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ATG5 Antibody (PA1-18285) in ICC/IF
Immunofluorescent analysis of APG5 (red) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with an APG5 polyclonal antibody (Product # PA1-18285) at a dilution of 1:20 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) we... View More
Product Details
PA1-18285
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the C-terminal residues of human APG-5L.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2062336
Product Specific Information
Reconstitute with 500 µL of distilled water. After reconstitution, store aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent.
Target Information
ATG5 (Autophagy Related 5) is an important element for autophagy and may play an important role in the apoptotic process. ATG5 is also involved in other cellular processes that include mitochondrial quality control after oxidative damage, negative regulation of the innate anti-viral immune response, lymphocyte development and proliferation, MHC II antigen presentation, and adipocyte differentiation. Following conjugation to ATG12, the conjugate participates in the formation of autophagosome. ATG5 contributes to autophagic cell death by interacting with Fas-associated protein with death domain (FADD). The ATG5-ATG12 conjugate forms a cup-shaped isolation membrane that then detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may play a role in the apoptotic process, possibly within the modified cytoskeleton. Further, APG5 expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. The APG5-APG12 conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. Diseases associated with ATG5 dysfunction include spinocerebellar ataxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APG 5; APG5 autophagy 5 like (APG5L); APG5-like; Apoptosis-specific protein; Apoptosis-specific protein (ASP); ATG5 autophagy related 5 homolog; Autophagy protein 5
Gene Aliases: APG5; APG5-LIKE; APG5L; ASP; ATG5; hAPG5
UniProt ID: (Human) Q9H1Y0
Entrez Gene ID: (Human) 9474
autophagosome assembly
mitophagy
blood vessel remodeling
regulation of cytokine secretion involved in immune response
C-terminal protein lipidation
autophagy
apoptotic process
cellular response to nitrogen starvation
response to fungus
macroautophagy
cellular homeostasis
antigen processing and presentation of endogenous antigen
negative regulation of protein ubiquitination
aggrephagy
negative stranded viral RNA replication
vasodilation
response to drug
negative regulation of apoptotic process
post-translational protein modification
nucleophagy
negative thymic T cell selection
innate immune response
otolith development
regulation of release of sequestered calcium ion into cytosol
ventricular cardiac muscle cell development
heart contraction
negative regulation of cell death
protein lipidation involved in autophagosome assembly
positive regulation of mucus secretion
regulation of cilium assembly
positive regulation of mitophagy
negative regulation of reactive oxygen species metabolic process
negative regulation of histone H4-K16 acetylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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