Invitrogen

ATG7 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

View all (29) ATG7 antibodies

Questions & Answers

Questions & Answers

ATG7 Antibody in Immunocytochemistry (ICC/IF)

ATG7 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

ATG7 Antibody (PA5-35203) in ICC/IF

Immunocytochemistry analysis of ATG7 in U251 cells. Samples were incubated with ATG7 polyclonal antibody (Product # PA5-35203) using a dilution of 1:200 for 2 hr at room temperature followed by Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) at a dilution of 1:1,000 for 1hr. Cells were treated with Chloroquine (50 μM, 16hr), then fixed with 4% PFA (20 min), and permeabilized with Triton X-100 (0.2%, 30 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/mL, 5 min). ATG7 immunoreactivity is localized to autopha... View More

ATG7 Antibody in Immunocytochemistry (ICC/IF)

ATG7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ATG7 Antibody in Western Blot (WB)

ATG7 Antibody in Western Blot (WB)

ATG7 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

ATG7 Antibody in Western Blot, Immunohistochemistry, Immunohistochemistry (PFA fixed) (WB, IHC, IHC (PFA))

ATG7 Antibody in Western Blot (WB)

Product Image Gallery

ATG7 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of ATG7 in U251 cells. Samples were incubated with ATG7 polyclonal antibody (Product # PA5-35203) using a dilution of 1:200 for 2 hr at room temperature followed by Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) at a dilution of 1:1,000 for 1hr. Cells were treated with Chloroquine (50 μM, 16hr), then fixed with 4% PFA (20 min), and permeabilized with Triton X-100 (0.2%, 30 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/mL, 5 min). ATG7 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

ATG7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ATG7 in paraformaldehyde-fixed, paraffin-embedded human liver tissue sections. Samples were incubated with ATG7 polyclonal antibody (Product # PA5-35203) using a dilution of 1:25 for 1 hours at 37°C followed by an undiluted biotinylated goat polyvalent antibody. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6).

ATG7 Antibody in Western Blot (WB) — Western blot analysis of ATG7 in various lysates. Samples were incubated with ATG7 polyclonal antibody (Product # PA5-35203) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: Ba/F3 whole cell lysate; Lane 2: Caki-1 whole cell lysate; Lane 3: HepG2 whole cell lysate. Predicted band size: 78 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

ATG7 Antibody in Western Blot (WB) — Western blot analysis of ATG7 in 293 cell line lysates. Samples were incubated with ATG7 polyclonal antibody (Product # PA5-35203). Lysates at 2 µg/lane. hAPG7L-R509 (arrow).

ATG7 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Fig. 2 Abnormal autophagy in Atg7-deficient OHCs. A Representative immunostaining images showing efficient knockout of Atg7 in the OHCs and not in the IHCs. B Western blot showed that Atg7 was largely suppressed in the cochlear whole mount of Prestin Cre/+ Atg7 flox/flox mice. C Representative immunoblots showing elevated P62, increased LC3-I, and decreased LC3-II/LC3-I ratio. D P62 accumulated in Atg7-deficient OHCs, showing a colorful mosaic pattern when co-stained with myosin 7a. E P62 aggregated in the mutant OHCs, especially at middle and basal turns. An OHC in the basal turn was extruded from the sensory epithelium (white arrowhead). N = 6 for each group (western blot: N = 3). Scale bar: 10 mum.

ATG7 Antibody in Western Blot, Immunohistochemistry, Immunohistochemistry (PFA fixed) (WB, IHC, IHC (PFA)) — Fig. 1 OHCs and IHCs expressed the highest levels of Atg7 in the cochlea. A Representative immunoblot of Atg7 protein in the cochlea. B Expression of the Atg7 gene was analyzed by RT-PCR. C Atg7 was evident in OHCs and IHCs in the cochlear cryosections. D , E Atg7 puncta were distributed throughout the OHC cell bodies as indicated in the infracuticular, nuclear, and basal pool sections. N = 6 for each group (western blot: N = 3). Scale bar: A - C : 5 mum; D , E : 10 mum.

