Proteintech

ATG5 Monoclonal Antibody (2D3B8)

ATG5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ATG5 Antibody (66744-1-IG) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using (Product # 66744-1-IG) (ATG5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

66744-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 24 publications 24 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:300

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

2D3B8

Immunogen

ATG5 Fusion Protein Ag16685 (28-275 aa encoded by BC002699)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: TEREAEPYY LLLPRVSYLT LVTDKVKKHF QKVMRQEDIS EIWFEYEGTP LKWHYPIGLL FDLLASSSAL PWNITVHFKS FPEKDLLHCP SKDAIEAHFM SCMKEADALK HKSQVINEMQ KKDHKQLWMG LQNDRFDQFW AINRKLMEYP AEENGFRYIP FRIYQTTTER PFIQKLFRPV AADGQLHTLG DLLKEVCPSA IDPEDGEKKN QVMIHGIEPM LETPLQWLSE HLSYPDNFLH ISIIPQPTD (28-275 aa encoded by BC002699)

Target Information

ATG5 (Autophagy Related 5) is an important element for autophagy and may play an important role in the apoptotic process. ATG5 is also involved in other cellular processes that include mitochondrial quality control after oxidative damage, negative regulation of the innate anti-viral immune response, lymphocyte development and proliferation, MHC II antigen presentation, and adipocyte differentiation. Following conjugation to ATG12, the conjugate participates in the formation of autophagosome. ATG5 contributes to autophagic cell death by interacting with Fas-associated protein with death domain (FADD). The ATG5-ATG12 conjugate forms a cup-shaped isolation membrane that then detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may play a role in the apoptotic process, possibly within the modified cytoskeleton. Further, APG5 expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. The APG5-APG12 conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. Diseases associated with ATG5 dysfunction include spinocerebellar ataxia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

LncRNA LZTS1-AS1 induces proliferation, metastasis and inhibits autophagy of pancreatic cancer cells through the miR-532 /TWIST1 signaling pathway.

Cancer cell international

Wu H,Li A,Zheng Q,Gu J,Zhou W

Tue Jul 04 00:00:00 EDT 2023

Rift Valley Fever Virus Nucleoprotein Triggers Autophagy to Dampen Antiviral Innate Immune Responses.

Journal of virology

Zhu X,Guan Z,Fang Y,Zhang Y,Guan Z,Li S,Peng K

Thu Apr 27 00:00:00 EDT 2023

Curcumin protects against doxorubicin induced oxidative stress by regulating the Keap1-Nrf2-ARE and autophagy signaling pathways.

Psychopharmacology

Liao D,Shangguan D,Wu Y,Chen Y,Liu N,Tang J,Yao D,Shi Y

Mon May 01 00:00:00 EDT 2023

Downregulation of FAPP2 gene induces cell autophagy and inhibits PI3K/AKT/mTOR pathway in T-cell acute lymphoblastic leukemia.

Hematological oncology

Yuan T,Wang J,Shi C,Wang Y,Xia B,Xu W,Yang H,Yang Y,Ye MT,Khalid S,Liang Y,Tian C,You MJ,Wang Y

Fri Apr 01 00:00:00 EDT 2022

Nascent Glycoproteome Reveals That N-Linked Glycosylation Inhibitor-1 Suppresses Expression of Glycosylated Lysosome-Associated Membrane Protein-2.

Frontiers in molecular biosciences

Cao X,Meng P,Shao Y,Yan G,Yao J,Zhou X,Liu C,Zhang L,Shu H,Lu H

Sat Jul 16 00:00:00 EDT 2022

ATG16L2 inhibits NLRP3 inflammasome activation through promoting ATG5-12-16L1 complex assembly and autophagy.

European journal of immunology

Wang D,Yuan T,Liu J,Wen Z,Shen Y,Tang J,Wang Z,Wu X

Mon Aug 01 00:00:00 EDT 2022

Vitamin D3 promotes autophagy in THP-1 cells infected with Mycobacterium tuberculosis.

