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ATG7 Antibody (PA5-85388) in WB
Western Blot analysis of ATG7 was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ATG7 Polyclonal Antibody (Product # PA5-85388) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Product Details
PA5-85388
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ATG7.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2792530
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (92%), Rat (92%), Pig (96%), Chicken (82%), Rhesus Monkey (98%), Bovine (94%).
Positive Control: Target recombinant protein.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APG7-like; ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7; DKFZp434N0735; hAGP7; Ubiquitin-activating enzyme E1-like protein; Ubiquitin-like modifier-activating enzyme ATG7
Gene Aliases: 1810013K23Rik; Agp7; APG7-LIKE; APG7L; ATG7; Atg7l; Gm21553; GSA7
UniProt ID: (Human) O95352, (Mouse) Q9D906
Entrez Gene ID: (Human) 10533, (Mouse) 74244
cellular protein modification process
protein lipidation
C-terminal protein lipidation
cellular response to nitrogen starvation
cellular response to starvation
positive regulation of autophagy
protein transport
macroautophagy
positive regulation of macroautophagy
protein ubiquitination
positive regulation of protein modification process
piecemeal microautophagy of nucleus
suppression by virus of host autophagy
positive regulation of apoptotic process
late nucleophagy
positive regulation of protein catabolic process
defense response to virus
membrane fusion
cellular response to hyperoxia
regulation of mitophagy
autophagosome assembly
mitophagy
liver development
cellular amino acid metabolic process
transport
autophagy
organelle organization
mitochondrion organization
adult walking behavior
post-embryonic development
cerebellar Purkinje cell layer development
pyramidal neuron development
central nervous system neuron axonogenesis
cerebral cortex development
protein catabolic process
neuron projection development
regulation of protein ubiquitination
protein modification by small protein conjugation
negative stranded viral RNA replication
response to starvation
negative regulation of apoptotic process
neurological system process
cardiac muscle cell development
regulation of cell development
negative regulation of cell death
membrane organization
positive regulation of mucus secretion
regulation of hemopoiesis
negative regulation of histone H4-K16 acetylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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