulation of execution phase of apoptosis
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
positive regulation of neuron death
regulation of signal transduction by p53 class mediator
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