Experimental and therapeutic medicine

Wu Y,Lin X,Song F,Xue D,Wang Y

Tue Mar 01 00:00:00 EST 2022

Reciprocal regulation of NRF2 by autophagy and ubiquitin-proteasome modulates vascular endothelial injury induced by copper oxide nanoparticles.

Journal of nanobiotechnology

Li N,Du H,Mao L,Xu G,Zhang M,Fan Y,Dong X,Zheng L,Wang B,Qin X,Jiang X,Chen C,Zou Z,Zhang J

Sat Jun 11 00:00:00 EDT 2022

Resveratrol alleviates enterotoxigenic Escherichia coli K88-induced damage by regulating SIRT-1 signaling in intestinal porcine epithelial cells.

Food & function

Luo X,Wu S,Jia H,Si X,Song Z,Zhai Z,Bai J,Li J,Yang Y,Wu Z

Mon Jul 04 00:00:00 EDT 2022

Icariside II suppresses the tumorigenesis and development of ovarian cancer by regulating miR-144-3p/IGF2R axis.

Drug development research

Yuan D,Guo T,Qian H,Ge H,Zhao Y,Huang A,Wang X,Cao X,Zhu D,He C,Yu H

Thu Sep 01 00:00:00 EDT 2022

Oncostatin M receptor regulates osteoblast differentiation via extracellular signal-regulated kinase/autophagy signaling.

Stem cell research & therapy

Zhou J,Yang J,Dong Y,Shi Y,Zhu E,Yuan H,Li X,Wang B

Tue Jun 28 00:00:00 EDT 2022

RhFGF21 Protects Epidermal Cells against UVB-Induced Apoptosis through Activating AMPK-Mediated Autophagy.

International journal of molecular sciences

Zhao Y,Lin J,Li J,Bwalya C,Xu Y,Niu Y,Zhang Y,Wu J,Xu Y,Chen J,Ye S,Lin L

Tue Oct 18 00:00:00 EDT 2022

ZC3H4 regulates infiltrating monocytes, attenuating pulmonary fibrosis through IL-10.

Respiratory research

Liu Y,Zhang X,Wang J,Yang F,Luo W,Huang J,Chen M,Wang S,Li C,Zhang W,Chao J

Fri Aug 12 00:00:00 EDT 2022

Icaritin alleviates docetaxel-induced skin injury by suppressing reactive oxygen species via estrogen receptors.

Thoracic cancer

Zou J,Xu MX,Li F,Wang YH,Li XQ,Yu DJ,Ma YJ,Zhang YY,Sun XD

Sat Jan 01 00:00:00 EST 2022

Celecoxib activates autophagy by inhibiting the mTOR signaling pathway and prevents apoptosis in nucleus pulposus cells.

BMC pharmacology & toxicology

Chen W,Yasen M,Wang H,Zhuang C,Wang Z,Lu S,Jiang L,Lin H

Thu Dec 01 00:00:00 EST 2022

Upregulation of FOXO1 contributes to lipopolysaccharide-induced pulmonary endothelial injury by induction of autophagy.

Annals of translational medicine

Zhao Y,Zhang H,Zhang SL,Wei J,Zheng LL,Du JK,Zhu XY,Jiang L,Liu YJ

Wed Jun 01 00:00:00 EDT 2022

Selective STAT3 Inhibitor Alantolactone Ameliorates Osteoarthritis via Regulating Chondrocyte Autophagy and Cartilage Homeostasis.

Frontiers in pharmacology

Pei W,Huang X,Ni B,Zhang R,Niu G,You H

Sat Oct 16 00:00:00 EDT 2021

Tetrachlorobenzoquinone exposure triggers ferroptosis contributing to its neurotoxicity.

