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ATG5 Antibody (PA1-46178) in ICC/IF
Immunofluorescence analysis of ATG5 was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ATG5 Rabbit Polyclonal Antibody (Product # PA1-46178) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More
Product Details
PA1-46178
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Fruit fly,
Fish,
Guinea pig,
Human,
Mouse,
Non-human primate,
Pig,
Reptile,
Rat,
Xenopus,
Zebrafish
Published species
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide made to an N-terminal region of the human ATG5 protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2274520
Product Specific Information
In Western Blot, a band is seen ~56 kDa representing the ATG5-ATG12 complex, the molecular weight of human ATG5 is ~33 kDa.
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Suggested positive control: antigen standard for ATG5 (transient overexpression lysate), mouse wildtype ES cell lysate.
Target Information
ATG5 (Autophagy Related 5) is an important element for autophagy and may play an important role in the apoptotic process. ATG5 is also involved in other cellular processes that include mitochondrial quality control after oxidative damage, negative regulation of the innate anti-viral immune response, lymphocyte development and proliferation, MHC II antigen presentation, and adipocyte differentiation. Following conjugation to ATG12, the conjugate participates in the formation of autophagosome. ATG5 contributes to autophagic cell death by interacting with Fas-associated protein with death domain (FADD). The ATG5-ATG12 conjugate forms a cup-shaped isolation membrane that then detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may play a role in the apoptotic process, possibly within the modified cytoskeleton. Further, APG5 expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. The APG5-APG12 conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. Diseases associated with ATG5 dysfunction include spinocerebellar ataxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: APG 5; APG5 autophagy 5 like (APG5L); APG5-like; Apoptosis-specific protein; Apoptosis-specific protein (ASP); ATG5 autophagy related 5 homolog; Atg5-PA; autophagy 5-like protein; Autophagy protein 5; autophagy related 5-like protein; autophagy-related 5; autophagy-specific gene 5; CG1643-PA; LD34980p
Gene Aliases: 2010107M05Rik; 3110067M24Rik; APG5; APG5-LIKE; APG5L; ASP; Atg-5; ATG5; Atg5l; AW319544; C88337; CG1643; DmAtg5; Dmel\CG1643; Dmel_CG1643; hAPG5; Paddy; XELAEV_18027227mg; zgc:100934
UniProt ID: (Pig) Q3MQ04, (Human) Q9H1Y0, (Bovine) Q3MQ24, (Fruit fly) Q9W3R7, (Mouse) Q99J83
immune system process
autophagy
apoptotic process
regulation of cilium assembly
autophagosome assembly
mitophagy
blood vessel remodeling
regulation of cytokine secretion involved in immune response
C-terminal protein lipidation
cellular response to nitrogen starvation
response to fungus
macroautophagy
cellular homeostasis
antigen processing and presentation of endogenous antigen
negative regulation of protein ubiquitination
aggrephagy
negative stranded viral RNA replication
vasodilation
response to drug
negative regulation of apoptotic process
post-translational protein modification
nucleophagy
negative thymic T cell selection
innate immune response
otolith development
regulation of release of sequestered calcium ion into cytosol
ventricular cardiac muscle cell development
heart contraction
negative regulation of cell death
protein lipidation involved in autophagosome assembly
positive regulation of mucus secretion
positive regulation of mitophagy
negative regulation of reactive oxygen species metabolic process
negative regulation of histone H4-K16 acetylation
negative regulation of phagocytosis
cellular response to nitrosative stress
autophagy of host cells involved in interaction with symbiont
cellular response to starvation
larval midgut cell programmed cell death
glycophagy
regulation of reactive oxygen species metabolic process
nervous system development
heart development
regulation of skeletal muscle tissue regeneration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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