Proteintech

ATF2 Polyclonal Antibody

ATF2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ATF2 Antibody (14834-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using (Product # 14834-1-AP) (ATF2-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

14834-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human ATF2. The calculated molecular weight of ATF2 is 55 kDa, but the modified ATF2 protein is about 70 kDa.

Target Information

The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Hepatocyte-derived MASP1-enriched small extracellular vesicles activate HSCs to promote liver fibrosis.

Hepatology (Baltimore, Md.)

Liu X,Tan S,Liu H,Jiang J,Wang X,Li L,Wu B

Sat Apr 01 00:00:00 EDT 2023

A druggable copper-signalling pathway that drives inflammation.

Nature

Solier S,Müller S,Cañeque T,Versini A,Mansart A,Sindikubwabo F,Baron L,Emam L,Gestraud P,Pantoș GD,Gandon V,Gaillet C,Wu TD,Dingli F,Loew D,Baulande S,Durand S,Sencio V,Robil C,Trottein F,Péricat D,Näser E,Cougoule C,Meunier E,Bègue AL,Salmon H,Manel N,Puisieux A,Watson S,Dawson MA,Servant N,Kroemer G,Annane D,Rodriguez R

Mon May 01 00:00:00 EDT 2023

Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis.

BMC medicine

Zheng Z,Wu M,Li H,Xu W,Yang M,Pan K,Ni Y,Jiang T,Zheng H,Jin X,Zhang Y,Ding L,Fu J

Mon Apr 03 00:00:00 EDT 2023

Echinococcus multilocularis drives the polarization of macrophages by regulating the RhoA-MAPK signaling pathway and thus affects liver fibrosis.

Bioengineered

Chong S,Chen G,Dang Z,Niu F,Zhang L,Ma H,Zhao Y

Fri Apr 01 00:00:00 EDT 2022

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Nature

Burda JE,O'Shea TM,Ao Y,Suresh KB,Wang S,Bernstein AM,Chandra A,Deverasetty S,Kawaguchi R,Kim JH,McCallum S,Rogers A,Wahane S,Sofroniew MV

Wed Jun 01 00:00:00 EDT 2022

Effect of resistance training on satellite cells in old mice - a transcriptome study : implications for sarcopenia.

Bone & joint research

Hsu WB,Lin SJ,Hung JS,Chen MH,Lin CY,Hsu WH,Hsu WR

Tue Feb 01 00:00:00 EST 2022

SET8 participates in lipopolysaccharide-mediated BV2 cell inflammation via modulation of TICAM-2 expression.

Canadian journal of physiology and pharmacology

Zhao Y,Yang X,Meng F,Li W

Sun Nov 01 00:00:00 EDT 2020

Molecular mechanism of miR-204 regulates proliferation, apoptosis and autophagy of cervical cancer cells by targeting ATF2.

Artificial cells, nanomedicine, and biotechnology

Li N,Guo X,Liu L,Wang L,Cheng R

Sun Dec 01 00:00:00 EST 2019

Maternal Protein Restriction Induces Alterations in Hepatic Unfolded Protein Response-Related Molecules in Adult Rat Offspring.

Frontiers in endocrinology

Liu X,Wang J,Gao L,Jiao Y,Liu C

Wed Nov 20 00:00:00 EST 2019

Maternal Protein Restriction Induces Alterations in Hepatic Unfolded Protein Response-Related Molecules in Adult Rat Offspring.

Frontiers in endocrinology

Liu X,Wang J,Gao L,Jiao Y,Liu C

Wed Nov 20 00:00:00 EST 2019

Cold-Inducible SIRT6 Regulates Thermogenesis of Brown and Beige Fat.

Cell reports

Yao L,Cui X,Chen Q,Yang X,Fang F,Zhang J,Liu G,Jin W,Chang Y

Tue Jul 18 00:00:00 EDT 2017

Cold-Inducible SIRT6 Regulates Thermogenesis of Brown and Beige Fat.

Cell reports

Yao L,Cui X,Chen Q,Yang X,Fang F,Zhang J,Liu G,Jin W,Chang Y

Tue Jul 18 00:00:00 EDT 2017

ATF2 predicts poor prognosis and promotes malignant phenotypes in renal cell carcinoma.

Journal of experimental & clinical cancer research : CR

Wu DS,Chen C,Wu ZJ,Liu B,Gao L,Yang Q,Chen W,Chen JM,Bao Y,Qu L,Wang LH

Mon Jul 04 00:00:00 EDT 2016

ATF2 predicts poor prognosis and promotes malignant phenotypes in renal cell carcinoma.

Journal of experimental & clinical cancer research : CR

Wu DS,Chen C,Wu ZJ,Liu B,Gao L,Yang Q,Chen W,Chen JM,Bao Y,Qu L,Wang LH

Mon Jul 04 00:00:00 EDT 2016

MicroRNA-26b inhibits hepatitis B virus transcription and replication by targeting the host factor CHORDC1 protein.

The Journal of biological chemistry

Zhao F,Xu G,Zhou Y,Wang L,Xie J,Ren S,Liu S,Zhu Y

Fri Dec 12 00:00:00 EST 2014

MicroRNA-26b inhibits hepatitis B virus transcription and replication by targeting the host factor CHORDC1 protein.

The Journal of biological chemistry

Zhao F,Xu G,Zhou Y,Wang L,Xie J,Ren S,Liu S,Zhu Y

Fri Dec 12 00:00:00 EST 2014

Bioinformatics

Protein Aliases: Activating 2; Activating transcription factor 2; activating transcription factor 2 splice variant ATF2-var2; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2, formerly; cAMP-dependent transcription factor ATF-2; CREB-2; Cyclic AMP-dependent transcription factor ATF-2; Cyclic AMP-responsive element-binding protein 2; HB16; histone acetyltransferase ATF2; MGC111558; MXBP protein; OTTHUMP00000205345; OTTHUMP00000205347; OTTHUMP00000205348; OTTHUMP00000205350; OTTHUMP00000205352; OTTHUMP00000205353; OTTHUMP00000205354

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Gene Aliases: Atf-2; ATF2; CRE-BP; CRE-BP1; CREB-2; CREB2; CREBP1; D130078H02Rik; D18875; HB16; mXBP; Tg(Gzma-Klra1)7Wum; TREB7

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UniProt ID: (Human) P15336, (Mouse) P16951

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Entrez Gene ID: (Human) 1386, (Mouse) 11909

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity histone acetyltransferase activity protein binding cAMP response element binding protein binding protein kinase binding cAMP response element binding metal ion binding protein heterodimerization activity nucleic acid binding DNA binding transferase activity sequence-specific DNA binding basic leucine zipper transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter outflow tract morphogenesis regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter response to osmotic stress cellular response to DNA damage stimulus response to water deprivation histone acetylation intra-S DNA damage checkpoint positive regulation of transforming growth factor beta2 production positive regulation of neuron apoptotic process fat cell differentiation positive regulation of transcription from RNA polymerase II promoter negative regulation of epithelial cell proliferation regulation of sequence-specific DNA binding transcription factor activity positive regulation of sequence-specific DNA binding transcription factor activity adipose tissue development amelogenesis positive regulation of mitochondrial membrane permeability involved in apoptotic process transcription, DNA-templated

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ATF2 Polyclonal Antibody

ATF2 Antibody (14834-1-AP) in IHC (P)

Product Details

14834-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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