Invitrogen

ATG7 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

5 Published Figures

6 References

View all (29) ATG7 antibodies

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Datasheet

Protocols

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FIGURE: 1 / 11

ATG7 Antibody (PA5-17216) in WB

Western blot was performed using Anti-ATG7 Polyclonal Antibody (Product # PA5-17216) and a 70 kDa band corresponding to ATG7 was observed across cell lines tested and increased upon Chloroquine treatment. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), HeLa treated with Chloroquine (50nM for 15 hr) (Lane 2), HCT 116 (Lane 3) and HCT 116 treated with Chloroquine (50nM for 15 hr) (Lane 4) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membr... View More

ATG7 Antibody in Immunoprecipitation (IP)

Product Image Gallery

ATG7 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using ATG7 Polyclonal Antibody (Product # PA5-17216), shows increased expression of proteins in HeLa and HCT 116 cell lines upon Chloroquine treatment. {TM}

Product Details

PA5-17216

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 6 publications 6 publications

Immunoprecipitation (IP)

3 µL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to the amino terminus of Atg7

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

8 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 100µg/mL BSA, 0.15M NaCl, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10984892

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The citrus flavonoid "Nobiletin" impedes STZ-induced Alzheimer's disease in a mouse model through regulating autophagy mastered by SIRT1/FoxO3a mechanism.

Inflammopharmacology

El-Maraghy SA,Reda A,Essam RM,Kortam MA

Published figure using ATG7 polyclonal antibody (Product # PA5-17216) in Western Blot

Sun Oct 01 00:00:00 EDT 2023

Knockdown of VDAC1 alleviates the cognitive dysfunction secondary to sepsis-associated encephalopathy.

American journal of translational research

Cai M,Du B,Si Y,Miao J,Ge J,Zhang J,Song J,Bao H

PA5-17216 was used in Western Blot to elucidate the underlying mechanisms where knockdown of voltage dependent anion channel (VDAC1) alleviates the cognitive dysfunction caused by sepsis-associated encephalopathy.

Thu Nov 18 00:00:00 EST 2021

Vasorin/ATIA Promotes Cigarette Smoke-Induced Transformation of Human Bronchial Epithelial Cells by Suppressing Autophagy-Mediated Apoptosis.

Translational oncology

Chen W,Wang Q,Xu X,Saxton B,Tessema M,Leng S,Choksi S,Belinsky SA,Liu ZG,Lin Y

Published figure using ATG7 polyclonal antibody (Product # PA5-17216) in Western Blot

Wed Jan 01 00:00:00 EST 2020

Synergistic anticancer effect of cisplatin and Chal-24 combination through IAP and c-FLIPL degradation, Ripoptosome formation and autophagy-mediated apoptosis.

Oncotarget

Shi S,Wang Q,Xu J,Jang JH,Padilla MT,Nyunoya T,Xing C,Zhang L,Lin Y

PA5-17216 was used in western blot to investigate if Chal-24 can be combined with cisplatin for better cancer therapy

Fri Jan 30 00:00:00 EST 2015

A JNK-mediated autophagy pathway that triggers c-IAP degradation and necroptosis for anticancer chemotherapy.

Oncogene

He W,Wang Q,Srinivasan B,Xu J,Padilla MT,Li Z,Wang X,Liu Y,Gou X,Shen HM,Xing C,Lin Y

PA5-17216 was used in western blot to identify a novel anticancer mechanism that functions through autophagy-mediated necroptosis

Thu Jun 05 00:00:00 EDT 2014

Attenuation of TNFSF10/TRAIL-induced apoptosis by an autophagic survival pathway involving TRAF2- and RIPK1/RIP1-mediated MAPK8/JNK activation.

Autophagy

He W,Wang Q,Xu J,Xu X,Padilla MT,Ren G,Gou X,Lin Y

Published figure using ATG7 polyclonal antibody (Product # PA5-17216) in Western Blot

Sat Dec 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: APG7-like; ATG12-activating enzyme E1 ATG7; ATG7 autophagy related 7 homolog; autophagy related 7 homolog; autophagy-related 7; Autophagy-related protein 7; DKFZp434N0735; hAGP7; Ubiquitin-activating enzyme E1-like protein; Ubiquitin-like modifier-activating enzyme ATG7

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Gene Aliases: 1810013K23Rik; Agp7; APG7-LIKE; APG7L; ATG7; Atg7l; Gm21553; GSA7

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UniProt ID: (Human) O95352, (Rat) Q641Y5, (Mouse) Q9D906

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Entrez Gene ID: (Human) 10533, (Rat) 312647, (Mouse) 74244

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Function(s)

ubiquitin activating enzyme activity protein binding transcription factor binding Atg12 activating enzyme activity Atg8 activating enzyme activity protein homodimerization activity molecular_function small protein activating enzyme activity ubiquitin-protein ligase

Process(es)

cellular protein modification process protein lipidation C-terminal protein lipidation cellular response to nitrogen starvation cellular response to starvation positive regulation of autophagy protein transport macroautophagy positive regulation of macroautophagy protein ubiquitination positive regulation of protein modification process piecemeal microautophagy of nucleus suppression by virus of host autophagy positive regulation of apoptotic process late nucleophagy positive regulation of protein catabolic process defense response to virus membrane fusion cellular response to hyperoxia regulation of mitophagy mitophagy liver development cellular amino acid metabolic process autophagy organelle organization mitochondrion organization adult walking behavior post-embryonic development cerebellar Purkinje cell layer development pyramidal neuron development central nervous system neuron axonogenesis cerebral cortex development protein catabolic process neuron projection development regulation of protein ubiquitination protein modification by small protein conjugation negative stranded viral RNA replication response to starvation negative regulation of apoptotic process neurological system process cardiac muscle cell development membrane organization positive regulation of mucus secretion negative regulation of histone H4-K16 acetylation autophagosome assembly transport regulation of cell development negative regulation of cell death regulation of hemopoiesis

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ATG7 Polyclonal Antibody

ATG7 Antibody (PA5-17216) in WB

Product Details

PA5-17216

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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