Chemosphere

Liu Z,Lv X,Yang B,Qin Q,Song E,Song Y

Mon Feb 01 00:00:00 EST 2021

Cordycepin inhibits cell senescence by ameliorating lysosomal dysfunction and inducing autophagy through the AMPK and mTOR-p70S6K pathway.

FEBS open bio

Zuo SQ,Li C,Liu YL,Tan YH,Wan X,Xu T,Li Q,Wang L,Wu YL,Deng FM,Tang B

Fri Oct 01 00:00:00 EDT 2021

Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1.

Signal transduction and targeted therapy

Xiao W,Yeerken D,Li J,Li Z,Jiang L,Li D,Fu M,Ma L,Song Y,Zhang W,Zhan Q

Fri Apr 16 00:00:00 EDT 2021

Latcripin-7A from Lentinula edodes C_91-3 induces apoptosis, autophagy, and cell cycle arrest at G1 phase in human gastric cancer cells via inhibiting PI3K/Akt/mTOR signaling.

European journal of pharmacology

Din SRU,Nisar MA,Ramzan MN,Saleem MZ,Ghayas H,Ahmad B,Batool S,Kifayat K,Guo X,Huang M,Zhong M

Wed Sep 15 00:00:00 EDT 2021

A nonautophagic role of ATG5 in regulating cell growth by targeting c-Myc for proteasome-mediated degradation.

iScience

Li S,Zhang L,Zhang G,Shangguan G,Hou X,Duan W,Xi Y,Xu N,Zhang B,Dong J,Wang Y,Cui W,Chen S

Fri Nov 19 00:00:00 EST 2021

Fostered Nrf2 expression antagonizes iron overload and glutathione depletion to promote resistance of neuron-like cells to ferroptosis.

Toxicology and applied pharmacology

Liu Z,Lv X,Song E,Song Y

Sun Nov 15 00:00:00 EST 2020

Resveratrol promotes osteoblastic differentiation in a rat model of postmenopausal osteoporosis by regulating autophagy.

Nutrition & metabolism

Wang W,Zhang LM,Guo C,Han JF

Thu Apr 14 00:00:00 EDT 2022

Bioinformatics

Protein Aliases: APG 5; APG5 autophagy 5 like (APG5L); APG5-like; Apoptosis-specific protein; Apoptosis-specific protein (ASP); ATG5 autophagy related 5 homolog; Autophagy protein 5; autophagy-related 5

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Gene Aliases: 2010107M05Rik; 3110067M24Rik; APG5; APG5-LIKE; APG5L; ASP; ATG5; Atg5l; AW319544; C88337; hAPG5; Paddy

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UniProt ID: (Human) Q9H1Y0, (Mouse) Q99J83

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Entrez Gene ID: (Human) 9474, (Mouse) 11793, (Rat) 365601

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Function(s)

protein binding Atg8 ligase activity membrane trafficking regulatory protein

Process(es)

autophagosome assembly mitophagy blood vessel remodeling regulation of cytokine secretion involved in immune response C-terminal protein lipidation autophagy apoptotic process cellular response to nitrogen starvation response to fungus macroautophagy cellular homeostasis antigen processing and presentation of endogenous antigen negative regulation of protein ubiquitination aggrephagy negative stranded viral RNA replication vasodilation response to drug negative regulation of apoptotic process post-translational protein modification nucleophagy negative thymic T cell selection innate immune response otolith development regulation of release of sequestered calcium ion into cytosol ventricular cardiac muscle cell development heart contraction negative regulation of cell death protein lipidation involved in autophagosome assembly positive regulation of mucus secretion regulation of cilium assembly positive regulation of mitophagy negative regulation of reactive oxygen species metabolic process negative regulation of histone H4-K16 acetylation immune system process regulation of reactive oxygen species metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ATG5 Monoclonal Antibody (2D3B8)

ATG5 Antibody (66744-1-IG) in IHC (P)

Product Details

66744-1-IG

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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