ATG7 Antibody in Western Blot (WB) — FIGURE 3 Representative pictures of effect of I/R on DNA damage, cell survival, and inflammation. (A) DNA gel electrophoresis diagram. (B) The phosphorylation of H2AX in response to I/R-induced DNA damage ( *** P < 0.001 vs. control). Western blot analysis expression level of LC3 I/II ( * P < 0.05) (C) , Beclin1 ( *** P < 0.001), SQSTM1 ( * P < 0.05), and ATG7 ( ** P < 0.01) (D) and lysosomal associated proteins Lamp1 ( ** P < 0.01), Lamp2 ( * P < 0.05), and Cathepsin B ( * P < 0.05) (E) in total protein extract from RVA of I/R treated vs. control group. (F) The expression of NLRP3 ( *** P < 0.001), Caspase-1 ( ** P < 0.01), ASC ( ** P < 0.01), and IL-1beta ( ** P < 0.01). Results represent mean +- SE ( n = 3). The graph shows the densitometry quantification of western blot bands. Here control group represented Sham group. * P < 0.05, ** P < 0.01, and *** P < 0.001.

ATG7 Antibody — FIGURE 3 Representative pictures of effect of I/R on DNA damage, cell survival, and inflammation. (A) DNA gel electrophoresis diagram. (B) The phosphorylation of H2AX in response to I/R-induced DNA damage ( *** P < 0.001 vs. control). Western blot analysis expression level of LC3 I/II ( * P < 0.05) (C) , Beclin1 ( *** P < 0.001), SQSTM1 ( * P < 0.05), and ATG7 ( ** P < 0.01) (D) and lysosomal associated proteins Lamp1 ( ** P < 0.01), Lamp2 ( * P < 0.05), and Cathepsin B ( * P < 0.05) (E) in total protein extract from RVA of I/R treated vs. control group. (F) The expression of NLRP3 ( *** P < 0.001), Caspase-1 ( ** P < 0.01), ASC ( ** P < 0.01), and IL-1beta ( ** P < 0.01). Results represent mean +- SE ( n = 3). The graph shows the densitometry quantification of western blot bands. Here control group represented Sham group. * P < 0.05, ** P < 0.01, and *** P < 0.001. {TM}

Product Details

PA5-35203

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:25

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 494-523 amino acids from human ATG7

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography, Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2552513

Product Specific Information

This antibody is predicted to react with rat based on sequence homology.

Target Information

This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Disruption of Atg7-dependent autophagy causes electromotility disturbances, outer hair cell loss, and deafness in mice.

Cell death & disease

Zhou H,Qian X,Xu N,Zhang S,Zhu G,Zhang Y,Liu D,Cheng C,Zhu X,Liu Y,Lu L,Tang J,Chai R,Gao X

PA5-35203 was used in Western Blot, Immunohistochemistry to show that OHCs orchestrate intricate nonselective and selective autophagic/mitophagy pathways working in concert to maintain cellular homeostasis.

Sat Oct 24 00:00:00 EDT 2020

Disruption of Atg7-dependent autophagy causes electromotility disturbances, outer hair cell loss, and deafness in mice.

Cell death & disease

Zhou H,Qian X,Xu N,Zhang S,Zhu G,Zhang Y,Liu D,Cheng C,Zhu X,Liu Y,Lu L,Tang J,Chai R,Gao X

PA5-35203 was used in Western Blot, Immunohistochemistry to show that OHCs orchestrate intricate nonselective and selective autophagic/mitophagy pathways working in concert to maintain cellular homeostasis.

Sat Oct 24 00:00:00 EDT 2020

Disruption of Atg7-dependent autophagy causes electromotility disturbances, outer hair cell loss, and deafness in mice.

Cell death & disease

Zhou H,Qian X,Xu N,Zhang S,Zhu G,Zhang Y,Liu D,Cheng C,Zhu X,Liu Y,Lu L,Tang J,Chai R,Gao X

PA5-35203 was used in Western Blot, Immunohistochemistry to show that OHCs orchestrate intricate nonselective and selective autophagic/mitophagy pathways working in concert to maintain cellular homeostasis.

