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ATF4 Antibody (PA5-72620) in WB
Western blot analysis of ATF4 in various lysates. Samples were incubated with ATF4 polyclonal antibody (Product # PA5-72620) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: Zebrafish lysate; Lane 2: Zebrafish muscle lysate. Predicted band size: 37 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Product Details
PA5-72620
Product Specifications
Species Reactivity
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 288-314 amino acids from the C-terminal region of DANRE atf4
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2718474
Target Information
This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activating transcription factor 4; activating transcription factor 4 (tax-responsive enhancer element B67); activating transcription factor 4b1 (tax-responsive enhancer element B67); ATF 4; ATF4 protein; cAMP-dependent transcription factor ATF-4; CREB 2; Cyclic AMP-dependent transcription factor ATF-4; OTTHUMP00000199130; tax-re
Gene Aliases: atf4; atf4a; atf4b1; wu:fj01c08; wu:fk30b04; zgc:85951
UniProt ID: (Zebrafish) Q6NW59
Entrez Gene ID: (Zebrafish) 406514
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
cAMP response element binding protein binding
protein heterodimerization activity
DNA-binding transcription factor
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
bone mineralization
endoplasmic reticulum unfolded protein response
cellular response to amino acid starvation
cellular response to oxidative stress
embryonic hemopoiesis
positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress
PERK-mediated unfolded protein response
positive regulation of apoptotic process
regulation of osteoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
regulation of synaptic plasticity
rhythmic process
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
lens fiber cell morphogenesis
positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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