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ATG10 Antibody (PA5-96662) in IHC (P)
Immunohistochemistry analysis of ATG10 in paraffin-embedded mouse spleen. Samples were incubated with ATG10 Polyclonal antibody (Product # PA5-96662) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
PA5-96662
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 120-220 of human ATG10 (NP_1136701)
Conjugate
Form
Liquid
Concentration
2.13 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808464
Product Specific Information
Positive Samples: Mouse small intestine; Cellular Location: Cytoplasm
Immunogen sequence: LPLSYAKATS QDERNVP
Target Information
ATG10 is involved in autophagy which is a process for the bulk degradation of cytosolic compartments by lysosomes/vacuoles. The formation of autophagosomes involves a dynamic rearrangement of the membrane for which two ubiquitin-like modifications (the conjugation of APG12p and the modification of a soluble form of MAP-LC3 to a membrane-bound form) are essential. APG10 is an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation, ATG12 -ATG5 conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A), a homolog of yeast Apg8, to a membrane-bound form. The protein ATG10 is ubiquitously expressed and the human APG10L gene is localized on chromosome 5q14.2.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APG10-like; ATG10 autophagy related 10 homolog; autophagy related 10 homolog; autophagy-related 10; Autophagy-related protein 10; DKFZp586I0418; FLJ13954; mAPG10; Ubiquitin-like-conjugating enzyme ATG10
Gene Aliases: 5330424L23Rik; 5430428K15Rik; Agp10; AI852123; APG10; APG10L; Apg10p; ATG10; Atg10l; PP12616
UniProt ID: (Human) Q9H0Y0, (Mouse) Q8R1P4
Entrez Gene ID: (Human) 83734, (Rat) 688555, (Mouse) 66795
ligase activity
Atg12 transferase activity
molecular_function
protein binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
protein modifying enzyme
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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