Sat Oct 24 00:00:00 EDT 2020

Disruption of Atg7-dependent autophagy causes electromotility disturbances, outer hair cell loss, and deafness in mice.

Cell death & disease

Zhou H,Qian X,Xu N,Zhang S,Zhu G,Zhang Y,Liu D,Cheng C,Zhu X,Liu Y,Lu L,Tang J,Chai R,Gao X

PA5-35203 was used in Western Blot, Immunohistochemistry to show that OHCs orchestrate intricate nonselective and selective autophagic/mitophagy pathways working in concert to maintain cellular homeostasis.

Sat Oct 24 00:00:00 EDT 2020

Disruption of Atg7-dependent autophagy causes electromotility disturbances, outer hair cell loss, and deafness in mice.

Cell death & disease

Zhou H,Qian X,Xu N,Zhang S,Zhu G,Zhang Y,Liu D,Cheng C,Zhu X,Liu Y,Lu L,Tang J,Chai R,Gao X

PA5-35203 was used in Western Blot, Immunohistochemistry to show that OHCs orchestrate intricate nonselective and selective autophagic/mitophagy pathways working in concert to maintain cellular homeostasis.

Sat Oct 24 00:00:00 EDT 2020

Metformin alleviates hyperglycemia-induced endothelial impairment by downregulating autophagy via the Hedgehog pathway.

Autophagy

Niu C,Chen Z,Kim KT,Sun J,Xue M,Chen G,Li S,Shen Y,Zhu Z,Wang X,Liang J,Jiang C,Cong W,Jin L,Li X

PA5-35203 was used in Immunohistochemistry-immunofluorescence to describe that metformin downregulates HGtriggered autophagy through activating the Hh signaling pathway in a GLI1-dependent manner.

Wed May 01 00:00:00 EDT 2019

Chick Embryo: A Preclinical Model for Understanding Ischemia-Reperfusion Mechanism.

Frontiers in pharmacology

Fauzia E,Barbhuyan TK,Shrivastava AK,Kumar M,Garg P,Khan MA,Robertson AAB,Raza SS

Published figure using ATG7 polyclonal antibody (Product # PA5-35203) in Western Blot

Thu Oct 01 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: APG7-like; ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7; DKFZp434N0735; hAGP7; Ubiquitin-activating enzyme E1-like protein; Ubiquitin-like modifier-activating enzyme ATG7

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Gene Aliases: 1810013K23Rik; Agp7; APG7-LIKE; APG7L; ATG7; Atg7l; Gm21553; GSA7

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UniProt ID: (Human) O95352, (Mouse) Q9D906

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Entrez Gene ID: (Human) 10533, (Mouse) 74244

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ubiquitin activating enzyme activity protein binding transcription factor binding Atg12 activating enzyme activity Atg8 activating enzyme activity protein homodimerization activity small protein activating enzyme activity ubiquitin-protein ligase

Process(es)

cellular protein modification process protein lipidation C-terminal protein lipidation cellular response to nitrogen starvation cellular response to starvation positive regulation of autophagy protein transport macroautophagy positive regulation of macroautophagy protein ubiquitination positive regulation of protein modification process piecemeal microautophagy of nucleus suppression by virus of host autophagy positive regulation of apoptotic process late nucleophagy positive regulation of protein catabolic process defense response to virus membrane fusion cellular response to hyperoxia regulation of mitophagy autophagosome assembly mitophagy liver development cellular amino acid metabolic process transport autophagy organelle organization mitochondrion organization adult walking behavior post-embryonic development cerebellar Purkinje cell layer development pyramidal neuron development central nervous system neuron axonogenesis cerebral cortex development protein catabolic process neuron projection development regulation of protein ubiquitination protein modification by small protein conjugation negative stranded viral RNA replication response to starvation negative regulation of apoptotic process neurological system process cardiac muscle cell development regulation of cell development negative regulation of cell death membrane organization positive regulation of mucus secretion regulation of hemopoiesis negative regulation of histone H4-K16 acetylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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